Objective:In order to obtain more effective endoscopic treatment,we analysed 32 cases data of therapeutic ERCP.Methods:Of which 23 were males,9 females with a median age of 56 years.Therapeutic effort was executed in all cases including biliary drainage (n=16)，endoscopic sphincterotomy (EST) (n=15,10 of them received extraction)and extract ascaris(n=1).The discussion focus on the treatment of biliary disease.Results:All common bile-duct stone were cleared up.Satisfactory treatment was achieved in almost all with no severe side effect.Conclusions:Therapeutic ERCP is an effective method for cholangiopancreatic diseases.It is of considerable value and should be promoted in clinical application.

ObjectiveTo study the role and clinical significance of serum visfatin and retinol binding protein 4 (RBP4) in idiopathic sudden sensorineural hearing loss by measuring the change of their levels in the patients with idiopathic sudden sensorineural hearing loss.MethodsThe levels of visfatin and RBP4 were determined by ELISA method in the 102 idiopathic sudden sensorineural hearing loss patients who were observed at two different time points ( before and after treatment),and thirty-five patients with other neurologic diseases (20 with sciatica,16 with trigeminal neuralgia) and thirty healthy people were used as control.ResultsThe levels of visfatin and RBP4 in the serum of patients with idiopathic sudden sensorineural hearing loss after treatment [Visfatin (24.26 ± 2.17 ) μg/L; RBP4 (46.65± 5.26 ) mg/L]were markedly higher than the group with other neurologic diseases [Visfatin ( 20.67 ± 2.14 ) μ g/L; RBP4(34.37 ±5.73)mg/L] and the healthy control group[Visfatin(17.61 ±2.45) μg/L; RBP4 (24.82 ±5.24)mg/L] ( t =10.38,10.41,12.16,15.06,P ＜0.01),and it was significantly less than that before treatment [Visfatin(32.24 ± 2.37) μ /L; RBP4 ( 57.43 ± 6.19 ) mg/L] ( t =17.25,15.12,P ＜ 0.01 ).The levels visfatin and RBP4 in serum of severe group with idiopathic sudden sensorineural hearing loss [Visfatin ( 36.52 ± 2.46 ) μg/L; RBP4 (67.17 ± 5.92 ) mg/L] were markedly higher than those in the moderate group[Visfatin(28.92 ±2.26)μg/L; RBP4 (55.34±5.95)mg/L]( t =11.21,11.17,P ＜0.01).The levels visfatin and RBP4 in serum of moderate group were markedly higher than those in the mild group [Visfatin ( 25.31 ± 2.32 ) μg/L; RBP4 ( 47.48 ± 5.82 ) mg/L],all these differences were statistically significant( t =10.43,10.49,P ＜0.01 ).There was a positive correlation between visfatin and RBP4 in patients with idiopathic sudden sensorineural hearing loss ( r =0.68,P ＜ 0.01 ).ConclusionsThe levels of serum visfatin and RBP4 have instructive significance in idiopathic sudden sensorineural hearing loss treatingand prognosis estimating

BACKGROUND:Under the pathological conditions of urethral defect,stricture and diverticulum induce by trauma,congenital malformation,tumor and operation,autogenous tissue are used for defect repair.OBJECTIVE:To verify the feasibility of urethra constructed by tissue engineering.DESIGN,TIME AND SETTING:An observational experiment was performed at Linbaixin Medical Research Center,Sun Yat-sen University,from January 2005 to January 2006.MATERIALS:Ten New Zealand rabbits weighing 3.0-3.5 kg were used to prepare acellular urethras.Twenty BALB/cA-nude mice weighing 18-20 g,4-6 weeks old,were cultured with extracellular matrix materials in vivo.METHODS:The whole urethra harvested from New Zealand rabbit prepared the extracellular matrix materials by acellular treatment.Half a glans harvested from adult New Zealand rabbit was used for smooth muscle cells proliferation by tissue explant.The corpus spongiosum smooth muscle cells were implanted in acellular matrix materials.Then,they were cultured by subcutaneous embedding in nude mice.There were two groups in the experiment:experimental and control.The complexs of corpus spongiosum smooth muscle cells implanted in acellular matrix materials were embedded in the experimental group,while the simple acellular matrix materials were embedded in the control group.MAIN OUTCOME MEASURES:After 1,2,4,6,8 weeks of embedment,the growth of the complexs were examined by gross,histologic and electron microscopic observation.The expression of smooth muscle cells were identified by immunohistochemistry method.RESULTS:①Under the gross observation,incisions on the back of the nude mice healed well,the diets and activities of the nude mice were normal.In the experimental group,there were thin tissue membranes on the surface of the acellular matrix materials at 1-2 weeks after the embedment.The thickness of tissue membrane increased gradually and there were small blood vessels grown at 4 weeks.The new cells were instead of acellular matrix materials at 6-8 weeks.②Under HE straining,the acellular matrix materials were absorbed.Various cells,most of them were smooth muscle cells,would be instead of the acellular matrix materials.And unequal blood vessels grown in matrix materials,most of them had the trend of blood sinus formation.These results suggested that the complexs formed the structure similar to normal urethra in the nude mice.③Under the electron microscopic observation,there were blood vessels grown in the matrix materials from 4 weeks after embedment.More smooth muscle cells,endothelial cells,and little fibroblast cells,macrophages were observed,as well as some apoptotic smooth muscle cells.④After immunohistochemical staining of ?-smooth-muscle actin antibody,the growth of smooth muscle cells could be observed.CONCLUSION:The corpus cavernosum tissue similar to normal urethra can be constructed by using tissue-engineering technique.

Objective To study the changes in echocardiography in normotensive patients with type2 diabetes mellitus (T2DM) in order to establish early diagnosis and give preventive measures for diabetic cardiomyopathy (DCM). Methods Microvasculopathy was diagnosed in T2DM patients when microalbuminuria exceeded 15ml/min. Fifty-four T2DM patients were divided into two groups: group A (patients without microvasculopathy, n=32 ), group B (patients with microvasculopathy, n=22). All of T2DM were submitted to echocardiography and the results were compared with healthy people of matched sex and age (group C, n=20). Results There were no differences in systolic function parameters among the groups. Left ventricular mass index (LVMI) was higher in two groups of diabetics compared with group C. Diastolic dysfunction of various degrees was found in the two groups of diabetics, regardless of the presence of microvasculopathy, with a manifestation of a decrease of blood flow velocity in early diastolic phase and increase in late diastolic phase (P

Objective To investigate the effect of cold pressed Camelina Sativa oil (CPCO) on growth performance and lipid metabolism of hyperlipemia rats. Method The rats were divided into control group (Cont),high fat diet group (HFD),fish oil group (HFD+FO),low dose CPCO group (HFD+LCPCO) and high dose CPCO group (HFD+HCPCO). The latter 4 groups were fed high fat feed. Fo and CPCO were given by gavage. Total cholesterol (TC),triglyceride (TG),high density lipoprotein cholesterol (HDL-C) and HDL-C subtype levels in serum were determined at the end of 6w. The distribution of HDL-C particles was studied by agarose gel electrophoresis. Results HFD resulted typical hyperlipemia. Body weight,TC and TG level were significantly decreased,and HDL-C particles tended to be miniaturized and the HDL3 level increased by feeding Camelina Sativa oil as compared with HFD group. TC content in HFD+FO and HFD+HCPCO group was decreased 46.06% and 30.60% respectively. TG content in these two groups was decreased 25.81% and 22.72% respectively as compared with HFD group. Conclusion Chinese Camelina Sativa oil which contained high level of ?-linolenic acid(ALA)could prevent and cure hyperlipidemia by regulating blood lipid metabolism and protecting hepatic function.

Objective To research the expression of Neuritin in gastric cancer .and the relationship between the expression of Neuritin with the occurrence and development of gastric cancer .Methods Collected 58 surgical specimens of gastric cancer from 2010 to 2013 in the first affiliated hospital of Shihezi University ,immunohistochemistry was used to examine the expression of Neu-ritin in gastric cancer and normal tissues near the cancer of the stomach .Results Immunohistochemical staining showed that Neu-ritin was moderately or highly expressed in 96 .55% (56/58) of gastric cancer ,and Neuritin was moderately or highly expressed in 94 .83% (55/58) of normal tissues near the cancer .There was no statistically significantly difference between two groups (P>0 .05);Neuritin was highly expressed in 82 .76% (48/58) of gastric cancer ,and Neuritin was highly expressed in 15 .52% (9/58) of normal tissues near the cancer ,differences between the two groups had statistically significantly (P<0 .05) .Expression of Neuritin was not correlated with gender ,age ,TNM stage ,infiltration depth ,lymph node metastasis ,differentiated degree ,the pathologic type or distant metastasis(P> 0 .05) .Conclusion The Neuritin expression level between gastric cancer and normal tissues near the cancer exist differences .There is overexpression of neuritin in gastric cancer .

Objective To study the role of endothelial cells on the inflammatory cytokine release in septic shock through the septic shock serum stimulating human primary endothelial cells (HPAEC) and peripheral blood mononuclear cells(PBMC).Methods PBMC isolated from healthy people by density gradient centrifugation.HPAEC cell surface markers CD144 and von Willebrand factor(vWF) molecule expression by RT-PCR and Western blot.Serum levels of IL-6,TNF-α,MCP-1 from septic shock patients and healthy human detected by ELISA.HPAEC and PBMC were stimulated with the isolated serums and LPS,respectively.ELISA was used to detect the supernatant IL-6,TNF-α,MCP-1 levels.HPAEC membrane molecules ICAM-1 expression was detected by flow cytometry with serum shock and LPS stimulation.Supernatant levels of IL-6,TNF-α,MCP-1 of HPAEC with S1P1 receptor agonist CYM-5442 pretreatment was detected by ELISA after shock serum stimulation.Results Endothelial cell markers CD144 and vWF molecules could be detected in the HPAEC.Levels of inflammatory cytokines IL-6,TNF-α,MCP-1 in patients with septic shock serum were significantly higher than healthy people (P＜0.01).PBMC and HPAEC with LPS or shock serum treatment respectively,compared with normal group,levels of inflammatory cytokines in the culture supernatant were significantly higher(P＜0.01).For PBMC,the level of inflammatory cytokines between shock group and LPS group were not significantly different (P＞0.05).But for HPAEC,levels of inflammatory cytokines in the supernatant of the shock group compared to the LPS group was significantly higher (P＜0.01).Similarly,when two cells after LPS stimulation,IL-6,TNF-α levels of HPAEC's supernatant were significantly lower than PBMC' s (P＜0.01),MCP-1 levels was no difference (P＞ 0.05).But when the stimulation of shock serum,HPAEC of IL-6,TNF-α levels and PBMC no significant difference (P ＞0.05).MCP-1 was significantly increased (P＜0.01).Shock patients serum stimulation S1P1 receptorspecific agonist CYM-5442 pretreatment of HPAEC with pretreatment of S1P1 receptor specific agonist CYM-5442,the culture supernatant of inflammatory cytokines IL-6,TNF-α,MCP-1 levels were significantly lower (P＜0.01).Conclusion Endothelial cells may play a central role on the release of inflammatory cytokine during septic shock.

Objective: To establish a mini invasive way to evaluate endothelium function. Methods: Group 1 consisted of 10 subjects with no risk factors for coronary artery disease, group 2 consisted of 11 subjects with risk factors for coronary artery disease and angiographically normal coronary arteries. Two groups of patients underwent transesophageal echocardiography(TEE) for assessment of LAD diastolic blood flow velocity (V) and time velocity integral (VTI) at rest (B 1), 2 min after immersion of the hand in cold water (CPT), 5 min after rewarming of the extremity (B 2), and 5 min after administered nitroglycerin. The percent change in V and VTI were calculated between B 1 and CTP and B 2 and nitroglycerin. Results: After CPT the percent increase in V and VTI were significant in group 1 than group 2 ( P

A total of 107 vitiligo patients were randomly divided into 3 groups.Group A received an intralesional injection of Bacillus Calmette-Guérin-polysaccharide nucleic acid (BCG-PSN) (n =34),group B Compound Glycyrrhizin Tablets (n =36) and group C both (n =37).Before treatment and 3 months after treatment,cellular immune function was detected for each group.Paired comparisons of 3 groups before and after treatment showed that CD4 +,CD4 +/CD8 + ratio increased (all P ＜ 0.05) and CD8 + decreased (P ＜0.05).After treatment,as compared with groups A and B,CD4 + increases (both P ＜ 0.05) and CD8 + decreased in group C (P ＜0.05).Group C had an efficiency rate of 91.9％ and it was higher than the other two groups (both P ＜ 0.05).An intralesional injection of BCG-PSN plus Compound Glycyrrhizin Tablets could improve immune function and treat vitiligo patients efficiently.

Objective To explore the clinical effect of creatine phosphate on infantile with rotaviral enteritis and myocardial damage.Methods One hundred and twenty children with rotaviral enteritis and myocardial damage were randomly divided into the control group (n =60) and observation group (n =60).Two groups were given antiviral,rehydration,nutritional support,redressing acid-base imbalance and electrolyte disorders and other conventional treatment.The patients of the control group were given 160 mg/(kg · d) 1,6Fructose Diphosphate(1 times/d) on the basis of routine treatment,while those in the observation group were given 1.0 g creatine phosphate + 150 ml 5％ glucose injection(1 times/d) on the basis of routine treatment,both courses of two groups were 7 days.The levels of myocardial enzymes were measured and the clinical effect and adverse reactions were recorded.Results The total effective rate in observation group was 95.0％ (57/60),obviously higher than that of control group (80.0％ (48/60)) and the differences were statistically significant (x2 =6.268,P ＜ 0.05).There were no statistical significant difference in terms of myocardial enzyme between the two groups before treatment(P ＞0.05).The level of lactate dehydmgenase(LDH),creatine kinase (CK),creatine kinase isoenzyme (CK-MB) and aspartate aminotransferase (AST) of observation group were (163.8 ±18.5) U/L,(152.8±26.4) U/L,(19.4±7.8) U/Land (24.4 ±6.8) U/Lafter treatment,and (193.4 ± 22.8) U/L,(168.6 ± 32.6) U/L,(27.4 ± 9.2) U/L and (35.4 ± 9.8) U/L of control group,and the differences between the two groups were significant (t =3.484,3.228,4.278 and 4.729; P ＜ 0.05).There was no obvious adverse reaction during the treatment course.Conclusion The clinical effect of creatine phosphate on infantile with rotaviral enteritis and myocardial damage is remarkable and safe,and it is worth of clinical application.