Objective To develop a conductance-ion chromatography for simultaneous determination of 5 anions in drinking water. Methods The orthogonal experiment method L9(34) was used to select the optimum working conditions of IC-2000 ion chromatography. Under the founded chromatography condition, 5 anions in water samples were determined simultaneously after filtered with 0.45 ?m filter membrane. Results Both of the precision and accuracy of this method were desirable, all the relative standard deviation was less than 5.0%, the rates of recovery were 90%-106.7%, the correlation coefficient was more than 0.999 except NO2-(0.990 5). Conclusion This method is simple, accurate, quick and suitable for simultaneous determination of 5 anions in drinking water with no need of sample pretreatment.

OBJECTIVETo investigate the effect of six cold traditional Chinese medicine on the energy metabolism factors in rats skeletal muscle.

METHODThe activity of Na(+)-K(+) -ATPase, Ca(2+) -ATPase and succinate dehydrogenase (SDH), the content of muscle glycogen, and the mRNA expression of skeletal muscle uncoupling protein 3 (UCP3) were measured after rats having been administrated with water extracts of Radix Sophorae Flavescentis, Fructus Gardeniae, Cortex Phellodendri, Radix Scutellariae, Rhizoma Coptidis, and Radix Gentianae respectively at the dose of 6.0, 7.0, 8.4, 6.0, 7.0, 4.0 g x kg(-1) for 30 days.

RESULTThe activity of Na(+) -K(+) -ATPase has been depressed significantly and the content of skeletal muscle glycogen has been increased remarkably by six cold traditional Chinese medicine. The decreased tendency has been found on activity of Ca(2+) -ATPase and SDH, only the Radix scutellariaeg group decreased the Ca(2+) -ATPase activity significantly (P < 0.05), the SDH activity was decreased high significantly by Radix scutellariae, Cortex Phellodendri, Radix Gentianae and significantly by Rhizoma Coptidis. The mRNA expression of UCP3 has been decreased high significantly by all five cold traditional Chinese medicine except Cortex Phellodendri group with the decreased tendency of UCP3 mRNA expression.

CONCLUSIONThe cold traditional Chinese medicine has the significant effects on the skeletal muscle energy metabolism by decreasing the utlization of the glucose and the activity of mitochondria SDH to reduce the production of ATP, and depressing the activity of Na(+)-K(+) -ATPase and Ca(2+) -ATPase to cut down the consumption of ATP, by decreasing the mRNA expression of UCP3 to decrease the heat production.

Animals ; Drugs, Chinese Herbal ; pharmacology ; Energy Metabolism ; drug effects ; Male ; Muscle, Skeletal ; drug effects ; metabolism ; Plants, Medicinal ; chemistry ; Random Allocation ; Rats ; Rats, Sprague-Dawley

Objective:To explore the mediating effect of breastfeeding self-regulation on the relationship between breastfeeding self-efficacy and breastfeeding adaptation in parturient women.Methods:Totally 310 parturient women who visited the postpartum clinic at Wuxi Maternity and Child Health Care Hospital from May to September 2023 were selected by convenience sampling. The participants were surveyed using a General Information Questionnaire, the Breastfeeding Self-Regulation Questionnaire (BSRQ), the Breastfeeding Self-Efficacy Scale Short Form (BSES-SF), and the Breastfeeding Adaptation Scale (BFAS). The mediating effect of breastfeeding self-regulation between breastfeeding self-efficacy and breastfeeding adaptation was tested using the Process macro and Bootstrap method.Results:The scores for the 310 parturient women were 16.83 (13.83, 19.67) for BSRQ, (45.20±12.38) for BSES-SF, and 107.50 (96.00, 118.00) for BFAS. Spearman correlation analysis showed that breastfeeding self-efficacy was positively correlated with breastfeeding self-regulation and breastfeeding adaptation ( rs=0.314, 0.673; P<0.01), and breastfeeding self-regulation was positively correlated with breastfeeding adaptation ( rs=0.618; P<0.01). The mediating effect of breastfeeding self-regulation between breastfeeding self-efficacy and breastfeeding adaptation was 0.149, accounting for 21.88% of the total effect. Conclusions:Breastfeeding self-regulation mediates the relationship between breastfeeding self-efficacy and breastfeeding adaptation in parturient women. In early postpartum breastfeeding management, healthcare professionals should take measures to improve breastfeeding self-efficacy, help parturient women enhance their self-regulation abilities, and thereby improve their breastfeeding adaptation.