Objective To study the phenolic compounds from Sagina japonica. Methods The separation and purification were carried out by silica gel Sephadex LH-20 gel and RP C-18 colomn chromatography. The structures were identified by spectra. Results Nine phenolic compounds were isolated from S. japonica. They were identified as:p-E-methoxy cinnamic acid methyl ester (Ⅰ),umbelliferone (Ⅱ),7-methoxy coumarin (Ⅲ),5,7-di-hydroxy coumarin (Ⅳ),5,7-di-methoxy coumarin (Ⅴ),cerarvensin-7-O-glucoside (Ⅵ),5,7,2'-trihydroxy-8-methoxy flavone (Ⅶ),5,7-di-hydroxy-8,2'-di-methoxy flavone (Ⅷ),5,7,3',4'-tetrahydroxy-6-methoxy flavone (Ⅸ). Conclusion All of these compounds are first isolated from S. japonica.

Objective To investigate the chemical constituents of Taxodium ascendens collected in Yunnan Province of China.Methods Column chromatography techniques were used for separation and purification of the compounds and extensive spectral analyses including 2D NMR spectrum were employed for structural elucidation.Results Fourteen compounds were isolated from the branches and leaves of T.ascendens.Structures of six compounds were identified by 13C-NMR,1H-NMR,and MS as following: tetrahydro-2,4-bis(4-hydroxyphenyl)-2H-pyran-3,5-diol(Ⅰ),3,5-bis(4-hydroxyphenyl)-4-pentene-1,2-diol(Ⅱ),3,5,7,3′,5′-pentahydroxyflavan(Ⅲ),4-(2,3-dihydro-3-hydroxymethyl-5-(3-hydroxypropyl)-7-methoxybenzofuran-2-yl)-2-methoxyphenol(Ⅳ),2-(3,4-dimethoxyphenoxy)-tetrahydro-6-(hydroxymethyl)2H-pyran-3,4,5-triol(Ⅴ),2,5-dihydro-3,5-bis(4-hydroxyphenyl)-2furanmethanol(Ⅵ).Conclusion Compound Ⅰ is a new compound named as ascenden pyrandiol and other compounds are isolated from T.ascendens for the first time.

A new phenethanol, (2'R)-4-(2', 3'-dihydroxy-3'-methyl-butanoxy)-phenethanol (1), along with other eleven known benzene derivatives (2-12) were isolated from the roots, stems and leaves of Clausena excavata (Rutaceae). Compounds 3 and 4 are new natural products, and compounds 5-8, 10-12 were isolated from C. excavata for the first time. Their structures were elucidated on the basis of MS, 1D and 2D NMR spectroscopic analyses including HSQC, COSY and HMBC experiments. 1 was tested for its cytotoxicities against A549, HeLa and BGC-823 cancer cell lines, and antimicrobial activities against Candida albicans and Staphylococcus aureus. The results showed that 1 did not exhibit cytotoxic and antimicrobial activities.

A new benzene derivative microintegerrin C (1) and a new norsesquiterpenoid microintegerrin D (2), along with six known compounds (3-8), were isolated and identified from stems and leaves of Micromelum integerrimum by various chromatographies such as silica gel, Sephadex LH-20, RP-18 column chromatography and HPLC. Their structures were mainly identified based on the spectral data analysis such as 1D-, 2D-NMR and HR-EI-MS. All known compounds were isolated from this plant for the first time.

Object To study the chemical constituents of our folk herb, Cucubalus baccifer L Methods The components were separated on Diaion HP 20 and silica gel column chromatography and the structures were identified by spectral evidence Results Six compounds from ethyl acetate extracts were elucidated as 6? methoxy piperidin 2 one (Ⅰ), pterolactam (Ⅱ), 5, 7, 4′ trihydroxyflavone (Ⅲ), 4 hydroxy 3 methoxybenzopropanyl acid (Ⅳ), 4 hydroxybenzoaldehyde (Ⅴ), and 4 hydroxybenzoic acid (Ⅵ) Conclusion Compound Ⅰ was a new naturally occurring compound and others were first isolated from this plant

Pedicularis comprises about 300 species in China and hemiparasite is its most important characteristic. Many compounds have been isolated from the genus, including phenylpropanoids, iridoids, flavonoids and so on. Among them, some showed antioxidative, antitumor, antifatigue, repairing DNA and antidiabetic activities. This review summarized hemiparasite, chemical constituents and pharmacological activities on Pedicularis plants. The aim was to provide information for the further study of Pedicularis.
Animals ; Drugs, Chinese Herbal ; chemistry ; pharmacology ; Humans ; Pedicularis ; chemistry ; Research

One new dicyclopeptide cyclo-(L-N-methyl Glu-L-N-methyl Glu) (1), together with one new natural dicyclopeptide cyclo-(L-methyl Glu ester-L-methyl Glu ester) (2), and two known dicyclopeptides cyclo-(L-methyl Glu ester-L-Glu) (3), and cyclo-(L-Glu-L-Glu) (4), were isolated from the aerial parts of Dianthus chinensis L. Their structures were determined by spectroscopic analyses and chemical methods.

OBJECTIVETo study the chemical constituents from the fruits of Leonurus japonicus.

METHODThe compounds were extracted by 70% methanol, then isolated and purified by column chromatography on silica gel, RP-18, Sephedax LH-20 and HPLC. Their structures were determined on the basis of spectroscopic data and physicochemical property.

RESULTTwo 28-noroleanane-derived spirocyclic triterpenoids including a new acylated nortriterpenoid were isolated and identified as (17R)-19(18 --> 17)-abeo-2alpha,18beta,23-trihydroxy-3beta-E-feruloyoxy-28-norolean-12-ene (1) and phlomistetraol B (2).

CONCLUSIONCompound 1 is a new compound,named as leonujaponin A. Both compounds 1-2 are isolated for the first time from Leonurus plants.

Chromatography, High Pressure Liquid ; Fruit ; chemistry ; Leonurus ; chemistry ; Triterpenes ; chemistry ; isolation & purification

OBJECTIVETo study the chemical constituents of the whole plants of Pedicularis densispica.

METHODThe chemical constituents were isolated by various chromatographic methods and their structures were determined by chemical evidences and spectral data.

RESULTTen compounds were isolated and identified as acacetin (1), apigenin-7-0-beta-glucopyranoside (2), kaempferol-3,7-O-alpha-dirhamnopyranoside (3), scutellarein-7-0-beta-glucopyranoside (4), chrysoeriol-7-O-beta-glucopyranoside (5), pedicutricone A (6), dearabinosyl pneumonanthoside (7), salidroside (8), darendoside B (9), and maltol-beta-D-glucopyranoside (10).

CONCLUSIONThese compounds were isolated from the titled plant for the first time. Except compounds 6 and 8, the others were obtained for the first time from the genus Pedicularis.

Drugs, Chinese Herbal ; analysis ; isolation & purification ; Flavonoids ; analysis ; isolation & purification ; Pedicularis ; chemistry ; Sesquiterpenes ; analysis ; isolation & purification

OBJECTIVETo study the chemical constituents in fruit of Alpinia oxyphylla and their cytotoxicities on cancer cell lines.

METHODCompounds were isolated and purified by various column chromatographic methods. Their structures were determined by physico-chemical properties and spectral analyses. Compound cytotoxicity was assessed by the sulforhodamine B (SRB) assay.

RESULTEight compounds were obtained from Me2CO-H2O (70%) extract of the fruit of A. oxyphylla and their structures were identified as: (9E)-humulene-2, 3; 6, 7-diepoxide (1), 3(12), 7(13), 9(E)-humulatriene-2, 6-diol (2), (-)-oplopanone (3), yakuchinone A (4), yakuchinone B (5), tectochrysin (6), isovanillin (7), (2E, 4E)-6-hydroxy-2, 6-dimethylhepta-2, 4-dienal (8), and the cytotoxicities of compounds 1, 3-5 on cancer cell lines, A549, HT-29 and SGC-7901, were also investigated.

CONCLUSIONCompounds 1-3, 7, 8 were isolated for the first time from this genus and compounds 1, 3-5 exhibited no cytotoxicity against three cancer cell lines at a concentration of 10 mg x L(-1).

Alpinia ; chemistry ; Benzaldehydes ; chemistry ; isolation & purification ; pharmacology ; Cell Line, Tumor ; Cell Survival ; drug effects ; Diarylheptanoids ; chemistry ; isolation & purification ; pharmacology ; Drugs, Chinese Herbal ; chemistry ; isolation & purification ; pharmacology ; Flavonoids ; chemistry ; isolation & purification ; pharmacology ; Fruit ; chemistry ; Guaiacol ; analogs & derivatives ; chemistry ; isolation & purification ; pharmacology ; HT29 Cells ; Humans