Objective To understand the internal structure,loss direction of loss nursing staff, compare the current condition of nurses loss between minorities and Han nationality nurses, analyze the relationship between nurse turnover intention and job satisfaction in second-class and upwards general hospital of Xinjiang. Methods Adopting stratified random method, nursing staff from 61 second-class and upwards general hospitals were selected to participate the questionnaire survey. Results Loss of nurses in Xinjiang increased year by year. Nurses with age under 35 years old, college education, employment and with primary titles were most vulnerable to loss. The turnover rate of nurses with Han nationality was higher than minority nurses. Income, occupational status, learning assistance services, opportunities for personal advancement and retirement benefits constituted negative correlation with turnover intention, among which income level was most related. Health care services, work scheduling arrangements and the components of job independence demonstrated no significant correlation. Conclusions To improve income level of nurses and increase working enthusiasm of nurses with higher education and employment nurses, especially nurses with Han nationality is an important means to reduce the loss of nurses.

Objective To investigate the change of the serum matrix metalloproteinase-13 (MMP-13) and tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) and the correlations of MMP-13 and TIMP-1 with bone metabolic markers or bone mineral density(BMD) in aged postmenopausal Chinese women.Methods One hundred and twenty women aged 48-65 years with postmenopausal were selected as subjects.They were divided into normal group(n =28),low bone mass group (n =36) and osteoporosis group (n =56) accordance with the WHO standard.The levels of serum MMP-13,TIMP-1,estradiol (E2),Osteoprotegerin (OPG),Osteoprotegerin ligand (OPGL),Procollagen terminal propeptide of N (PINP),and Type Ⅰ collagen cross-linked C terminal peptide(CTX) were measured using enzyme linked immunosorbent assay(ELISA).And the ratios of MMP-13 to TIMP-1 were calculated.Bone mineral density(BMD) of the lumbar spine,femoral neck,Fahrenheit and greater trochanter were measured using dual energy X-ray absorptiometry.Results There was significant difference of serum MMP-13 concentrations in normal controls,low bone mass group and osteoporosis group((27.08 ± 1.41) μg/L,(45.64 ± 1.62) μg/L and (44.25 ± 1.21) μg/L; F =110.314,P =000),and serum MMP-13 concentrations in low bone mass group and osteoporosis group higher than that in normal controls (P ＜ 0.05),serum MMP-13 concentrations in low bone mass group higher than that in osteoporosis group,but there was on significant difference(P ＞ 0.05).But Serum TIMP-1 concentrations in three groups was no significant difference (F =10.721,P =0.801).The ratio between MMP-13 and IIMP-1 was 0.185 ± 0.062,0.311 ± 0.053,0.332 ± 0.063 respectively,and there was significant difference of the three groups (F =137.771,P =0.000),and the ratio of the low bone mass group and osteoporosis group higher than the normal group(P ＜0.05),but there was no significant difference between the two groups.In osteoporosis group,Negative correlations between MMP-13,and BMD,E2 and OPGL,were found (r =-0.296,-0.198,-0.301,-0.298,-0.233 respectively,P ＜ 0.05).Meanwhile positive relations were found between MMP-13 and OPG,PINP,CTX (r =0.228,0.315,0.312 respectively,P ＜ 0.05).In low bone mass group,the level of MMP-13 was significantly relative with BMD (the lumbar spine,Fahrenheit),E2 and CTX (r =-0.188,-0.196,-0.235,0.289 respectively,P ＜ 0.05).Conclusion There are significant correlations between serum MMP-13,ratios of MMP-13 and TIMP-1 and bone metabolism marker and BMD.MMP-13 level may increase with increases in osteoporosis and osteopenia.The increases of serum MMP-13 and the decrease of ratio of MMP-13 to TIMP-1 might be biomarkers in high bone turnover state,such as postmenopausal osteoporosis and early stage of osteopenia.

Objective To observe the influence of ulinastatin for the gastrointestinal function and inflammatory stress state of patients with severe acute pancreatitis.Methods 46 patients with severe acute pancreatitis in our hospital from October 2015 to July 2016 were randomly divided into control group and observation group with 23 cases in each group, the control group was treated with routine treatment of severe acute pancreatitis , the observation group was treated with ulinastatin on the basic treatment of control group, then the serum gastrointestinal hormones before and after meal and inflammatory stress indexes of two groups at different time before and after the treatment were compared .Results The serum gastrointestinal hormones before and after meal and inflammatory stress indexes of two groups before the treatment were compared,the differences were not significant,while the serum gastrointestinal hormones and inflammatory stress indexes of observation group after the treatment were all better than those of control group,the differences were all significant(all P<0.05).Conclusion The ulinastatin can significantly improve the gastrointestinal function and inflammatory stress state of patients with severe acute pancreatitis, so the application value in the patients with severe acute pancreatitis is higher.

In this paper, 29 endophytes were isolated from different organs and tissues of Lycium barbarum of Ningxia by tablet coating method, 18 of them was fungi, and 11 of them was actinomycetes. The endophytes quantity in the different tissues were leaves > flowers > roots >fruits; The hydroxyl radical scavenging activities of 11 endophytes were investigated by Fenton reaction, and total antioxidant capacities of them were examined by a. total antioxidant capacity test kit; culture features and strain-specific sequence analysis were employed to explore the diversity of the 11 endophytes. The result showed that 5 fungi and 6 actinomycetes that having antioxidant activity could be phylogenetically classified into 3 genera, 3 genera and 3 families, respectively. The total antioxidant capacity and hydroxyl radical scavenging activity of the 11 endophytes showed distinct difference. The antioxidant activity of Aspergillus were stronger, among which total antioxidant capacity of fL1 was (188.5 ± 0.549) U · mL⁻¹ and the IC₅₀ was 0.3 mg · L⁻¹; the IC₅₀ of strain fL1 was 0.42 mg · L⁻¹ and the total antioxidant capacity of fL9 was (113.63 ± 1.021) U · mL⁻¹, all of them were stronger than the positive control Vit C. The experimental results indicated that endophytic fungi of L. barbarum of Ningxia have a great developing and application prospect for the development of antioxidant agent.
Antioxidants ; chemistry ; Bacteria ; chemistry ; classification ; genetics ; isolation & purification ; Biodiversity ; Endophytes ; classification ; genetics ; isolation & purification ; Fungi ; chemistry ; classification ; genetics ; isolation & purification ; Lycium ; microbiology ; Molecular Sequence Data ; Oxidation-Reduction

Study of the strategic objectives chosen by hospitals from the perspective of stakeholders is now under the spotlight of researchers at large. To identify whether there exist differences in strategic objectives among various groups in the hospital and probe into the causes, the author called into play the competing values framework as the analysis tool. An evidenced-based study on public hospitals in Hangzhou identified marked differences on choices of strategic objectives among various internal stakeholders in the hospital For example, hospital administrators tend to focus on internal control, while medical doctors tend to focus on interactions between the hospital and the external environment. On this basis, further studies are proposed.

An HPLC-UV method has been developed for the determination of valibose, miglitol, voglibose and acarbose, the four anti-diabetic drugs. The separation was accomplished successfully by using reversed phase chromatography (Prevail carbohydrate column, 250 mm x 4.6 mm, 5 microm) with a gradient acetonitrile-phosphate buffer solution (pH 8.0) at a wavelength of 210 nm. Furthermore, the method of a high-performance liquid chromatography coupled with ESI-MS in positive ionization mode has been established. These two methods were successfully applied to the assay and qualitative detection of four alpha-glucosidase inhibitors in the potential counterfeit anti-diabetic drugs.

Objective To evaluate the capability of strain rate imaging(SRI)for monitoring regional systolic deformation and synchronicity of left ventricle after coronary artery bypass graft(CABG)and for evaluating effect of surgery and predicting restenosis.Methods The values of systolic strain rate(SRsys),systolic strain(Ssys)and post systolic strain index(PSI)in 5 segments supplied by left anterior descending coronary artery were measured in study group(60 patients with coronary artery disease)at 1 day before and 10 days,1 month,3 months and 6 months after CABG.Forty healthy participants served as a baseline control group.The regional myocardial function before and after CABG was compared and analyzed.Results The peak values of SRsys and Ssys decreased before CABG in study group.In 52 of the 60 patients,SRsys and Ssys in the graft segments increased gradually and showed statistical significance in most studied segments at 3 and 6 months after surgery.In the above 52 patients,value of PSI increased before CABG and reduced significantly in all analyzed segments at 6 months after surgery.The restenosis of graft artery was suspected in 8 patients by SRI and the positive predictive value was 75%.The diagnosis sensitivity of SRI parameter method was higher than that of 2-dimensional echocardiography and the sensitivity of Ssys was higher than that of SRsys.Conclusions SRI can be used to quantitatively assess the regionsl systolic deformation and synchronicity and monitor the improvement of myocardial function after CABG and determine the effect of surgery and predict restenosis of graft artery.

Objective To evaluate the role of Akt and nuclear factor (NF)-κB pathway in the development of chemoresistance in gastric cancer and the relation between Akt and NF-κB.Methods SGC-7901 cells were exposed to chemotherapeutic drugs (doxorubicin and etoposide ) or chemotherapeutic drugs combined with Wortmannin or MG-132.The cell growth was detected using MTT method.The apoptosis of SGC-7901 cells was measured by TUNEL and Annexin V/PI methods.The protein level of NF-κB was analyzed by immunocytochemical staining.Electrophoretic mobility shift assay (EMSA) was used to confirm the increased nuclear translocation of NF-κB/P65.chemotherapeutic drugs could obviously inhibit the growth of SGC-7901 cells in time-dose-dependent manner.Pretreatment of SGC-7901 cells with Wortmannin or MG-132 could promote this inhibitory κB in a dose-dependent manner.Wortmannin or MG-132 pretreatment could enhance the apoptosis of NF-κB was found in SGC-7901 cells stimulated with Wortmannin,but no activation of Akt was noted in those treated with MG-132.Conclusions The chemotherapeutic drugs can both induce apoptosis and activate Akt and NF-κB in SGC-7901 cells.The efficacy of chemotherapeutic drugs can be increased via inhibiting activation of Akt or NF-κB.

Objective:To study the boiling water brewing extraction of astragalus polysaccharides in order to solve the problems of long cycle , high energy consumption and poor clarity in the traditional extraction , and lay foundation for the industrial production . Methods:Using the extraction yield of astragalus polysaccharide as the evaluation index and phenol -sulfuric acid method applied to de-termine the content of astragalus polysaccharide , the boiling water brewing extraction was used for astragalus polysaccharides .The effects of brewing time , boiling water amount and brewing times on the polysaccharide content were studied by single factor experi -ments, and then an orthogonal design method was used to screen the optimum technology parameters .The traditional water decoction extraction process was employed as the control .Results:The optimum conditions of boiling water brewing were as follows:adding 9-fold amount of water and soaking 60 min for three times.The astragalus polysaccharides extraction yield (4.81) of the optimal technology was higher than that of the traditional water decoction extraction (4.06%).Conclusion:Boiling water brewing method used to extract astragalus is with high extraction yield , simple operation , short cycle and low energy consumption , the color of astragalus polysaccha-ride is light, and it is clear after dissovled in water , which is superior to the traditional water decoction extraction method , and provide a new method with broad application prospect for the preparation of astragalus related preparations .

BACKGROUND: A stable and exact animal model is the necessary tool and basis for studying hemorrhagic cerebrovascular diseases.OBJECTIVE: To establish and evaluate the intracerebral capsular hemorrhage models in rats.DESIGN: A randomized and controlled animal experiment.SETTING: Second Hospital Affiliated to Xuzhou Medical College; First Hospital Affiliated to Nanjing Medical University.MATERIALS: This experiment was carried out in the animal experimental center of Nanjing Medical University during May to November 2002.Totally 35 SD rats were randomized into two groups: experimental group (n=30) and sham-operation group (n=5).METHODS: ① Autoblood was injected into the intracerebral capsule of rats to create intracerebral capsule hemorrhage models with stereotaxy in the experiment group. ②Scoring was conducted according to 5-point neurological scoring criteria from ZeaLonga, somatic sensation and motor function of rats were observed. ③Somatosensory evoked potential(SEP) of rats was detected pre- and post-operation under anesthetic state. ④ After determination of SEP, the rats were sacrificed under anesthetic state. Brains were taken out to made slices, then sections were stained with haematoxylin and eosin. Changes in haematoma and histomorphology were observed at the largest focus under optical microscope.MAIN OUTCOME MEASURES: ①Nerve function scoring; ②Latent period of various waves of SEP; ③ Observation of brain tissue morphology.RESULTS: Totally 35 rats entered the stage of result analysis. ①Appearance of obvious paralysis of the rats suggested the modeling was successful. The successful rate of this experiment was 93.3%(28/30). Significant difference existed in neurological scoring between experimental group [(2.74±0.46)points] and sham-operation group (0 point)(P＜0.05). ②SEP showed that the latent periods of various waves of experimental group after operation were significantly delayed than those before operation and those of sham-operation group [P1: (15.72±0.78) ms, (10.69±0.52) ms, (10.73±0.48) ms;Nl: (17.95±1.27) ms,(13.21±1.31) ms, (13.34±1.27) ms;N2:(21.16±1.62) ms, (15.42±1.46) ms,(15.58±1.44) ms;N3:(24.86±1.58) ms, (18.72±1.76) ms, (18.99±1.67) ms,P＜0.05]. ③In the shamoperation group, a few red blood cells were scattered in the peripheral area of needle channel were found, but hemorrhagic focus was not; In the experimental group, irregular or oval blood clots presented in the left internal capsule area. In about a low-fold visual field, brain tissue in the surrounding of hemorrhagic focus was loosened and swelled, and pathological changes were obviously severer than those in the sham-operation group.CONCLUSION: Intracerebral capsular hemorrhage induced by injection of autoblood with stereotaxy is more close to clinical situation, and it is easy to operate and has good reproducibility.