1.THE SIGNIFICANCE OF CHANGES IN TISSUE SUPPRESSORS OF CYTOKINE SIGNALING IN RATS WITH SEPSIS INDUCED BY CECAL LIGATION AND PUNCTURE
Hongyun LI ; Yongming YAO ; Nin DONG
Medical Journal of Chinese People's Liberation Army 2001;0(09):-
The aim of this study was to observe the changes in tissue suppressors of cytokine signaling (SOCSs) mRNA expression, and to investigate their potential role in the pathogenesis of sepsis induced by cecal ligation and puncture (CLP). Fifty four Wistar rats were randomly divided into three groups: normal control group ( n =6), CLP induced sepsis group ( n =36) and recombinant bactericidal/ permeability increasing protein (rBPI 21 ) treatment group ( n =12). Tissue samples from the liver, lung and kidney were collected to determine SOCS1 and SOCS3 mRNA expressions. Meanwhile, tissue endotoxin and TNF ? levels were also determined. The results showed that, after CLP, endotoxin and TNF ? levels in the liver, lung and kidney significantly increased, peaking at 2~12h ( P
2.SIGNIFICANCE OF CHANGES IN HIGH MOBILITY GROUP-1 PROTEIN IN RATS WITH POSTBURN STAPHYLOCOCCUS AUREUS SEPSIS AND ITS POTENTIAL SIGNAL TRANSDUCTION PATHWAY
Hongyun LI ; Yongming YAO ; Nin DONG
Medical Journal of Chinese People's Liberation Army 2001;0(09):-
The aim of this study was to investigate changes in high mobility group 1 (HMG 1) protein in postburn Staphylococcus aureus infection and its potential signaling mechanism. A total of 61 male Wistar rats were randomly divided into four groups as follows: normal control group ( n =6), scald control group ( n =9), postburn sepsis group ( n =36) and rapamycin (RPM) treatment group ( n =10). Tissue samples from the liver and kidney were collected to determine tumor necrosis factor ? (TNF ?) and HMG 1 mRNA expression. The results showed that, after Staphylococcus aureus challenge, TNF ? mRNA expression was up regulated rapidly in both the liver and the kidney, peaking at 2 hours ( P
3.Ginkgo biloba extract protection in acute paraquat poisoning of rat lung tissue .
Jian-nin SU ; Xin-hai LI ; Hui DONG ; Hui CHEN ; Xian-li GUO ; Yin-ping TIAN ; Hen-wen SHI ; Shu-hua HUO
Chinese Journal of Industrial Hygiene and Occupational Diseases 2003;21(3):226-227
Acute Disease
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Animals
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Ginkgo biloba
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Glutathione
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analysis
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Lung
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drug effects
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metabolism
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pathology
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Malondialdehyde
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analysis
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Paraquat
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toxicity
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Phytotherapy
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Plant Extracts
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therapeutic use
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Poisoning
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drug therapy
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Rats
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Superoxide Dismutase
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analysis
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Treatment Outcome
4.The changes of funny currents in the ventricular myocytes of neonatal rats and adult rats.
Hong-xia LI ; Xiang-jun YANG ; Xin ZHAO ; Ru-xin WANG ; Nin-zheng DONG ; Lian-hua HAN ; Ya-feng ZHOU ; Bin JIANG ; Wen-ping JIANG
Chinese Journal of Applied Physiology 2008;24(4):411-415
AIMTo record funny currents (If) of ventricular myocytes and to analysize hyperpolarization-activated cation channel(HCN) expression in the rats of different ages.
METHODSFresh ventricular myocytes were isolated from 3 days rats and adult rats.HCN expressions were measured by real-time quantitative polymerase chain reaction(real-time PCR). It was recorded through whole-cell patch clamp.
RESULTSHCN1, HCN2, HCN3, HCN4 mRNA represented 0.23% +/- 0.01%, 83.58% +/- 0.04%, 0.79% +/- 0.01%, 15.44% +/- 0.01% of total HCN mRNA in the neonatal rats, respectively. If was recorded and the threshold for activation was -75 mV. In the adult rat, HCN1, HCN2, HCN3, HCN4 mRNA represented 0.72% +/- 0.02%, 91.58% +/- 0.08%, 0.27% +/- 0.02%, 7.12% +/- 0.02% of total HCN mRNA. The ratio of HCN2 to HCN4 was approximately (13.06 +/- 0.21):1. The threshold for activation of If was approximately -115 mV in the adult rats.
CONCLUSIONWith the development of rats, the value of If is smaller. The threshold for activation of If is more negative. The ratio of HCN2 to HCN4 is bigger.
Age Factors ; Animals ; Animals, Newborn ; Cells, Cultured ; Cyclic Nucleotide-Gated Cation Channels ; metabolism ; physiology ; Heart Ventricles ; cytology ; Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels ; Ion Channels ; metabolism ; Myocytes, Cardiac ; cytology ; physiology ; Patch-Clamp Techniques ; Potassium Channels ; metabolism ; physiology ; RNA, Messenger ; metabolism ; Rats ; Rats, Sprague-Dawley
5.Asian society of gynecologic oncology workshop 2010.
Dong Hoon SUH ; Jae Weon KIM ; Mohamad Farid AZIZ ; Uma K DEVI ; Hextan Y S NGAN ; Joo Hyun NAM ; Seung Cheol KIM ; Tomoyasu KATO ; Hee Sug RYU ; Shingo FUJII ; Yoon Soon LEE ; Jong Hyeok KIM ; Tae Joong KIM ; Young Tae KIM ; Kung Liahng WANG ; Taek Sang LEE ; Kimio USHIJIMA ; Sang Goo SHIN ; Yin Nin CHIA ; Sarikapan WILAILAK ; Sang Yoon PARK ; Hidetaka KATABUCHI ; Toshiharu KAMURA ; Soon Beom KANG
Journal of Gynecologic Oncology 2010;21(3):137-150
This workshop was held on July 31-August 1, 2010 and was organized to promote the academic environment and to enhance the communication among Asian countries prior to the 2nd biennial meeting of Australian Society of Gynaecologic Oncologists (ASGO), which will be held on November 3-5, 2011. We summarized the whole contents presented at the workshop. Regarding cervical cancer screening in Asia, particularly in low resource settings, and an update on human papillomavirus (HPV) vaccination was described for prevention and radical surgery overview, fertility sparing and less radical surgery, nerve sparing radical surgery and primary chemoradiotherapy in locally advanced cervical cancer, were discussed for management. As to surgical techniques, nerve sparing radical hysterectomy, optimal staging in early ovarian cancer, laparoscopic radical hysterectomy, one-port surgery and robotic surgery were introduced. After three topics of endometrial cancer, laparoscopic surgery versus open surgery, role of lymphadenectomy and fertility sparing treatment, there was a special additional time for clinical trials in Asia. Finally, chemotherapy including neo-adjuvant chemotherapy, optimal surgical management, and the basis of targeted therapy in ovarian cancer were presented.
Asia
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Asian Continental Ancestry Group
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Chemoradiotherapy
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Endometrial Neoplasms
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Female
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Fertility
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Humans
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Hysterectomy
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Laparoscopy
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Lymph Node Excision
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Mass Screening
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Ovarian Neoplasms
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Uterine Cervical Neoplasms
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Vaccination