Antioxidants ; metabolism ; therapeutic use ; Bronchopulmonary Dysplasia ; drug therapy ; etiology ; metabolism ; Humans ; Hyperoxia ; complications ; Infant, Newborn ; Lung ; metabolism ; Reactive Oxygen Species ; metabolism

ObjectiveTo obtain the soluble expression of P protein of norovirus(NV)G Ⅱ. 2[P16]and analyze its antigenic characteristics.MethodsThe recombinant plasmid GⅡ.2-NV-pET-28a was constructed by optimizing the NV GⅡ.2[P16]P protein gene,synthesizing and cloning it into pET-28a(+)vector. The recombinant plasmid was transformed into DH5α competent cells,and the expression strain was optimized to make the P protein express in soluble form. The expressed P protein was purified by Ni-NTA affinity chromatography,of which the antigenicity was detected by Western blot,the purity was detected by size exclusion high performance liquid chromatography(SEC-HPLC),and the antigenantibody binding ability was identified by ELISA.ResultsThe target protein of recombinant BL21 Star(DE3)pLySs was highly expressed in soluble form. After purification by affinity chromatography,the purity of GⅡ.2 P protein was 95. 53%,and the antigenicity was good.ConclusionSoluble NV P protein was successfully prepared by prokaryotic expression strain,and the antigenicity of P protein was confirmed,which laid a foundation of the development of NV G Ⅱ.2[P16]detection kits and the recombinant subunit vaccines.

Objective To study the pathogenic molecular mechanism of H.pylori through analyzing its effect of on MMP-9 expression. Methods BGC-823 cells were cocultured with ATCC49503(CagA +)and HP 030811 (CagA -) H.pylori strains respectively, then the total cellelar RNA was extracted. The MMP-9 mRNA expression in BGC-823 cells, 9 cases of H.pylori-positive and 9 cases of H.pylori-negative gastric ulcer biopsy tissues was detected by RT-PCR. The MMP-9 protein expression on the paraffin-imbedded tissue sections of 17 cases of H.pylori-positive and 14 cases of H.pylori-negative gastric ulcer tissues was detected with immunohistochemical technique, and was quantified by image quantitative analysis. Results H.pylori induced MMP-9 expression in BGC-823, and the level of MMP-9 expression induced by CagA -postive H.pylori strain was higher than that by CagA -negative one. MMP-9 expression level was higher in the H.pylori-positive gastric ulcer tissues than that in the H.pylori-negative ones. Conclusion H.pylori-induced MMP-9 expression may play an important role in gastric ulcer formation and gastric carcinogenesis.

Objective To explore the therapeutic mechanism of Xiao Chaihu Decoction for endometriosis in rats. Methods The rat models of endometriosis were treated with Xiao Chaihu Decoction, Danazol, and Xiao Chaihu Decoction plus Danazol for 4 weeks,respectively. And then cyclooxygenase-2(COX-2) expression in endometrium and endometriotic tissue was observed by immunohistochemistry method. Results The expression of COX-2 in the endometriotic tissue was lower than that in endometrium in Xiao Chaihu Decoction group and Xiao Chaihu Decoction plus Danazol group, while the results in Danazol group and control group were opposed to those in the above two groups. Conclusion The mechanism of Xiao Chaihu Decoction for rats endometriosis may be related to the decrease of COX-2 expression in ectopic Endometrium, thus reducing the formation of prostaglandins.

Objective To observe the clinical effect of modified Hezhong Powder for the treatment of children mesentetic lymphadenitis.Methods Sixty children with mesentetic lymphadenitis were randomly divided into treatment group and control group,30 cases in each group.The control group was treated routine anti-inflammatory drug Cefaclor for Oral Suspension,and was given symptomatic treatment when necessary.The treatment group was given modified Hezhong Powder on the basis of treatment for the control group.The treatment course lasted for two weeks.After treatment,clinical efficacy and safety,and effects on relieving primary and secondary symptoms and on shortening the swollen lymph nodes were observed.Results (1) The total effective rate of the treatment group was 96.7％,and that of the control group was 83.3％,the difference being significant (P ＜ 0.05).(2) The treatment group had better effect on relieving the primary symptom of abdominal pain,and secondary symptoms of poor appetite,vomiting,abdominal distention and abnormal stool than the control group.The differences of time for the symptoms disappearing were significant (P ＜ 0.05).(3) After treatment,the swollen.lymph nodes of the two groups were shrunk(P ＜ 0.05),and the shrinkage in the treatment group was superior to that of the control group (P ＜0.05).(4) During the treatment,no obvious adverse reaction occurred in the two groups.Conclusion Modified Hezhong Powder combined with western medicine is effective and safe for the treatment of children mesentetic lymphadenitis,and its efficacy is superior to that of the western medicine alone.

Objective To investigate the clinical efficacy and safety of laparoscopic appendectomy for acute appendicitis.Methods Nighty-two cases of acute appendicitis were included in this study,randomly divided into observation group (n =46) and control group (n =46) according to the random number table method.The observation group was treated with laparoscopic appendectomy,and control group with the traditional open appendectomy treatment.We compared the two groups of cases in postoperative conditions and the level of WBC changes and 1d and 3d after operation.Results The operation time of the observation group was shorter than that of the control group (t=4.049,P＜0.05);there was no significant difference in lymph nodes (t=0.570,P＞0.05);the amount of bleeding was less than that of the control group (t=20.748,P＜0.05).The get-out-of-bed activity time in the observation group was faster than the control group (t=9.221,P＜0.05);eating time was faster than the control group (t=8.794,P＜0.05);the anus recovery exhaust time was faster than the control group (t=17.423,P＜0.05);hospital stay was shorter than the control group (t=13.479,P＜0.05);the complication rate was lower than that of the control group (t=13.883,P＜0.05).The levels of 1d and 3d WBC were lower in the observation group than that in the preoperative group (t=7.277,26.911,P＜0.05);the levels of 1d and 3d WBC in the observation group were lower than those in the control group at the same time (t=3.6189,17.827,P＜0.05);the 3d WBC level of the observation group was lower than that of 1d (t=20.732,P＜0.05).Conclusion Laparoscopic appendectomy is a safe and effective treatment for patients with acute appendicitis,and it is worthy of further clinical research.

Five stagnations are an important content of Plain Questions.Many doctors think highly of the treating principle,while overlook the other content.Through researches on Plain Questions,the author lays out the entire system,including the prevention and treatment for mild stagnation,the presymptom,changes on phenology,climate and human body for severe stagnation.

Objective: To establish a determination for psoralen and isopsoralen in Longbishu Capsules (Fructus Psoraleae, Herba Leonuri, Succinum, Pseudobulbus Cremastrae seu pleiones, Herba Lysimachiae, Spora Lygodii). Methods : TLC-Scanning was used. Results : The average recovery of psoralen was 97.4% and RSD was 3.06%,respectively, and isopsoralen was 97.6% and RSD was 2.72%,respectively. Conclusion : The method can be used for quality control of Longbishu Capsules.

0.05). Conclusion The LDH method applied in the in vitro inhibition assay for P. falciparum seems simple, reliable and has generated a satisfactory result.