1.Development trends in information resources organization under E-Learning environment
Yanling LUO ; Hua LI ; Ningxia YUN
Chinese Journal of Medical Library and Information Science 2014;(9):32-34,61
The importance of information resources organization under E-Learning environment was analyzed ac-cording to the new features and technologies of E-Learning environment with the development trends in information resources organization summarized .
2. Antiasthma biological potency applied for Ephadra Herba quality evaluation
Chinese Traditional and Herbal Drugs 2015;46(24):3695-3703
Objective: To study the anti-asthma action and biological potency differences of Ephedra Herba from 37 different habitats. Methods: Taking the isolated tracheal smooth muscle from guinea pig to establish antiasthma pharmacological model, the ephedra decoction at different concentration was added to the sink by cumulative metrology method, antispasmodic percentage and biological potency value of Ephedra Herba from different habitats were calculated and the cluster analysis for the biological potency value of Ephedra Herba from different habitats was conducted. Results: Ephedra Herba had the antispasmodic acction to the isolated tracheal smooth muscle of guinea pig caused by histamine phosphate, and compared with the control medicine, Ephedra Herba from 20 habitats showed significant differences (P < 0.05), and the antispasmodic acction of Ephedra Herba was in a dose-dependent manner with administered concentration, when the concentration was raised and the antispasmodic percentage increased, and there were no significant difference in the antispasmodic percentage between Ephedra sinica and E. intermedia (P > 0.05); Compared with the control medicine, the antiasthma biological potency of Ephedra Herba from 37 different habitats existed significant or very significant differences (P < 0.05, 0.01), and the antiasthma biological potency value was 22.35-489.04 U/g, while FL% was 13.15%-38.97%, which revealed that the high potency level had a difference of 21.88 times from the low one. And the antiasthma biological potency of Ephedra Herba had lowly significant correlation differences with the total determination of ephedrine and D-pseudephedrine; The Ephedra Herba with different antiasthma biological potency could be identified by cluster analysis method, in which the biological potency from 24 ones in total of 37 Ephedra Herba was higher than that in the control medicine and accounted for 64.86% of the total samples. Conclusion: The anti-asthma biological potency value of Ephedra Herba could quantitatively evaluate the quality of Ephedra Herba from different habitats.
3.Value of five scoring systems in predicting the severity of hyperlipidemic acute pancreatitis: A comparative analysis
Yuanzhen WANG ; Yaling YUN ; Juan XIE ; Liya HUANG
Journal of Clinical Hepatology 2023;39(6):1391-1397
Objective To investigate the value of pancreatitis activity scoring system (PASS) score, Bedside Index for Severity in Acute Pancreatitis (BISAP) score, Acute Physiology and Chronic Health Evaluation Ⅱ (APACHE-Ⅱ) score, harmless acute pancreatitis score (HAPS), and Ranson score in evaluating the severity and prognosis of hypertriglyceridemia acute pancreatitis (HTGAP). Methods A retrospective analysis was performed for the clinical data of 300 patients with HTGAP who were admitted to General Hospital of Ningxia Medical University from January 2016 to January 2022, and according to the disease severity, these patients were divided into mild acute pancreatitis (MAP) group, moderate-severe acute pancreatitis (MSAP) group, and severe acute pancreatitis (SAP) group. Clinical data and the above scores were compared between the three groups. The chi-square test was used for comparison of categorical data between groups; an analysis of variance was used for continuous data with homogeneity of variance, and the Kruskal-Wallis H test was used for comparison of non-normally distributed continuous data between groups. The receiver operating characteristic (ROC) curve was plotted according to disease severity, and related indicators were compared in terms of the area under the ROC curve (AUC). Results There were significant differences between the three groups in PASS score ( F =219.351, P < 0.01), Ranson score ( χ 2 =83.084, P < 0.01), APACHE-Ⅱ score ( χ 2 =43.388, P < 0.01), and BISAP score ( χ 2 = 50.785, P < 0.01). Compared with the other four scoring systems in evaluating and predicting disease severity, PASS score had the highest sensitivity of 0.945 and the largest AUC of 0.963, followed by Ranson score with a sensitivity of 0.655 and an AUC of 0.819. Conclusion For patients with HTGAP, PASS score can more accurately assess the severity and prognosis of HTGAP patients and thus holds promise for clinical application.
4. FoxO1 regulates Hcyinduced hepatocyte apoptosis through ATF6
Qing-Qing WANG ; Xin-Yan WU ; Long XU ; Hui ZHANG ; Wei GUO ; Yi-Deng JIANG ; Yun JIAO ; Xiu-Ling HAI ; Yi HAO ; Yi-Deng JIANG ; Yi-Deng JIANG
Chinese Pharmacological Bulletin 2021;37(2):203-209
: Aim To explore the role and possible mechanism of forked transcription factor (FoxO1) in hepatocyte apoptosis induced by homocysteine (Hey) . Methods The male cbs
5. Effect and Mechanisms of Lycii Fructus on Hepatoma HepG2 Cell Line-induced Cachexia in Mice
Ting LIU ; Yan DING ; Ting-ting YANG ; Xiao-na MA ; Yi-ran JIN ; Hai-bin MA ; Xue-yun LIANG
Chinese Journal of Experimental Traditional Medical Formulae 2019;25(9):89-94
Objective: To investigate the inhibitory effect and mechanism of the extracts from fresh Lycii Fructus (LBL) on hepatoma HepG2 cell-induced cachexia in mouse.Method: The human hepatoma cell line HepG2 was injected into BALB/C mice to establish the cachexia model.Then the LBL was fed to the models respectively in low dose (5 mg·kg-1) or high dose (25 mg·kg-1).After 28 days of continuous feeding,the mice's body weight was detected.The expression levels of creatine kinase (CK),interleukin-1β (IL-1β),interleukin-6(IL-6) and tumor necrosis factor-α(TNF-α) were evaluated by enzyme linked immunosorbent assay (ELISA).The muscle degradation and the expression of p38 mitogen-activated protein kinase (p38 MAPK) were detected by immunohistochemistry staining.The expression levels of phospho-p38 mitogen-activated protein kinase (p-p38 MAPK) and nuclear factor-κB (NF-κB) were determined by Western blot.Result: Compared with cachexia group,the loss of body weight and muscle decomposition were significantly inhibited both in the low dose LBL group and high dose LBL group (P<0.05,P<0.01).The level of plasma CK decreased significantly both in the low-dose LBL group and the high-dose LBL group (P<0.01).ELISA tests revealed lower expression levels of IL-1β,IL-6 and TNF-α in both the low-dose LBL group and the high-dose LBL group (P<0.05,P<0.01).Immunohistochemistry staining showed that the expression of p38 MAPK was inhibited both in the low-dose LBL group and the high-dose LBL group (P<0.05).Western blot indicated that the expressions of p-p38 MAPK and NF-κB were inhibited both in the low-dose LBL group and the high-dose LBL group (P<0.01).We found that the high-dose LBL group shows a higher inhibitory capability than the low-dose LBL group.Conclusion: LBL could inhibit the cachexia induced by hepatoma HepG2 cell line in mouse,suggesting LBL could reduce major cytokine and plasma inflammatory factors through p-p38 MAPK pathway.
6. Early and mid-term postoperative comparison of left ventricular structure and function between the mitral repair and replacement in patients with mitral regurgitation
Sen YU ; Shuming GE ; Shili DING ; Yun WANG
Chinese Journal of Thoracic and Cardiovascular Surgery 2019;35(10):583-587
Objective:
To compare the early and mid-term postoperative changes of left ventricular structure and function beteen mitral repair and replacement in patients with mitral regurgitation.
Methods:
100 patients with degenerative mitral regurgitation underwent mitral valve replacement and mitral repair from January 2008 to January 2018 were retrospectively studyed. Of them, 46 patients underwent mitral repair and(repair group) 54 patients underwent mitral valve replacement(replacement group). The results of color Doppler echocardiography before, one week after, 12 months after and 24-36 months after operation were collected. Left atrial diameter(LAD), left ventricular end diastolic diameter(LVEDD) and left ventricular end systolic diameter(LVESD) were selected to evaluate left ventricular structure, fraction shortening(FS)、left ventricular stroke volume( SV )and left ventricular ejection fraction(LVEF) to evaluate left ventricular function. The data were analyzed by SPSS 22.0.
Results:
In left ventricular structural parameters, LAD, LVEDD and LVESD in mitral repair group and replacement group were significantly improved compared with those before operation(
7. Mechanism of PSMD10 in homocysteine activating hepatocyte autophagy and promoting liver injury
Xin-Yan WU ; Qing-Qing WANG ; Long XU ; Hui ZHANG ; Wei GUO ; An-Ning YANG ; Yi-Deng JIANG ; Xin-Yan WU ; Qing-Qing WANG ; Long XU ; Hui ZHANG ; Wei GUO ; An-Ning YANG ; Yi-Deng JIANG ; Xin-Yan WU ; Qing-Qing WANG ; Long XU ; Hui ZHANG ; Wei GUO ; An-Ning YANG ; Yi-Deng JIANG ; Yun JIAO ; Yin-Ju HAO
Chinese Pharmacological Bulletin 2021;37(4):535-541
Aim To discuss the mechanism of proteasome 26S subunit non-ATPase 10(PSMD10) activating hepatocyte autophagy and promoting liver injury by in homocysteine. Methods Wild mice and cystathionine β-synthase (CBS) gene knockout mice were used and divided into normal (cbs
8.Circulating Dickkof-1 as a potential biomarker associated with the prognosis of patients with rheumatoid arthritis-associated interstitial lung disease.
Jing XUE ; Yu-Jiao WANG ; He-Chun XIA ; Xue-Yun LIANG ; Jie-Da CUI ; Min YU ; Xiao-Ming LIU ; Shu-Hong CHI ; Juan CHEN
Chinese Medical Journal 2021;134(9):1119-1121
9.Rho/ROCK signaling pathway and anti-cryodamage ability of human sperm.
Jia TIAN ; Shao-Hua ZHANG ; Ke MA ; Bao-Yun ZHAO ; Bei YAN ; Cheng-Bin PEI ; Yue ZHOU ; Hong-Hong WANG ; Hong-Yan WANG ; Liang-Hong MA ; Xin-Zong ZHANG
National Journal of Andrology 2019;25(5):322-328
Objective:
To investigate the influence of the Rho/ROCK signaling pathway on the anti-cryodamage ability of human sperm and provide some theoretical evidence for the development of high-efficiency semen cryoprotectants.
METHODS:
We collected semen samples from 25 healthy males, each divided into a fresh, a normal cryopreservation control and an Rho-inhibition group. Before and after freezing, we detected sperm motility, viability, membrane integrity, morphology, DNA fragmentation index (DFI), acrosomal enzyme activity (AEA) and mitochondrial membrane potential (MMP) and determined the expressions of RhoA and ROCK proteins in the sperm by immunofluorescence staining.
RESULTS:
Compared with the normal cryopreservation control, the frozen-thawed sperm of the Rho-inhibition group showed significantly increased sperm motility ( [51.20 ± 7.70]% vs [57.50 ± 6.83]%, P = 0.002), survival rate ( [52.87 ± 5.07]% vs [60.24 ± 5.53]%, P = 0.001), membrane integrity ([59.78±5.56]% vs [67.10 ± 4.43]%, P = 0.001), percentage of morphologically normal sperm ([4.83 ± 1.11]% vs [7.46 ± 1.28], P = 0.001) and MMP (56.30 ± 4.28 vs 63.11 ± 2.97, P = 0.001), but decreased DFI ([27.64 ± 6.64]% vs [18.87 ± 4.07]%, P = 0.001). There was no statistically significant difference in the AEA of the frozen-thawed sperm between the control and Rho-inhibition groups (97.65 ± 9.31 vs 98.30 ± 11.33, P > 0.05). Immunofluorescence staining revealed extensive expressions of RhoA and ROCK proteins in the head and neck of the sperm.
CONCLUSIONS
The Rho/ROCK signaling pathway plays a role in the cryodamage to human sperm, and inhibiting the activity of Rho/ROCK can significantly improve the ability of sperm to resist cryodamage.
10. The protective effect of miR-5088-5p on hepatocyte pyroptosis induced by homocysteine
Xiao-Yan DONG ; Da-Yue LIU ; Ling-Bo XU ; Yi-Deng JIANG ; An-Ning YANG ; Gui-Zhong LI ; Xiao-Yan DONG ; Da-Yue LIU ; Ling-Bo XU ; Ling-Yu GU ; Yi-Deng JIANG ; An-Ning YANG ; Yun JIAO ; Gui-Zhong LI ; Xiao-Yan DONG ; Da-Yue LIU ; Ling-Bo XU ; Ling-Yu GU ; Yi-Deng JIANG ; An-Ning YANG ; Yun JIAO ; Gui-Zhong LI ; Ling-Yu GU ; Yun JIAO
Chinese Pharmacological Bulletin 2021;37(11):1565-1570
Aim To explore the role of miR-5088-5p in hepatocyte pyroptosis induced by homocysteine. Methods Hepatocytes were cultured and divided into control group and Hcy group. After transfected miR-5088-5p NC and miR-5088-5p mimic under Hcy treatment, the expression of NLRP3, Caspase 1 and IL-1β was detected by Western blot. The expression of miR-5088-5p was detected by RT-qPCR. CBS