Urine tests, which are performed routinely as specimens can be collected without inflicting pain on patients at all, give plenty of information about health conditions quickly thanks to the recent advances in techniques for urinalysis. Nonetheless, the constituents of urine are liable to change, and so prompt examination is necessary. In the case of inpatients, it sometimes happens that examinations begin several hours after specimen collection. Behind this, there is no time frame set for collecting urine specimens which makes it difficult to deliver specimens to the examination room without delay.　　In this study, we investigated longitudinal changes in the contents of urine specimens voided by inpatients under some specific conditions. Furthermore, we examined the effects of those intestinal bacteria (E.coli, Proteus), which are often detected in urine, on glycosuria, and the changes in nitrite reduction reaction.　　Our findings showed that urine specimens containing such physiological and pathological factors as to influence urine tests were particularly subject to change with the passage of time. Therefore, when there is a need to preserve the specimens, chilled storage using a lidded container is required. It was slso found that between E -coli and Proteus in the urine a considerable difference was noted in time-dependent change of glycolysis and nitrite reduction reaction.