Online learning is now becoming a teaching norm across disciplines and educational contexts. There is a need to measure the satisfaction level objectively and comprehensively to allow refinement of teaching and learning delivery methods. To date, there is no English validated scale to measure learners’ satisfaction when learning is conducted online. We aimed to translate and validate the E-Course Satisfaction Scale which is originally in Turkish into English among undergraduate medical students. We found that this 35-items with five-point Likert-type responses English version of the E-Course Satisfaction Scale is valid and reliable to measure students’ satisfaction on their e-learning experiences.