Objective To investigate the anti-HBV mechanism of oxymatrine in vitro.Methods The antiviral mechanism of oxymatrine was analyzed through detecting the expression and function of TLR9 signal pathway and the secretion of antiviral cytokines from PBMCs treated by oxymatrine in vitro.Results Oxymatrine could not only induce IFN-α and IFN-γ secretions from PBMCs directly, but also effectively augmented the expressions of TLR9, MyD88 and TRAF6 at mRNA level, and activated the TLR9 signal transduction pathway. TLR9 ligand could induce IFN-α, IFN-γ and TNF-α secretion a lot from PBMCs pretreated by oxymatrine.Conclusion Oxymatrine possesses an anti-HBV activity via activation of TLR9 signal pathway and secretion of antiviral cytokines.

Objective To investigate the damage of mice liver and kidney with the compatibility application of Paeoniae radix rubra and Veratrum nigrum, and explore its mechanism. Methods We examined the alanine aminotransferase, aspartate aminotransferase (AST), lactic dehydrogenase, creatinine and urea nitrogen from serum, and glutathion peroxidase (GSH-Px) and malondialdehyde (MDA) from liver and kidney tissue after intragastric administration of different ratio (4∶1, 2∶1, 1∶1, 1∶2, 1∶4) of Paeoniae radix rubra and Veratrum nigrum in mice for 22 d. Results Paeoniae radix rubra used with Veratrum nigrum did not induce the obvious damage to liver with AST increase, but induced the obvious damage to kidney. At the same time, the depressed GSH-Px activity and the increased MDA content were observed in kidney tissue. When Paeoniae radix rubra and Veratrum nigrum is on a ratio of 2∶1, the kidney injury was the most obvious. Conclusion Paeoniae radix rubra used with Veratrum nigrum in varying proportions could induce the kidney injury in mice which is related to the oxidation-antioxidation balance disturbance.

Objective To explore the role of receptor-interaction proteins(RIP) in phenethylisothiocyanate(PEITC)-induced apoptosis in human leukemia cells.Methods U937 cells were exposed to PEITC at various concentrations of 0,2,4,6 and 8 ?mol/L for 3 and 6 h,or at 8 ?mol/L for different time intervals of 1,3,6,9,12 and 24 h.Cells were stained with Annexin V/PI,and apoptosis was determined by using flow cytometry.Total protein extracts were prepared and subjected to Western blot assay using antibodies against poly(ADP-ribose)polymerase(PARP),Caspase-3,Caspase-8,and RIP.?-actin was used to ensure equivalent loading.Results After exposure to PEITC at concentrations of 2,4,6 and 8 ?mol/L for 3 h,U937 cells showed an increase in cell apoptosis(9% ,25% ,44% and 56% respectively).While when the time expanded to 6 h,the amount of apoptotic cells was increased to 16% ,28% ,51% and 82% respectively.A dose-dependent manner was seen when U937 cells were exposure to PEITC at different concentrations.When U937 cells were treated by PEITC at concentration of 8 ?mol/L for 1,3,6,9,12,and 24 h,the apoptotic cells covered 12% ,57% ,79% ,86% ,90% ,and 91% of all cells respectively,indicating a time-dependent manner.Western blot assay showed that PEITC caused the activation/cleavage of Caspase-3 and-8,and improved the PARP cleavage,and also decreased the expression of RIP.PEITC-induced apoptosis was proceeded by degradation of RIP.Conclusion Our results indicate that PEITC induces the apoptosis in human leukemia cells through a process that involves death receptor pathway,and RIP may play an important role in the apoptosis.

Objective To study the effect of Benazepril on the renal connective tissue growth factor(CTGF) expression and interstitial fibrosis in unilateral ureteral obstruction(UUO) rats and to illuminate the possible mechanisms.Methods 24 Sprague-Dawley rats were randomly divided into Sham-operated,control and Benazepril groups.From the day before operation,the rats were under intragastric administration of Benazepril 10mg/(kg?d) in Benazepril group,and sodium chloride in tales doses in Sham and control groups.On the 14~(th) day after operation,the obstructed kidney was taken out to be measured by HE,Masson,and immunohistochemistry staining for TGF-?_1,CTGF,?-SMA and ColⅢ.Results The score of renal interstitial lesion and fibrosis index in Benazenpril were significantly lower than those in the control group(P

Objective To investigate the protective function of oxymatrine on the renal intestitial fibrosis in rats.Methods Forty male Sprague Dawley rats were randomly divided into 5 groups: sham group,model group,Lotensin group,large-dose oxymatrine group,and small-dose oxymatrine group.The models were established by unilateral ureteral obstruction(UUO) of the left side.On the 14~(th) day after operation,the obstructed kidney was taken out.Then,HE,Massion,and immunohistochemistry staining of transforming growth factor-?_(1)(TGF-?_(1)),(?-smooth) muscle acting(?-SMA) and collagen Ⅲ were conducted.After that,semiquantitative analysis was performed.Results After oxymatrine,treatment,the expressions of ?-SMA,TGF-?_(1) and ColⅢ of the obstructed kidney in the treatment group were significantly lower than those in the model group(P0.05).Conclusion Oxymatrine may reduce the expression of cytokines such as TGF-?_(1),then the activation of cell producing ECM and then the sendimentation of ECM,thus preventing renal interstitial fibrosis.

Objective To investigate the post traumatic stress disorder scores of undergraduate and the correlation between emotional approach coping(EAC) in unaffected region after Wenchuan-earthquake.Methods PTSD Check list-Civilian Version (PCL-C) and emotional approach coping scale(EACS) were used to assessed 880 undergraduates in Nanjing. Results (1)The average score of PCL-C was 30.52±9.64, and the prevalence rates of PTSD was found 5.3%.(2)Gender difference were found in social function(male:1.66±0.80,female:1.52±0.63, t =2.664,P =0.005) and positive scores of PTSD(male:31.32±10.81,female:29.81±8.41,t =2.263,P =0.022).(3)No significant difference was found in different specialty between PTSD,but in emotional procession and emotional expression.(4)Emotional-approach coping and expose time in front of media came into the regression equation of PTSD symptoms.Conclusion The score of PCL-C of undergraduate in unaffected region are infected by how long he spend in front of media and how he use emotion-approach coping strategy to cope with this shock bringing by Wenchuan-Earthquake

Objective To assess the risk of nonylphenol in reclaimed wastewater used in city to damage the human endocrine system and to present the data for making the related water quality standard. Methods Based on the monitored results of nonylphenol in the reclaimed wastewater from a reclaimed water plant in Beijing and referenced the phenol toxic parameter from integrated risk information system of the Unite State Environmental Protection Agency and the nonylphenol tested toxicity data from related literature, the risk assessment of nonylphenol on human health was conducted using four step health risk assessments when the sewage was reclaimed as a different approach to use. Results The endocrine disruption risk of nonylphenol in reclaimed wastewater on human health was about in a range of 10-8-10-10, which could even be ignored. Conclusion The risk of nonylphenol is acceptable to human health when the reclaimed wastewater were used for municipal, agricultural irrigation, urban lake supplement, then, nonylphenol as a human health risk factor can be ignored when making the standard of nonylphenol in reclaimed water.

Objective To approach the protective actions of Red Rose Capsule for Releasing the Dysmenorrhea(RRC) on the mice of primary dysmenorrhea.Method Sixty four health rats with the similar menstrual cycle were divided into four groups-NS group,RRC low dose group,RRC high dose group and Yueyueshu group.The indexes of TXB2 and 6-Keto-PGF1? in plasma were observed.Result RRC had significant effect on increasing 6-Keto-PGF1 ? and decreasing TXB2 in plasma of primary dysmenorrhea mice.Conclusion RRC has protective action against primary dysmenorrhea by influencing the content of TXB2 and 6-Keto-PGF1?.

Objective To discuss the effectiveness of heart operation through subaxillary minithoracotomy.Methods Subaxillary minithoracotomy heart operation was carried out in 378 cases between March 1995 and October 2004.The operation was performed under beating heart and without the occlusion of the ascending aorta in 81 cases of atrial septal defect, 32 cases of ventricular septal defect,and 16 cases of other diseases.The operation was completed under low temperature and extracorporeal circulation in other 249 cases.Results The operation was accomplished smoothly in all the cases.The operation time was 167?2.0 min,and the extracorporeal circulation time was 28.1?16.6 min.Postoperatively,1 patient with double-chamber anormaly of the right ventricle was given a tracheotomy because of an acute laryngeal edema,1 patient with tetralogy of Fallot died of low cardiac output syndrome,1 patient had a cerebral embolism,2 patients experienced an incision fat liquefaction,and the remaining patients recovered uneventfully.A follow-up for 2 months ~ 10 years(mean,5.1 years) in 366 patients revealed a full range of physical activities.One case of ventricular septal defect with residual shunt and 1 case of cerebral embolism were fully recovered.Conclusions Subaxillary minithoracotomy open heart operation has advantages of minimal invasion,quick recovery,simplicity of performance,and good cosmetic results.

Objective To approach the medicine-Schisandraceae-protective actions to the mice of experimental Kidney Yin deficiency from different angles. Method To observe the level of SOD and OFR in the female rats ovary tissue in order to prove the medicine’s curative effect. Result Schisandraceae can obviously increase the SOD and decrease the OFR to experimental Kidney Yin deficiency mice. Conclusion Schisandraceae has very obviously protective action to experimental Kidney Yin deficiency by influencing content of SOD and OFR of female mice ovary tissue.