OBJECTIVE: To establish an HPLC method for the determination of plasma concentration of Irbesartan tablets in rats and its pharmacokinetics study.METHODS: The determination was performed on Cosmosil-C18 column with mobile phase consisted of phosphoric acid-methanol-acetonitrile(7 ∶ 80 ∶ 13,V/V/V) at flow rate of 1.0 mL?min-1.The detection wavelength was set at 272 nm.Rats were given Irbesartan solution 30 mg?kg-1 via i.g.gtt.The plasma concentration of irbesartan were determined before medication and at 0 min,5 min,10 min,20 min,30 min,45 min,60 min,120 min,180 min,240 min,360 min,480 min after medication.The pharmacokinetics parameters were estimated.RESULTS: The linear range of irbesartan was 0.5～50 ?g?mL-1(r=0.999 1) with an average recovery of 93.89%(RSD