BACKGROUND: Osteophyte caused by degenerative osteoarthropathy of the cervical vertebrae may compress the vertebral artery, resulting in vertebrobasilar insufficiency, especially when the patients' head postures change, making them adapt forced head position.OBJECTIVE: To investigate successively the relationship between the change of flow velocity of the posterior circulation and the symptom of vertebrobasilar insufficiency during various head postures with transcranial Doppler ultrasound.DESIGN: Randomized grouping controlled, auto-control observation.SETTING: Department of Neurology, Guangxi Minzu Hospital and Department of Neurology, First Hospital, Peking University.PARTICIPANTS: A total of 20 patients with vertebrobasilar insufficiency and 10 normal persons were enrolled from Department of Neurology,Guangxi Minzu Hospital between March 2003 and May 2004.METHODS: Regard blood flow velocity in natural position of anterior segment of posterior cerebral artery as baseline values. Blood flow velocity was compared at left rotation, right rotation, anterior inflexion and posterior inflexion. Symptoms of vertebrobasilar insufficiency were observed. The data were collected and compared in individuals, within group, and between groups.MAIN OUTCOME MEASURES: Blood flow velocity difference of various head postures in each group; Symptoms of vertebrobasilar insufficiency were observed.RESULTS:During monitoring, there were 9 cases complained of ischemic symptoms and 11 cases without symptoms. There was no significant difference in blood flow change of left rotation, right rotation and posterior inflexion in the normal control group with natural position. The reduction of flow velocity was significant in anterior inflexion (P ＜ 0.05 ), and none appeared clinical symptom. There was significant difference in reduction of blood flow at anterior inflexion and at natural position in patients with symptom (P ＜ 0.05), and there was significant difference in reduction of blood flow at posterior inflexion and natural position (P ＜ 0.001 ). There was significant difference in reduction of blood flow at left rotation and posterior inflexion in those without symptom during monitoring as compared with that at natural position (P ＜ 0.05 ).CONCLUSION: Continuously observing the blood flow in posterior cerebral artery at different head position can objectively assess the relationship between decrease of blood flow velocity of vertebral arteries induced by neck movements and clinical symptoms.

Objective To evaluate the clinical utility of interferon-gamma (IFN-γ) in serum and cerebrospinal fluid in the diagnosis of tuberculous meningitis .Methods IFN-γlevels in serum and cerebrospinal fluid were analyzed by ELISA method in 25 patients with tuberculous meningitis ,31 patients with viral meningitis and 12 patients with suppurative meningitis .Results The IFN-γlevels in cerebrospinal fluid were significantly higher in the patients with tuberculous meningitis (386 .3 ± 83 .5) ng/L than in the patients with viral meningitis (63 .4 ± 16 .2) ng/L or the patients with suppurative meningitis (116 .8 ± 22 .6) ng/L (P<0 .01) .The overlap was very small .In contrast ,the serum IFN-γ levels were very similar among the three groups (P>0 .05) .The sensitivity ,specificity and accuracy of IFN-γ level in cerebrospinal fluid for diagnosis of tuberculous meningitis were 92 .0% ,95 .3% and 94 .1% ,respectively .Conclusions Measurement of IFN-γlevel in cerebrospinal fluid may be helpful for the diagnosis of tuberculous meningitis .

Objective:To detect expression level of miR-410 in patients with systemic lupus erythematosus (SLE),and to expose the role of miR-410 and its target genes by bioinformatics methods.Methods:Expression level of miR-410 were detected by quantitative RT-PCR in peripheral blood mononuclear cells of SLE patients,and miR-410 sequence,its target genes and Genecards database were analyzed,and analysis of GO enrichment and KEGG Pathway was further performed.Results:miR-410 expression was significantly reduced in SLE patients,and its nucleotide sequence was highly conserved among species.These genes that were predicted to be regulated by miR-410 and associated with LE pathogenesis,included FASLG,CSF2,IFNAR2,MAPK1,PLCG2,IL4 and other genes.Analysis of GO enrichment revealed that miR-410's target genes were involved in cell growth,proliferation,programmed cell death,cell differentiation,immune system development and other biological activities.Analysis of KEGG Pathway showed that the target genes of miR-410 were significantly enriched in a series of signaling pathways including pathways in cancer,cytokine-cytokine receptor interaction,glioma,melanoma,TGF-β and JAK-STAT signaling pathway.Conclusion:miR-410 maybe directly regulate its target molecules,mediate various signal pathway networks,thus participate in the occurrence and development of SLE.

AlM:To investigate the effect of peripheral vision control technology for delaying the development of juvenile myopia.METHODS:A total of ninty-nine cases of 12 ~18 year-old myopic patients were randomly divided into two groups. The experimental group was peripheral vision control technology group whose members wore the special lenses which can help correct the hypermetropic defocus of peripheral retina. The other was control group whose members wore ordinary monofocal lenses. All the subjects needed to accept re-examination every 3mo and be recorded the data of dioptre, corneal curvature and axial length.RESULTS: After 18mo, the dioptre and axial length of two groups had increased in varying degree. But the data's different quantity of the corneal curvature in each group had no statistical significance ( P > 0. 05 ). Themyopia deepen quantity in experimental group was-0. 65±0. 65D and its axial growth was 0. 23±0. 22mm, and the myopia deepen quantity in control group was -1. 17 ± 0. 50D and its axial growth was 0. 41 ± 0. 17mm. Under the circumstance of valid data, the increment of myopia and axial length in experimental group was lower than that in control group (P<0. 05).CONCLUSlON: Peripheral vision control technology can delay the development of juvenile myopia effectively.

Objective To explore the clinical significance of combined detection of carbohydrate antigen(CA)125,CA153,carcinoembryonic antigen(CEA) and tumor specific growth factor(TSGF) in diagnosis for breast cancer.Methods A total of 125 patients with breast cancer were recruited as objects in this study from march 2015 to march 2016,65 patients in breast cancer group,60 patients in benign breast disease group,meanwhile 55 healthy person were enrolled in the control group.Serum tumor markers such as CA125,CA153,CEA and TSGF were detected and compared in the three groups.Results The serum CA125,CA153,CEA and TSGF levels in the breast cancer group were significant higher than those of benign breast disease group and healthy group,the differences were statistical significant(P<0.05).At the same time,the sensitivity and specificity of joint detection of four kinds of serum tumor marker were 90.2% and 88.9%,which were higher than those of single serum tumor marker detection(χ2=26.12,P<0.05).Conclusion The four kinds of serum tumor markers combined testing not only increases the sensitivity of breast cancer diagnosis,but also improved the specificity of diagnosis of breast cancer.

OBJECTIVE:To compare the cost-effectiveness of4antibacterials in the prevention of post-operative in-fection in patients with uterine-incision delivery(UID).METHODS:A total of1066cases with UID were ascribed to receive cefoperazone sodium/sulbactam sodium+arilin(therapy A),ampicillin sodium/sulbactam sodium+arilin(B),cefradine+ar-ilin(C)and cefoperazone sodium/sulbactam sodium(D),respectively for the prevention of infection.The cost-effectiveness of the4groups was analyzed retrospectively.RESULTS:The costs of the4schemes were4954.13yuan,4752.77yuan,3870.16yuan and3988.22yuan,respectively;The effective rates were98.5%,87.4%,84.6%and96.8%,respectively;The cost-effectiveness ratios were50.30,54.38,45.75and41.20,respectively;The incremental cost-effectiveness ratios of scheme A,B and D were77.98,315.22and9.68,respectively as against scheme C.CONCLUSIONS:Scheme D is more economical and reasonable in the prevention of post-operative infection in patients with UID.

Objective:To study the relationship between an interpersonal climate of universities and teachers' mental health by self-made Scale of Interpersonal Climate (SIC).Methods:532 teachers from 16 universities all over the country were randomly chosen and assessed with SRHMS and SIC.Results: ①The better interpersonal climates of universities covered around 68.8% ; the most harmonious interpersonal relationships covered 72.4% ; worse interpersonal attitude, worse interpersonal interaction and worse interpersonal distance covered 44.5%,44.5% and 46.1% respectively ; the general feelings towards mental health of teachers in universities were better (the bad self-assessment、the medium self-assessment and better self-assessment are 9.0%,56.1% and 34.9% respectively ) . ② There was prominent positive correlation between universities' interpersonal climate and teachers' mental health(r=0.44). Meanwhile there was prominent negative correlation between negative emotion and interpersonal interaction, interpersonal harmony and interpersonal attitude(r=-0.25,-0.30,-0.30), but there was prominent positive correlation between negative emotion and interpersonal distance(r=0.24). ③ There was very distinct regression between interpersonal interaction and mental health, interpersonal harmony and mental health(?=0.28、0.22).Conclusions: The general feelings towards mental health of teachers in universities are better;There are close relationships between interpersonal climates of universities and teachers' mental health, and interpersonal interaction and interpersonal harmony play a brilliant role in mental health regression , they are the positive predictable factors to teachers' mental health.

Objective To compare the effects of interferon alfa-1b(IFN?-1b) and pegylated IFN?-1b(PEG IFN?-1b) on inhibiting growth of human liver cancer cells in vivo.Methods Human liver cancer cells(HepG-2,2?l06/0.2ml) cultured in log phase were subcutaneously injected into the right rear flank of male BALB/C nude mice to establish the tumor model.After HepG-2 injection,the tumor growth was followed up for 14 days.The mice with HepG-2 tumor were randomly divided into placebo(n=7),PEG IFN?-1b(n=8) and IFN?-1b(n=8) groups.Mice received injection of placebo 0.2ml/mouse each time(three times a week),PEG IFN?-1b 150?g/mouse per injection(once a week) and IFN?-1b 50?g/mouse each time(three times a week),respectively,according to their grouping,and this regime lasted for five weeks.In order to evaluate anti-tumor activity,animals' body weight was measured every week,and the measurements of tumor volume and animals' body weight were continuously done till the mice were sacrificed at the end of the fifth week.Results PEG IFN?-1b and IFN?-1b induced a significant decrease in tumor volume and the animals' weight,the suppression rates onto tumor growth were 56%(2.190g vs.4.979g) and 42%(2.678g vs.4.979g),respectively.However,no significant difference was found in animals' weight among the three groups.Conclusion HepG-2(2?l06/0.2ml) injection into nude mice may induce a typical tumor model.PEG IFN?-1b(150?g/mouse per time,once a week) could significantly inhibit the growth of HepG-2 in vivo,and so does the IFN?-1b(50?g/mouse each time,three times per week).

Objective To investigate the clinical value of helical CT dual-phase scan in diagnosis and staging in gastrointestinal tumor.Methods Helical CT dual-phase enhanced imaging features of gastrointestinal tumor proved by surgery and pathology in 69 cases were retrospectively reviewed.Results(1)The tumors in 55 cases were enhanced ovviously in the venous phase compared with that in arterial phase(P

0.05). In HiLo group, the levels of MDA and SOD did not changed signific antly(P