OBJECTIVE To discuss the function of sustaining quality improvement in preventing perineum wound infection.METHODS The perineum wound infection situation in 2003 was retrospectively analyzed,and since then the measures for sustaining quality improvement were implemented.RESULTS In 2004 and in 2005 the perineum wound infection percentage and the stitch inflammation rate were obviously reduced than in 2003.The difference was statisticaly significant.CONCLUSIONS Sustaining quality improvement is an unceasing,gradually advancing and long-term process.The prevention of perineum wound infection is a long time work for obstetrics workers.To carry out strictly the aseptic technical operation,grasp skilled suture technology,choose easily absorbable suture,maintain suitable cleanliness and nursing and use the antibiotic could be effectively to prevent the perineum wound infection and the stitch inflammation occurrence.

Aim: To provide the foundation for reasonable utilization by analyzing the essential oils of Sabina pingii var. wilsonii.. Methods: The essential oils were extracted using steam distillation and separated with GC capillary column. The components were quantitatively determined with normalization method, and were preliminarily identi-fied by GC-MS. Results: 79 Components were identified, which took up 93. 78% of the essential oils. Conclusion: The main components of essential oils were a-terpineol( 16. 70%); α-fenchene( 14. 98%); 2,6,10-dodecatrien-1-ol, 3,7, 11-trimethyl-( 6.49%); 1, 4-cyclohexadiene, 1-methyl-4-[1-methylethyl]-( 4. 74%); kaura-5, 16-dien-18 [orl9]-ol(3. 62%); naphthalenemethanol, 1, 2, 3, 4, 4a, 5, 6, 7-octahydro-α, α, 4a, 8-tetramethyl-, [2R-cis]-(3. 04%); [ + ]-4-carene ( 2. 81%); D-limonene ( 2. 71%); β-myrcene ( 2. 59%); cedrol (2.40%); a-pinene (2. 36%); 7-isopropyl-1, 1, 4a-trimethyl-1 ,2,3,4,4a, 9,10,10a-octahydrophenanthrene(2. 32%).

Objective To explore the relationship between parenting stress and social support, cop-ing style in mothers of children with autism. Methods 105 mothers with autistic children(the observation group) and 100 mothers with healthy children (the control group) were investigated by questionnaires, which included parenting stress index-short form (PSI-SF), social support scale and simplified coping style questionnaire. And then the relationship between parenting stress, social support and coping style was ana-lyzed among them. Results Mothers of children with autism experienced a significantly higher level in the total score of parenting stress and all the domains when compared with mothers of healthy children. Par-enting stress was negatively correlated with social support. Conclusions Mothers of children with autism generally experience a significantly higher level than parents of healthy children in the total parenting stress and all the domains. Social support could reduce parenting stress of mothers with autistic children.

AIM:To study the expression of Jagged 2/Notch3 signaling molecules in pulmonary vascular wall of pulmonary hypertensive rats induced by monocrotaline .METHODS: SD rats were randomly divided into normal control group (C group,n=15), solvent control group (S group,n=15) and monocrotaline model groups (M group,n=15).The model of pulmonary hypertension was established by a single subcutaneous injection of monocrotaline (50 mg/kg).The rats in S group were given a single subcutaneous injection of the same dose of solvent .After 4 weeks, the pulmonary vascular remodeling was assessed by HE staining , and the mean pulmonary artery pressure ( mPAP) and right ventricular systolic pressure (RVSP) were determined by right heart catheterization .The expression of Jagged2/Notch3 /Hes5 molecules in the pulmonary vascular wall was detected by immunohistochemical method and real -time PCR.RESULTS:Compared with S group and C group , the percentage of medial wall thickness of smaller arteries in model group increased significantly (P<0.01).The levels of mPAP and RVSP in M group were significantly higher than those in S group and C groups (P<0.01).The results of real-time PCR showed that the expression of Jagged 2, Notch3 and Hes5 was significantly increased in M group compared with S group and C group .The data from immunohistochemical detection indicated that Jagged 2 mainly expressed in the intima of small lung artery , Notch3 and Hes5 mainly expressed in the medial smooth muscle cells .Com-pared with S group and C group , the expression of Jagged 2 and Notch3 was significantly increased in the lung small arteries of M group.CONCLUSION:The activation of Jagged2/Notch3 signaling pathway might play an important role in the for-mation of pulmonary hypertension .

Objective To discuss the value of detection of small liver tumor with Galactose-Bovine-serum-albumin containing superparamagetic(Gal-BSA-SPIO) in rabbits primarily.Methods Gal-BSA-SPIO was obtained by stabilizing small superparamagnetic iron with galactose-bovine-serum-albumin,and its size was measured.Models of liver tumor(diameter＜1.5 cm) in rabbits were founded.The animals were randomly divided into groups of SPIO (n=10) and group of Gal-BSA-SPIO (n=10).Each group was further subdivided as 5 μmol Fe/kg group (n=5) and 10 μmol Fe/kg group (n=5),respectively.All animals were performed magnetic resonance imaging before and after administration of agents according to groups.SE T2WI,FSE T2WI and GRE T2WI sequences were used.Then signal of noise(SNR) of liver and contrast-noise ratio(CNR) of tumor-liver were measured and compared statistically.The histopathology study was performed for liver and tumor specimens after MR imaging.Results The mean value for the volume size was 34.4 nm,and the core particle size was 14.8 nm.The differences of liver SNR between before and after enhancement for 5 μmol Fe/kg SPIO,5 μmol Fe/kg Gal-BSA-SPIO,10 μmol Fe/kg SPIO and 10 μmol Fe/kg Gal-BSA-SPIO group were (1.92±0.42),(3.75±0.51),(3.25±0.72) and (4.73±0.34) on SE sequence,respectively;(1.56±0.18),(3.56±0.64),(3.33±0.16) and (4.83±0.16) on FSE sequence,respectively;(5.55±0.91),(9.58±0.66),(9.20±0.29) and (10.63±0.32) on GRE sequence,respectively.There were significant differences between all groups for difierent doses and agents and within all different sequences (P＜0.01).The differences of tumor-liver CNR between before and after enhancement of these 4 groups were(2.22±0.68),(5.27±0.22),(4.25±0.51) and (6.97±0.63) on SE sequence,respectively;(1.73±0.41),(4.46±0.54),(3.73±0.49)and (5.39±0.39) on FSE sequence,respectively;(5.98±0.71),(11.79±0.57),(9.57±0.54)and(14.64±1.35)on GRE sequence,respectively.The differences between all groups of different doses and agents and within all sequences showed significant (P＜0.01). Histopathological study revealed that Gal-BSA-SPIO was mainly distributed in hepatocytes while SPIO mainly in Kupffer cells.Conclusion Gal-BSA-SPIO is a agent with good negative enhancement effect in liver.It may be helpful for detecting small tumor of liver.

Objective To study the effect of lithium chloride on the gap junction in the myocardium under chronic hypoxia.Methods Twenty-five C57BL/6J mice were randomly divided into normoxia group,hypoxia group,normoxic control group,hypoxia + saline group and hypoxia + lithium chloride group.Hypoxia group was treated with 10％ oxygen concentration for 4 weeks.Hypoxia + saline group and hypoxia + lithium chloride group were intraperitoneal injection of saline and lithium chloride.Electrophysiology and cardiac catheterization were used to assess arrhythmias,heart rate and ejection fraction.The expression of Cx43,phosphorylated glycogen synthase kinase 3β(p-GSK-3β) and glycogen synthase kinase 3β (GSK-3β) were detected by Western blot.Results Compared with the normoxia group,the hypoxia group had a faster heart rate [(448 ± 18) bpm vs.(401 ± 13) bpm,P＜0.05),and the ejection fraction was decreased [(56±5)％ vs.73±4)％,P＜0.05],arrhythmia score increased [(3.4±0.5)％ vs.(0.6±0.5)％,P＜0.05],Cx43 expression was decreased.Compared to hypoxia + normal saline group,the heart rate decreased[(412±11)bpm vs.(454±18)bpm,P＜0.05],ejection fraction increased[(69±3)％ vs.(55±4)％,P＜0.05],the score of arrhythmia decreased [(1.8±0.4) ％ vs.(3.0±0.7)％,P＜0.05] in hypoxia + lithium chloride group,the expression of Cx43 and the rate of p-GSK-3β to GSK-3β were increased.Conclusion During the chronic hypoxia,lithium chloride can sustain the gap junction through inhibition of GSK-3β signaling way,which can also reduce the rate of arrhythmia.

Radiation exposure is an ongoing and serious threat in military and public health concern, and there is an unmet need for effective preventative or mitigative treatment against radiation-induced injuries. The handful of FDA approved radiation protection agents cannot be widely used due to their side effects. Some natural non-toxic compounds such as bee products have been reported to prevent and treat radiation-induced injuries of oral mucosa, esophagus, skin, liver, intestine and hemopoietic system by reducing radiation-induced oxidative stress, apoptosis and DNA damage, indicating that they may be potential options of safe radioprotective agents. In this paper, the experimental and clinical studies on prevention and treatment of radiation injury by bee products were reviewed.

Objective:To investigate the effects of miRNA-628-3p (miR-628-3p) on the proliferation, apoptosis and invasion of non-small cell lung cancer H1299 cells and its targeting relationship with insulin-like growth factor 1 receptor (IGF-1R).Methods:The blank control group (untreated H1299 cells), miR-NC group (H1299 cells transfected with empty plasmid), miR-628-3p-M group (H1299 cells transfected with miR-628-3p mimic sequence plasmid) and miR-628-3p-I group (H1299 cells transfected with miR-628-3p inhibitory sequence plasmid) were established. The cells in each group were cultured for 72 h, and the cell proliferation ability was detected by methyl thiazol tetrazolium (MTT) method, the number of cell monoclonal formation was determined by crystal violet staining, the level of cell apoptosis was determined by flow cytometry, and the cell invasion ability was determined by Transwell method. The mRNA levels of miR-628-3p and IGF-1R in cells were determined by real-time fluorescence quantitative reverse transcription polymerase chain reaction (qRT-PCR), and the protein level of IGF-1R in cells was determined by Western blotting.Results:Compared with the blank control group and miR-NC group, the cell survival rate [(42±7)% vs. (78±6)%, (76±7)%], the number of monoclonal formation [235±35 vs. 614±89, 618±75], the number of invasive cells [(265±85) cells vs. (693±185) cells, (703±119) cells], relative expression of IGF-1R mRNA (2.17±0.14 vs. 3.38±0.15, 3.37±0.13) and relative expression of IGF-1R protein (0.34±0.13 vs. 0.89±0.19, 0.88±0.18) in the miR-628-3p-M group were lower (all P < 0.05), but the apoptosis rate [(9.30±3.51)% vs. (3.30±1.54)%, (3.10±1.94)%] and relative expression of miR-628-3p (6.93±0.17 vs. 3.29±0.15, 3.30±0.16) were higher (all P < 0.05); the cell survival rate [(90±6)%], the number of monoclonal formation (1 063±102), the number of invasive cells [(1 985±426) cells], relative expression of IGF-1R mRNA (4.30±0.18) and relative expression of IGF-1R protein (1.47±0.17) in the miR-628-3p-I group were higher (all P < 0.05), but the apoptosis rate [(0.90±0.20)%] and the relative expression of miR-628-3p (1.93±0.18) were lower (both P < 0.05). Compared with the miR-628-3p-M group, the miR-628-3p-I group had higher cell survival rate, the number of monoclonal formation, the number of invasive cells, and the relative expressions of IGF-1R mRNA and protein (all P < 0.05), but the apoptosis rate and relative expression of miR-628-3p were lower (both P < 0.05). Conclusions:After regulation of miR-628-3p level, the proliferation, migration and invasion of H1299 cells are affected. miR-628-3p may have a targeting relationship with IGF-1R.

To effectively screen treating pigmentation disorders drug, a new transgenic zebrafish drug screening model was constructed. Green fluorescent protein(GFP)were drived by tyrosinase-related protein 1a (tyrp1a)promoter specific expression in melanocyte. Effect of N-Phenylthiourea(PTU)and alpha-melanaocyte stimulating hormone(α-MSH)on the GFP expression and melanogenic of tyrp1a: eGFP zebrafish were photographed under the steromicroscope. Results showed that PTU could significantly inhibit the melanogenic and expression of GFP of zebrafish. As compared with control group, α-MSH treatment resulted in marked stimulation of body pigmentation and expression of GFP. In conclusion, a new transgenic zebrafish drug screening model was successfully established, which can be used for treating pigmentation disorders.

Objective    To compare the effects of transthoracic device closure and surgical closure on ventricular septal defect systemically. Methods    A systematic literature search was conducted using the PubMed, EMbase, The Cochrane Library, VIP, CNKI, CBM, Chinese Clinical Trial Register, ClinicalTrials. gov and Wanfang Database up to July 31, 2016. Quality was assessed and data of included articles were extracted. The meta-analysis was conducted using RevMan 5.0 and Stata 14.0 software. Results    Eleven studies were identified, including 5 RCTs and 6 cohort studies involving 2 504 patients. For success rate, there was no statistical difference between the transthoracic closure group and the surgical closure group in RCT (RR=0.99, 95%CI 0.96 to 1.03, P=0.70); the success rate in the transthoracic closure group was lower than that in the surgical closure group in the cohort study (OR=0.21, 95%CI 0.08 to 0.55, P=0.002). Both  results of RCTs and cohort studies showed that compared with surgical closure, transthoracic device closure reduced duration of the operation (RCT MD=–79.38, 95%CI –95.00 to –63.76, P<0.000 01; cohort study MD=–66.26, 95%CI –71.20 to –61.31, P<0.000 01) and hospital stay (RCT MD=–2.10, 95%CI –2.65 to –1.55, P<0.000 01; cohort study MD=–3.99, 95%CI –6.03 to –1.94, P=0.000 1), and the patients with blood transfusion (RCT RR= 0.04, 95%CI 0.01 to 0.11, P<0.000 01; cohort study OR=0.01, 95%CI 0.00 to 0.13, P=0.001). In the transthoracic closure group the risk of postoperative arrhythmia reduced (RCT RR=0.20, 95%CI 0.13 to 0.32, P<0.000 01; cohort study OR=0.46, 95%CI 0.31 to 0.67, P<0.000 1). In the transthoracic closure group a higher postoperative valvular regurgitation risk in RCT induced (RR=1.45, 95%CI 1.07 to 1.96, P=0.02) and the rate of postoperative valvular regurgitation in cohort study reduced (OR=0.43, 95%CI 0.20 to 0.92, P=0.03). However, there was no statistical difference in postoperative residual shunt (RCT RR=0.96, 95%CI 0.57 to 1.62, P=0.89; cohort study OR=0.52, 95%CI 0.12 to 2.25, P=0.38). Conclusion    Transthoracic device closure can shorten duration of the operation, hospital stay and reduce the patients with blood transfusion and post- and intraoperative arrhythmia risk. Therefore, transthoracic device closure may be a better approach for some ventricular septal defect patients.