1.Antimicrobial, free radical scavenging and cytotoxic activities of Khaya grandifoliola C.DC extracts
Opawale Benjamin Oyewale ; Onifade Anthony Kayode ; Ogundare Ayodele Oluyemisi
Malaysian Journal of Microbiology 2016;12(3):233-238
Aims: Khaya grandifoliola C.DC is a plant used locally in Nigeria ethno medicine for remedy of various disease
conditions. However, there is little scientific evidence to support the therapeutic claims of the plant. Therefore, these
investigations were conducted to determine the antimicrobial activity, antioxidant and cytotoxic potentials of the plant
extracts.
Methodology and results: In vitro antimicrobial activity of the leaf and stem bark extracts of K. grandifoliola against
some human pathogens was done using agar diffusion method. The free radical scavenging activity and cytotoxic
property of the plant materials were evaluated using 2, 2- diphenyl-1-pieryhydrazyl radical (DPPH) and brine shrimp
lethality bioassay methods respectively. The yields of the plant material extracts ranged from 3.57±0.06 to 6.49±0.01%
and 4.76±0.02 to 9.17±0.06% for the leaf and stem bark extracts respectively. The minimum inhibitory concentration
(MIC) of KG-A and KG-E ranged from 2.5 to 200 mg/mL and recorded remarkable activity against the growth of
Staphylococcus aureus, Escherichia coli, Salmonella typhi, Pseudomonas aeruginosa, Trichophyton rubrum and
Aspergillus flavus. However, Strepcoccus pneumoniae, Staphylococcus epidermidis, Klebsiella pneumoniae, and T.
rubrum were resistant to the KG-W. The plant extracts demonstrated high DPPH free radical scavenging activity when
compared with ascorbic acid used as control in the assay and, also exhibited lethality against brine shrimp larvae with
LC50 values ranging from: leaf extracts (0.67 to 1502 ppm) and stem bark extracts (0.91 to 1431 ppm).
Conclusion, significance and impact of study: The results show that the KG-A and KG-E have great potentials as
antimicrobial agent and may be used in the treatment of infectious diseases caused by the susceptible organisms.
Plants, Medicinal
2.Purification, Characterization and Application of Polygalacturonase from Aspergillus niger CSTRF
Arotupin Daniel Juwon ; Akinyosoye F. A. ; Onifade Anthony Kayode
Malaysian Journal of Microbiology 2012;8(3):175-183
Aims: The research was carried out to study the purification, characterization and application of polygalacturonase from Aspergillus niger CSTRF.
Methodology and Results: The polygalacturonase (PG) from the fungus was purified by ammonium sulphate
precipitation and dialysed. The resulting fraction of the enzyme was further separated by molecular exclusion and ion
exchange chromatography. The enzyme was purified 28.19 fold with a yield of approximately 69 % following purification with SP C-50. It has a relative molecular weight of 79,430 daltons and markedly influenced by temperature, pH and substrate concentrations of reactions with optimum activity at 35 °C, pH 4.0 and 8 mg/mL respectively. The PG was heat stable over a broad range of temperatures. Line weaver-Burk plot for the apparent hydrolysis of pectin showed approximately Km value of 2.7 mg/mL. The activity of the enzyme was enhanced by Na+, Ca2+, Mg2+ and Zn2+, while EDTA, PbCl2, HgCl2 and IAA were inhibitory. The ability of the purified enzyme to clarify fruit juice was also investigated.
Conclusion, significance and impact of the study: This study revealed that polygalacturonase possesses properties
for clarification of fruit juice and by extension bioprocessing applications.