Objective To explore the prognostic value of the content of lentil lectin-reactive alphafetoprotein-L3(AFP-L3)in early-stage hepatocellular carcinoma(HCC).Methods According to the content of alpha.fetoprotein(AFP)and AFP-L3 before the treatment,97 patients with early-stage HCC were divided into group A(AFP＞20 μg/L & AFP-L3＜15%,n=29),group B(15%≤AFP-13≤50% & 20 μg/L≤AFP≤200 μg/L,n=16),group C(AFP-13＞50%& AFP＞200 μg/L,n=13),group D(AFP-L3＞50% & 20 μg/L≤AFP≤200 μg/L,n=24)and group E(15%≤AFP-L3≤50%& AFP＞200 μg/L,n=15).The degree of tumor differentiation,the 1-,2-,3-year survival rates and tumor-free survival rates of the patients were analyzed.Results The degree of tumor differentiation.3-year survival rate and tumor-free survival rate of patients in group A were significantly higher than those in the other 4 groups(χ2=21.051,10.043,4.450,6.977,25.566,P＜0.05).The degree of tumor differentiation,1-,2-,3-year survival rates and tumor-free survival rates of the patients in group C and D were significantly lower than those in other 3 groups(χ2=7.938,3.488,9.085.P＜0.05).Conclusions The increase of AFP-L3 content is closely related to poorly differentiated HCC and bad prognosis.especially when the AFP Ievel is low.The detection of AFP-L3 content before and after operation is beneficial to the evaluation of the prognosis of HCC patients.

Objective To explore the short and long term result and the impact on cell immunity of splenic radiofrequence ablation in patients with hypersplenism.Methods In this study 43 patients were divided into the RFA treatment group(22 cases)and open splenectomy control group(21 cases).Blood samples were collected for blood routine,lymphocyte transformation efficiency,NK cell activity and T lymphocyte subpopulations at 1 week,1 month,6 month,1 year,2 years and 3 years before and after operation respectively and the results were compared between the two groups.Results The hypersplenism of the patients in both groups were relieved.There were no significant difierences in cell indices between the postoperative and preoperative values in the two groups.In the control group,compared with the preoperative values in the trial group,the postoperative NK cell activity and lymphocyte transformation efficiency were significantly reduced(P＜0.05).Even though,with time,the NK cell activity and lymphocyte transformation efficiency were on an increasing tendency after operation but still significantly lower than that before operation.In the control group,compared with the preoperative values,CD4+T cell count and CD4+/CD8+ratio decreased significantly(P＜0.05).Moreover.with time.these indices were on an increasing tendency.CIM+T cell count and CD4+/CD8+ ratio restored to the preoperative values within 30 months(P=0.078)and 36 months(P=0.103)respectively.Conclusion RFA treatment for hypersplenism preserves a part of the spleen and thus preserves patient's cell immunity.This treatment exhibits microinvasiveness and few complications and being easy to operate.Hence,RFA treatment for hypersplenism merits wide clinical application.

Objective:This study was conducted to further investigate the clinical value of ERCP plus EST and pancreatic duct (PD) stent placement in treatment of ABP.Methods:The data of 115 patients with ABP in People′s Hospital of Chongqing Banan District between February 2018 to October 2020 were retrospectively analyzed. Of the patients, 46 cases received ERCP plus EST and PD stent placement(PD stent group), and other 69 cases only received ERCP plus EST(control group), all patients received surgery within 72 h. Outcome measures: preoperative complications, APACHE II score, Glasgow score, preoperative and postoperative laboratory indicators (white blood cell, C-reactive protein, serum amylase, total bilirubin, alanine aminotransferase), postoperative complications, hospitalization time, hospitalization cost. All patients received outpatient or telephone follow-up after discharge, patients were followed up for recurrence of pancreatitis and complications, follow-up ended in June 2021. Continuous data were represented as ( ± s), and comparisons between the two groups were performed using Student′s t tests for normally distributed data with homogeneity of variance. The Mann-Whitney U test was used for nonnormally distributed data. Categorical data are expressed as rates values and were analyzed with the chi-square test or Fisher′s exact test. Results:There were no significant differences between two groups in age, complications, APACHEII score, Glasgow score, preoperative laboratory examination, postoperative CRP, total bilirubin and ALT ( P>0.05). Postoperative WBC[8.5(7.6, 10.3)]×10 9/L, serum amylase [197.5(143.0, 256.0) U/L] in the PD stent group were significantly lower than control group[9.9(8.2, 12.8) 10 9/L, 270.0(168.0, 419.0) U/L]( P<0.05). The overall incidence of complications in the PD stent group (6.5%) was significantly lower than the control group (20.3%), there were statistical differences between groups( P<0.05). Hospitalization time and hospitalization cost were not significantly different between the two groups ( P> 0.05). All patients were followed up, with an average follow-up of 16 months, all recovered well postoperatively in patients with stent group, but a patient who suffered from peripancreatic abscess with severe infection needed hospitalization again in control group, another two patients with pancreatic pseudocyst, 1 case were followed up for 3 months to gradually absorbed, and 1 case underwent another surgical treatment. Conclusion:The placement of temporary pancreatic duct stent provided adequate drainage of pancreatic fluid to reverse the course of ABP, and the complication rate was significantly lower than that of the control group, with superior clinical outcome to ABP patients treated with ERCP+ EST alone.