Objective To investigate the clinical curative effect and safety of ambrocol oral liquid combined with pidotimod oral liquid in the treatment of children with mite allergic asthma.Methods 54 cases of children with mite allergic asthma from December 2014 to December 2015 were selected and randomly divided into the control group and experimental group,27 cases in each group.On the basis of routine treatment,the control group was given pidotimod oral solution 10mL,once a day,oral; on the basis of the control group,the experimental group was treated with ambrocol oral solution 10mL,twice a day,oral.Interleukin-2(IL-2),interleukin-4(IL-4),immunoglobulin E(IgE)levels,the efficacy and safety of the two groups were measured before and after treatment.Results After treatment,compared with the control group,the serum levels of IL-2 were higher,and the levels of IL-4、IgE were lower in the experimental group,the difference was statistically significant(P<0.05),the treatment efficiency of the experimental group(92.59％)was significantly higher than that of the control group(81.48％),the difference was statistically significant(P<0.05).Conclusion The ambrocol oral liquid combined with pidotimod oral liquid can significantly improve the serum level of IL-2,and reduce the serum level of IL-4,IgE in children with mite allergic asthma,improve clinical efficacy.

Objective To study the effect of urapidil on the levels of 4 kinds of plasma neuropeptides : endothelin(ET),neuropetid Y(NPY),calcitonin gene-related peptide(CGRP) and neurotensin(NT) in patients with hypertension. Methods Twenty patients with hypertension ,scheduled for surgery,received urapidil 0.5mg/kg intravenously before anesthesia induction .The intravenous blood samples were taken before and 5min after the administration ,to measure the plasma levels of ET,NPY,CGRP and NT with radioimmunoassay . Twenty health humans served as control. Results Compared with control levels, ET and NPY levels increased markedly and CGRP and NT levels decreased significantly in the patients with hypertension (P

Adult ; Coal Mining ; Cognition Disorders ; etiology ; Explosions ; Follow-Up Studies ; Humans ; Surveys and Questionnaires ; Survivors ; Young Adult

China ; Food Safety ; Risk Assessment

Objective To investigate the relationship between obesity parameters and obstructive sleep apnea-hypopnea syndrome (OSAHS) in aircrew. Methods A questionnaire survey in aircrew members was performed. Outcome measurement included body height, body weight, neck circumference ( NC), waist circumference (WC), and body mass index (BMI). Those with snoring during sleep and/or Epworth sleepiness scale (ESS)score ≥ 9 were screened with pulse oxygen saturation test during overnight sleep. Those with oxygen saturation decrease index ≥ 10 times/h and suspected OSAHS were tested by polysomnography (PSG). The objects were then assigned to three groups: the OSAHS group,the snore group and the normal group. The relationship between obesity parameters and OSAHS was analyzed. Results There were 399 ( 37. 54% ) overweight, 36 ( 3. 39% ) obesity, 130 ( 12. 23% ) increased NC, and 354 (33.30%)increased WC in 1063 aircrew members (OSAHS group ＞ snore group ＞ normal group; P＜O. 05). The mean value of BMI, NC and WC in the three groups were dittos. Multiple logistic regression analysis showed that overweight, obesity and increased WC were significantly associated with snoring (P ＜0.05) and snoring enlarged NC was significantly correlated with OSAHS (P＜ 0.05). Conclusion Increased BMI, NC and WC may be risk factors of OSAHS among aircrew. Effective prevention and treatment of OSAHS should be needed.

Objectives To explore the effects of hemodialysis on oxidizing stress and plasma nitrogen monoxide (NO) levels in maintenance hemodialysis patients. Methods 67 maintenance hemodialysis patients (male 39, female 28, and mean age 58?13y) were enrolled in this study. Serum malondialdehyde (MDA) level was measured by colorimetry, and plasma NO level was determined by enzymatic method. The MDA and NO levels before and after hemodialysis were measured in 48 cases of the patients. Results After hemodialysis, the serum MDA levels significantly increased, and the plasma NO levels decreased. The patients with cardiovascular diseases had lower NO levels and higher MDA levels than the patients without ones before hemodialysis. The serum MDA levels of the patients using biological incompatible dialysis membrane increased after hemodialysis. Conclusion Hemodialysis treatment, especially using biological incompatible dialysis membrane increased oxidizing stress and decreased plasma NO levels in maintenance hemodialysis patients, and was significantly correlated with the pathogenesis of cardiovascular diseases.

OBJECTIVE:To observe the effects of bisoprolol combined with conventional triple therapy on the efficacy and re-lated indexes of patients with rheumatic heart valvular disease (RVD) combined chronic heart failure. METHODS:110 patients with RVD combined with chronic heart failure were randomly divided into observation group(55 cases)and control group(55 cas-es). All patients were treated with oxygen inhalation,anti-infection,correcting electrolyte imbalance,controlling arrhythmia,and giving digitalis drugs and diuretic drugs(it can be disabled after edema symptom being controlled);based on it,control group was given Enalapril tablet with initial dose of 2.5 mg,once to twice a day,then adjusted to 10-20 mg after 1 week,twice a day+20 mg Spironolactone tablet,once a day+10 mg Propranolol tablet,once a day. Observation group was additionally given Bisoprolol fuma-rate tablet initial dose of 1.25 mg,once a day,and then increased to 10 mg based on condition improved and tolerability after a meal,once a day. They were treated for 12 weeks. Clinical efficacy,and LVEF,LVEDD,LVESD,SBP,DBP,HR before and af-ter treatment and incidence of adverse reactions in 2 groups were observed. RESULTS:The total effective rate in observation group was significantly higher than control group,the difference was statistically significant (P<0.05). Before treatment,there were no significant differences in the LVEF,LVEDD,LVESD,SBP,DBP and HR between 2 groups(P>0.05). After treatment,LVEF in 2 groups were significantly higher than before,and observation group was higher than control group,LVEDD,LVESD,SBP, DBP and HR were significantly lower than before,and observation group was lower than control group,the differences were statis-tically significant(P<0.05). And there was no significant difference in the incidence of adverse reactions(P>0.05). CONCLU-SIONS:Based on conventional treatment,bisoprolol combined with conventional triple therapy shows good efficacy in the treat-ment of RVD combined chronic heart failure,it can improve cardiac function,with good safety.

Objective To further identify the isolated Mycoplasma genitalium (Mg) from the high risk population of STD in China. Mothods The morphology of Mg standard strains and clinical isolates were observed under electron microscopy (EM) and atomic force microscopy (AFM). Mg sample preparation for AFM was fixed on mica and AFM images of scanning observation were captured by contacting and tapping modes and operated under normal atmospheric pressure and temperature. Results and Conclusion Mg had a diversity of shapes, including flask, pear, spindle or round shape with projecting neck portion and slightly broadened terminal structure under EM. The morphologic features under AFM were similar to those observed by EM. The typical Mg showed shapes of flask and pear with noticeable narrow ring or mark in the neck portion. The size of Mg measured under EM and AFM was similar as well. Mycoplasma

Preparations of cell-free supernatant from Rous sarcoma "?????" were inocu-lated onto the chorio-allantoic membranes (CAM) of the chick embryos of 7--9 days in-cubation. The inoculated portions of the CAM were removed at various intervals from6 hours to 10 days afterwards. The tissues were fixed in Carnoy's solution and werestained with methyl green-pyronin for nucleic acid, Alcian blue-hematoxylin or Alcianblue-alum cochineal for acid polysaccharide and toluidine blue for metachromatic reac-tion. The pathogenesis of the Rous sarcoma of the inoculated CAM and the histo-chemical reaction of the nucleic acid and acid polysaccharide have been followed by his-tological examinations. Recognizable macroscopic lesions consisting of membrane opacity, focal thickening,oedema and discrete nodules in the inoculated CAM appeared from the first day on-ward. Distinctive hemorrhagic lesions and pocks were observed on the 3rd to the 5thday. Tumor masses of different sizes appeared from the 7th to the 9th day. In casethe CAM were inoculated with horse serum-saline, no or only slight reaction was ob-served, and the normal thickness and structures of the CAM were generally maintained. Three successive stages of the Rous sarcoma on the inoculated CAM weredistinguished: (1) Focal ecto-mesodermal proliferation with formation of earlytumor-like nodules; (2) Extreme ectodermal hyperplasia and early mesodermalsarcoma-like proliferation; (3) Ectodermal necrosis and extensive malignant growthof the mesodermal neoplastic cells. The fibroblasts, pyroninophilic round cells andthe ectodermal cells were the main types of cells concerned in the developmentof tumors following infection of the CAM Rous virus. The fibroblasts transformedinto tumor cells and became the most conspicuous elements of the Rous sarcoma. Thepyroninophilic round cells were considered to be belonging to the basophilic myelocyteseries, according to their morphological and staining characteristics. Their possible rolein the tumor infiltration was discussed. The intensity of the nucleic acid in the CAM cells increased along with the increaseof histopathological changes at the inoculated area of the membrane. The possible sig-nificance of their parallel relationship was discussed. Alcian blue staining of the growing tumor tissue demonstrated the presence of acidpolysaccharide reaction in the ground substance. The intensity increased with the increaseof the malignancy of the neoplastic cells. This material is non-metachromatic whenstained with toluidine blue and could be removed by treatment with testicular hyaluroni-dase for 3--5 hours. The mucin present is considered to be of a hyaluronic acid nature.

Objective To research the surgical technique and curative effect of the posterior cruciate ligament(PCL) anatomical reconstruction with double-bundle hamstring tendons absorbable interference screw fixation via double-tunnel in femoral under arthroscopy. Methods 24 patients, average age of 32 years ranged from 17 to 42 years old, were treated with anatomical reconstruction of PCL through double-tunnel in femoral and one tunnel in tibia with double-bundle hamstring tendons fixed with absorbable interference screw under arthroscopic. The anterolateral bundle was reconstructed with multistranded autograft semitendinosus tendon grafts and was tightend and fixed, at knee flexion 15?-30?. The posteromedial bundle was reconstructed with multistranded autograft gracilis tendon grafts, and was tightened and fixed, at knee flexion 60?-70?. Results All the patients were followed up for a mean time of 9.4 months (6 months-19 months). All the knee joint motion were in normal range, no exhibition of pain and distinct trauma arthritis. The postoperative posterior drawer test, reverse Lachman test, tibia sags sign and reverse pivot shift test were all negative. The postoperative Lysholm score was (92.7?6.4) points, and the postoperative score increased significantly compared with the preoperative (t=3.12, P