Forehead ; surgery ; Humans ; Nose ; surgery ; Reconstructive Surgical Procedures ; methods ; Surgical Flaps

Objective To establish a polarographic method for detecting fluoroacetamide.Methods On the condition of strong basic solution with high temperature fluoroacetamide was decomposed to form a decomposed product ALC-La-F - complex.In a pH4.90buffer solution containing KNO 3 and hexamethylenetetramine,the decomposed product was determined with com-plex-adsorptive wave at-0.67V by polarography.Re sults The detection limit and linear range of the method was2.0?10 -8 mol/L and5.0?10 -8 -1.2?10 -6 mol/L respectively.The regressive equation was y=3.49x+0.042with a correlation coefficient r=0.9996.The method had been applied to the determination of fluoroacetamide in wheat flour samples satisfyingly.Conclu-sion This method was simple and suitable for the determination of fluoroacetamide in flour.

Objective This study was designed to analyze the proteome differences between cancer tissue and surrounding-cancer tissue using Two-Dimensional Gel Electrophoresis (2-DE) in patients with HBV relative HCC.Methods Immobile phase pH gradients (IPGs) for isoelectric focusing of proteins were used as the first dimension,and SDS-polyacrglamide gel electrophoresis(PAGE) as the second dimension. The gels were stained by silver, scanned by ImageScanner, analyzed with ImageMast software.Results Average spots expressed in cancer tissue,cirrhosis tissue and chronic hepatitis tissue were significantly different(P

Objective:To analyze and evaluate the degassing effect of vacuum degassing apparatus through the determination of ox-ygen content in pure water, gas pressure and air volume, and compare with suction filtration, boiling and ultrasonic methods to choose the appropriate degassing method for dissolution medium and guide the daily inspection work. Methods: The water residual oxygen, gas pressure and air volume were determined using a single water quality on-line monitoring instrument and a Carbo QC Anton Pear in-strument. The degassing effect of the vacuum degassing apparatus was detected under the conditions of negative pressure and certain de-gassing time. The degassing effect was also compared with that of suction filter, boiling and ultrasonic methods. Results: Under the conditions of 0. 06 MPa negative pressure, 41℃ medium temperature and 30 min degassing time, the degassing effect of the vacuum degassing apparatus was obvious. The degassing effect of suction filter and boiling methods was similar to that of the vacuum degassing apparatus, while that of ultrasonic method wasn't obvious. Conclusion:The use of vacuum degassing apparatus is simple and efficient with obvious degassing effect, which is suitable for the degassing process of dissolution medium.

Objective To investigate the effects of dexmedetomidine on hemodynamics and tra-cheal intubation facility in general anesthetized patients.Methods Forty gynecological patients of se-lective operation under general anesthesia were randomly arranged into 2 groups:group D and group C.With high flow mask oxygen inhalation(5 L/min),dexmedetomidine was intravenously pumped in-to the patients in group D at 0.6μg/kg in 10 minutes while in group C saline were given instead.Both groups were then proceeded with rapid sequence induction (RSI)immediately.The values of MAP, HR,PaO2 and PaCO2 were recorded every minute from pumping start till 15 minutes,the bucking, intubation time and glottis exposure were evaluated as well.Results No significant differences were found in intubation time or glottis exposure between these two groups.After tracheal intubation group D showed higher MAP value than group C at 14,15 minutes,and a lower HR value (P <0.05).Even no significant differences were found in values of SpO2 and PaO2 ,the group D showed less bucking than the group C (P<0.05).Conclusion Dexmedetomidine administration at 0.6μg/kg in RSI can diminish the variation in hemodynamics and facilitate the tracheal intubation by less buc-king,but no affect the oxygen reserve.

Objective:To investigate the relationship between pemphigus vulgaris(PV) and HLA-DR,DQ haplotypes in Han nations of northeast China.Methods:Polymerase chain reaction-sequence specific primers(PCR-SSP) method was used to detect the HLA-DRB1 and DQB1 alleles of 27 PV patients of Han nation of northeast China, analysed haplotyes and compared with 99 healthy controls.Results:Compared with control group, the frequencies of the haplotypes of HLA-DRB1*140x-DQB1*0503,DRB1*140x-DQB1*0201,DRB1*120x-DQB1*0503 and DRB1*140x-DQB1*0302 increased significantly in PV group. After statistical test, the difference between the two groups was significant.Conclusion:The special haplotypes may contribute to genetic susceptibility to PV in northeast Chinese.

Objective To investigate the effect of cefuroxime axetil on vaginal microecological balance in mice.Methods Twenty specific pathogen free (SPF)female mice were randomly divided into experimental group A and B and control group C and D with 5 mice in each group.Mice in experimental groups A and B were given dissolved cefuroxime axetil by gavage for 5 days,mice in the control groups C and D were given 0.9% saline instead.Vaginal secretions from experiment group A and the control group C were collected on d1 after drug withdrawal,and from experiment group B and control group D were collected on d5 after drug withdrawal.The vaginal pH,Lactobacillus,Enterobacter,Staphylococcus and Streptococcus colony forming units (CFU)were measured,and the ultrastructure of Lactobacillus was observed with scanning electron microscope (SEM).The density and diversity of vaginal flora were determined.t test and Z test were used to analyze data.Results Compared to control group C,vaginal pH was increased in experimental group A on d1 (t =2.675,P <0.05 ),and CFU of Lactobacillus,Enterobacter,Staphylococcus and Streptococcus decreased significantly (t =-2.741 , -1 3.501 , -3.077 and -2.598,all P <0.05 );compared to control group D,vaginal pH in experimental group B was increased on d5 (t =4.797,P <0.05),and CFU of Lactobacillus,Enterobacter,Staphylococcus and Streptococcus decreased significantly (t =-3.737,-6.81 6, -1 6.079 and -3.580,all P <0.05 ).The morphology of Lactobacillus showed wrinkled,broken cell wall and cell debris in experiment groups A and B under the electron microscope.The density and diversity of vaginal florain experimental groups A and B were decreased compared to those in control groups C and D (Z = -2.437 and -2.297,both P <0.05 ).Conclusion Broad-spectrum antibiotics cefuroxime axetil can increase vaginal pH, destroy the cell wall of dominant bacterial Lactobacillus,and reduce vaginal flora diversity and density,resulting in disturbance of vaginal micro-ecologicalbalance.

OBJECTIVE:To provide reference for rational use of human serum albumin for surgical inpatients. METHODS:The utilization of human serum albumin for surgical inpatients in a hospital during Jan.-Mar. 2014 was analyzed and evaluated by UHC Guidelines for the Use of Album,Nonprotein Colloid,and Crystalloid Solutions(2010 edition)and European Immune Globu-lin and Albumin Use Recommendation. RESULTS:Among the 556 patients,totally 895 human serum albumin application were con-ducted,mainly involving development of gastrointestinal surgery(29.7%),hepatobiliary surgery(25.9%)and cardiothoracic sur-gery(13.1%). The main reasons were correcting hypoalbuminemia(62.9%),followed by albumin supplemented during major sur-gery(7.9%)and alleviating ascites in patients with cirrhosis(4.4%);only 95 applications(10.6%)were considered appropriate. The most prevalent inappropriate reason was for correcting hypoalbuminemia. CONCLUSIONS:Human serum albumin in the surgi-cal inpatients in the hospital shows a large amount,and low consistent rate between indications and guidelines. The rational stan-dardized utilization of human serum albumin should be strengthened.

0.05).CONCLUSIONS There is high frequency of C.albicans in the elderly.There is no significant difference rate of detection rate among three groups.Mucosa swab sampling method may be a convenient and effective method for clinical study.

Objective: To develop an UPLC method for simultaneous determination the contents of 10 active constituents [neochlorogenic acid (NCA), chlorogenic acid (CA), cryptochlorogenic acid (CCA), isochlorogenic acid A (ICAA), isochlorogenic acid B (ICAB), isochlorogenic acid C (ICAC); bacicalin, wogonoside, baicalein, and wogonin] in Yinhuang preparations. Methods: Analysis was performed on an Acquiyt UPLC BEH C18 chromatographic column (100 mm × 2.1 mm, 1.7 μm) eluted with acetonitrile- 0.4% phosphoric acid, gradient elution, and the flow rate was 0.4 mL/min, the detection wavelength was set at 326 nm, the column temperature was 40℃, and the injection volume was 1.0 μL. Results: All calibration curves were linear (r ≥ 0.999 6) over the tested ranges. The average recoveries (n = 9) ranged from 97.43% to 99.94% with RSD value below 2.0%. The contents of 11 batches of NCA, CA, CCA, ICAA, ICAB, ICAC, bacicalin, wogonoside, baicalein, and wogonin were 0.236-3.419, 5.279-26.220, 0.495-4.714, 0.130-2.702, 0.310-3.210, 0.363-5.036, 35.209-133.289, 1.493-6.635, 1.546-5.393, and 0.254-0.823 mg/g. Conclusion: This method is rapid, simple, sensitive, accurate, and reproducible, which can be used for quality control of Yinhuang preparation, and provide a reference for the formulation of quality standards to enhance in the future.