1.Prevalence of Clostridium difficile toxin in diarhoeal stool samples of patients from a tertiary hospital in North Eastern Penisular Malaysia
Siti Asma' Hassan ; Norlela Othman ; Fauziah Mohd Idris ; Zaidah Abdul Rahman ; Nurahan Maning ; Rosliza Abdul Rahman ; Chan Guan Tiong
The Medical Journal of Malaysia 2012;67(4):402-405
This study describes the prevalence of Clostridium difficile toxin (CDT) in loose stool samples from inpatients aged more than two years of a tertiary hospital. A total of 175 samples that had been examined were from stool samples that were sent to the Medical Microbiology & Parasitology Laboratory for various clinical indications. The toxin was detected by a commercial immunochromatograhic test, and
the patients’ demography, clinical features, treatment and
outcomes were analyzed from their medical records.
Clostridium difficile toxin was positive in 24 (13.7%) of the stool samples. Male and female were 11 (45.8 %) and 13
(54.2 %) respectively, with the majority of them aged more
than 50 years. Most were from medical wards (n=21, 87.5%),
with the rest from surgical wards (n=2, 8.3%) and intensive
care units (n=1, 3.4%). All the CDT positive patients had
history of prior antibiotic usage within 6 weeks before the
detection of the toxin. The mean duration of antibiotics
usage was 17.75 (±13.75) days, while the mean duration of
diarrhea was 5.21((± 5.85) days. Eighteen patients had
underlying medical illnesses that were diabetes mellitus,
chronic renal disease, hypertension, ischaemic heart
disease, cerebrovascular disease and malignancy; with
seven of them being CDT positive while on chemotherapy.
Stool occult blood test was positive in 15 patients whereas
presence of pus cells in the CD positive stool samples were
detected in 21 patients. The duration of hospitalization
among the patients was 27.96 (± 23.22) days.
2.The Significant Association between Polymicrobial Diabetic Foot Infection and Its Severity and Outcomes
Sharifah Aisyah Syed Hitam ; Siti Asma& ; rsquo ; Hassan ; Nurahan Maning
Malaysian Journal of Medical Sciences 2019;26(1):107-114
Background: Foot infection is a major complication of diabetes mellitus (DM) and
its agents are usually polymicrobial. This study aims to describe the agent and determine the
association between polymicrobial infections and the severity of diabetic foot infections (DFI) and
their outcomes.
Methods: This retrospective cohort study was conducted during one year and it involved
104 patients. Their records were reviewed and assessed. The causative agents and its sensitivity
pattern were noted. The results were presented as descriptive statistic and analysed.
Results: A total of 133 microorganisms were isolated with 1.28 microorganisms per lesion.
The microorganism isolated were 62% (n = 83) GN (Gram-negative) and 38% (n = 50) GP (Grampositive).
GN microorganisms include Pseudomonas spp (28%), Proteus spp (11%), Klebsiella spp
(8%) and E. coli (4%). Staphylococcus aureus (54%) was predominant among GP, followed by
Group B Streptococci (26%) and Enterococcus spp (6%). Thirty patients (28.8%) had polymicrobial
infections. The association between the quantity of microorganisms and severity of DFI was
significant. Among severe DFI cases, 77.8% with polymicrobial microorganisms underwent
amputation compared to 33.3% with monomicrobial infection.
Conclusion: GN microorganisms were predominantly isolated from DFIs and remained
sensitive to widely used agents. Polymicrobial infections were associated with DFI severity.
3.Epidemiology and Determinants of Serologically Diagnosed HIV-1 and HIV-1&2 in Tertiary Hospitals of Eastern Peninsular Malaysia
Siti Aishah Muhadi ; Habsah Hasan ; Nurahan Maning ; Nik Rosmawati Nik Husain ; Fatimah Haslina Abdullah
Malaysian Journal of Medicine and Health Sciences 2020;16(SP 1, September):9-15
Introduction: HIV is the leading cause of mortality and morbidity worldwide. There are two types of HIV, HIV-1, and HIV-2, which are geographically different in epidemiology and determinants.
Objective: To determine the epidemiology and determinants of HIV-1 and HIV-1&2 in the three tertiary
hospitals of Eastern Peninsular Malaysia. Method: A cross-sectional study of confirmed serologically
HIV-1 and HIV-1&2 from January 2016 until December 2018. SPSS analysed all collected
data, descriptive statistics for sociodemographic data and Pearson chi-square for the association
between type of HIV with HCV, HBV, syphilis, and tuberculosis. In identifying the risk factor associated with HIV-1&2, several variables were tested by the Multiple Logistic Regression Model.
A P-value of <0.05 was considered statistically significant. Results: Out of 519 serologically diagnosed
HIV, 344 (66.28%) were HIV-1, and 175 (33.72%) were HIV-1&2. HIV positive were highly distributed
in Malay male in both groups. Most HIV-1 patients were single, unemployed, and presented with tuberculosis.
HIV-1&2 were single and employed, mostly asymptomatic at diagnosis. The commonest mode of
transmission for HIV-1 was by sexual contact (31.87%), whereas IVDU (13.63%) in HIV-1&2.
Co-infection with tuberculosis (P=0.005) and HCV (P<0.001) were significantly higher in HIV-1 as
compared to HIV-1&2. IVDU was a significant determinant to develop HIV-1&2
(Adjusted OR: 3.5, 95% CI=1.875-5.227, P<0.001). Conclusion: HIV-1&2 was high in this study.
Patients with HIV-1&2 present in less severe symptoms compared to the patient with HIV-1. A further molecular
diagnostic study should be tested to confirm the type of HIV.