Objective: Astragalus mongholicus could promote angiogenesis.Microemulsion has been widely used in the researches of traditional Chinese medicine in recent years.This study aimed to investigate the effect of Astragalus mongholicus microemulsion modified-collagen on angiogenesis of rats in vivo.Methods: Fifty SD rats were randomly divided into 5 groups: blank collagen,blank microemulsion modified-collagen,vascular endothelial cell growth factor(VEGF) modified-collagen,Astragalus mongholicus microemulsion modified-collagen and Astragalus mongholicus microemulsion plus VEGF modified-collagen.Then four collagen samples were imbedded in each rat.After the establishment of the models,the rats were executed at 3,7 and 14 days.The weights of the collagen samples were measured,and their hematoglobin and hydroxyproline levels were determined by the hemiglobincyanide colorimetric and sampler alkaline hydrolysis methods.The color of the wound surface was observed and the surrounding tissues were examined by HE staining for those killed at 14 days.Results: Both Astragalus mongholicus microemulsion and Astragalus mongholicus microemulsion plus VEGF significantly increased the production of hematoglobin and hydroxyproline(P0.05).Conclusion: Astragalus mongholicus microemulsion might promote angiogenesis by increasing the number of micrangia and the production of hematoglobin and hydroxyproline.

Objective To explore the relationship between acute organophosphate poisoning and atrial fibrillation (AF). Methods Three hundred and twenty-two acute organophosphorus poisoning of elderly patients treated from January 2000 to June 2008 in our hospital were analyzed. Among these patients, 36 cases with AF were selected as case group and 38 age-gender-matched cases without AF were selected as control group. Serum cholinesterase (ChE), myocardial damage markers such as creatine kinase (CK), creatine kinase-MB isoenzyme (CK-MB) and Troponin- Ⅰ (cTn-Ⅰ) were compared between the two groups. Results ChE was decreased in the two groups, and ChE was lower in case group than in control group [(1126. 42±1047.02) vs. (1604.82±934.06)U/L, t= 2.07, P=0. 0414]. CK, CK-MB and cTn-Ⅰ were increased in different degree in the two groups. There was no difference in CK between the two groups [(609.97±597.84) U/L vs. (462.84± 530. 71)U/L, t= 1. 121, P= 0. 266]. CK-MB and cTn-I were higher in case group than in control group [(97.31±104.50) vs. (55. 16±69.62)U/L, t=2. 052,P=0. 0438; (2.39±0. 88) vs. (1.81± 1.38) μg/L, t = 2. 132, P = 0. 036, respectively9 . Conclusions AF caused by acute organophosphate poisoning may be related to the decrease of ChE activity, acetylcholine accumulation and myocardial injury.

ObjectiveTo study the feasibility,safety and effect of laparoscopic splenectomy and selective pericardial devascularization in the treatment of portal hypertension.MethodsThis study included 7 patients diagnosed as posthepatitie cirrhosis and portal hypertension with a history of up GI bleeding receiving laparoscopic splenectomy and selective pericardial devascularization.ResultsThe operation was successful in all 7 patients without conversion to laparotomy.There were no postoperative severe complications.The average operation time was 4.2 h,mean blood loss was 430 ml.All patients recovered well,and were followed-up for 0.5 ～ 2.0 years,and there was no rebleeding in this period.ConclusionsLaparoscopic selective pericardial devascularization is feasible,safe,minimally invasive.The short to mid term result was satisfactory.

Acute Kidney Injury ; chemically induced ; Aristolochia ; adverse effects ; Drug Therapy, Combination ; Drugs, Chinese Herbal ; adverse effects ; therapeutic use ; Humans ; Male ; Middle Aged ; Nephrotic Syndrome ; drug therapy ; Phytotherapy

Objective To evaluate the effect of a community-based nutrition education program on the dietary quality of the elderly with diet balance index(DBI). Methods Eighty-three volunteers from Zhujing Town of Jinshan District of Shanghai with age ≥60 years were served as study objectives,and a total of 70 people completed the program.They were given nutritional guidance bimonthly in combination with measures such as community publications,panel discussions and individual interviews.Measurements of dietary intakes,which lasted for 3 d, were performed before intervention and 12 months after intervention,respectively.Diet quality was evaluated with DBI scoring system,and the parameters were compared before and after intervention. Results There were significant differences in DBI-Total Score(DBI-TS),DBI-Lower Bound Score(DBI-LBS)and DBI-Diet Quality Distance(DBI-DQD)before and after intervention for these 70 people (P<0.05).The proportion of subjects with adequate milk and soy product consumption increased from 7.1% before intervention to 20.0% after intervention,that with proper amount of salt intake from 22.9%to 51.5%.and that with diverse diet from 20.0%to 49.9%.The major DBI pattern changed from pattern B (47.1%) and pattern E(34.3%)to pattern B (34.3%) and pattern A (32.9%). Conclusion The community-based nutrition education program for the elderly can improve the diet quality of the elderly.

Acupuncture Points ; Acupuncture Therapy ; Humans ; Male ; Meige Syndrome ; therapy ; Middle Aged

Objective To explore methods of diagnostic and treatment for renal cortical abscess to reduce its misdiagnosis.Methods Eleven cases of renal cortical abscess diagnosed and treated at Beijing International Airport Hospital during 1991 to 2003 were reviewed and analyzed.Results Among the 11 patients with renal cortical abscess,diagnosis was established in nine(82%)before treatment,seven were cured by incision and drainage,one was cured by incision and drainage only after failure in percutaneous nephropuncture,and one was cured by intravenous injection of antibiotics.Two cases(18%)were misdiagnosed as renal cancer and radical nephrectomy were performed for them.Conclusions Diagnosis for renal cortical abscess should be based on combination of patients clinical manifestation,laboratory examinations and imaging findings.Incision and drainage is the choice of treatment for renal cortical abscess.

Objective To observe the clinical effect of herbal decoction Sishen Jian on acute osteoarthritis of knee joint.Methods The 60 osteoarthritis cases in the attack stage were randomized into treatment group(30 cases)and control group(30 cases).The treatment group was given the decoction Sishen Jian and Diclofenac Sodium Sustained Release Capsules and the control group was given the capsules only.Two weeks made one course of treatment.Before and after treatment,the symptoms such as pain and morning stiff- ness,the signs including joint swelling and tenderness,and the erythrocyte sedimentation rate(ESR)and C reactive protein were ob- served for comparison.Results The total effective rate of the treatment group and control group was 96.66% and 53.26% respec- tively.The difference of two groups was significant(P

Adolescent ; Adult ; Child ; Ear Auricle ; surgery ; Female ; Humans ; Male ; Postoperative Complications ; surgery ; Reconstructive Surgical Procedures ; Skin Transplantation ; Surgical Flaps ; Young Adult

Objective To investigate the characteristics, degradation and biocompatibility of a woven chitosan fiber (CF). Methods Scanning electron microscopic observation, deteronination of density, degree of swelling and tensile strength were performed to characterize the CF. In vitro degradation was observed by immersing CF in buffered aqueous solution of pH 6 containing lysozyme at 37 ℃, while in vivo degradation was studied after intramuscular implantation of CF in rats. Furthermore, rat mesenchymal stem cells were directly cultured on CF to estimate its toxicity. Results The diameter of CF was about 2000?63?m, with a sleek surface. The density and rate of swelling were 1.093?0.27g/cm3 and 2.30?0.21, respectively. The tensile strength was 80.18?1.56MPa. Specific viscosity, measured after the addition of lysozyme to the solution, decreased dramatically within 2 hours compared with that of the control mixture without enzyme. There was obvious inflammatory reaction after intramuscular implantation in the first week after surgery, bat inflammation subsided gradually. Biodegradation was not obvious within 4 weeks, but majority of it was absorbed in twelve weeks. Cells grew well on it with normal morphology, and no inhibition of cell proliferation could be observed. Conclusion Woven CF has a good tensile strength and fairly good biodegradation and biocompatibility, and it can be used in bone and cartilage tissue engineering.