Objective To explore the long-term effects of bubble baths on seizure-induced neurobehavior deficits and the expression of apoptotic/autophagic marker B cell lymphoma/leukemia-2 ( Bcl-2 ),Beclin-1,and plasticity-related gene-1 ( PRG-1 ) in newborn rats. MethodsSixty rats aged 6 days were randomly divided into 4 groups:a control group (CON),a control hydrotherapy group (HCON),a recurrent-seizure group (RS) and a recurrent-seizure hydrotherapy group (HRS),with 15 in each group.Flurothyl was used to induce 30 min of seizures daily for 6 consecutive days in the RS and HRS groups.Rats in the CON and HCON groups were placed in the same container for equal duration without exposure to flurothyl.Rats in the HCON and HRS groups were given bubble baths for 28 consecutive days after the end of the last seizure.Neurobehavioral damage was observed using open field behavior at postnatal day 26 (P26) and Morris water maze performance at postnatal day 43 to 49 (P43-P49) and a single-blind method.PRG-1,Bcl-2 and Beclin-1 protein levels in the hippocampus were detected by Western blotting at postnatal day 50 (P50). Results①The average open field test scores of the RS rats decreased significantly compared with those of the CON and HRS rats at P26.②In the Morris water maze test the average latencies of all rats decreased gradually from the 1st to 5th days (d1 to d5) after establishment of seizure model.The average escape latency was significantly longer for rats of the RS group than for CON group rats at the 4th and 5th days ( d4 and d5 ) after establishment of seizure model.The escape latency was significantly shorter for rats of the HRS group than for RS group rats at d4.③The level of Bcl-2 protein in the hippocampus was much lower in the RS group than in the HRS and control groups.In addition,the expression of PRG-1 in the RS group was significantly higher than in the CON group. ConclusionsRecurrent prolonged seizures cause long-term neurobehavior deficits,which might be associated with the down-regulated expression of Bcl-2 and up-regulated expression of PRG-1 in the hippocampus.Bubble baths can improve the neurobehavioral sequelae from seizures,perhaps through up-regulation of hippocampal Bcl-2 expression.

ObjectiveTo explore the intervention effect and the underlying molecular mechanism of 3-Methyladenine on behavioral damage of neonatal rat with prolonged seizures. MethodsForty-five 6-dayold SD rats were randomly ( random number) divided into the recurrent prolonged neonatal seizure group ( RS group), the 3-MA-treated seizure group and control group. The volatile agent flurothyl was used to induce 30 min seizure attack. At postnatal day 6 (P6), recurrent seizures were induced once daily for successive 6 days in both RS group and 3-MA group. In 3-MA group, 3-MA (2 μL) was injected daily before seizures induced.Neural-behavior changes were observed with double-blind method including swimming development, open field test and Morris water maze analysis. Bcl-2 and Beclinl protein levels in hippocampus were detected by western blot method at P50. ResultsThe total scores of swimming behavior in RS rats were decreased significantly compared with those of control and 3-MA rats ( control: 7. 44 ±1. 13, RS: 5.06±1.63, 3-MA: 7.33 ±1.08, F=16.19, P＜0. 01) . The start-latency time of open filed behavior in RS rats ( 13. 33 ±6. 69) were increased significantly compared with that of control (7. 11 ±2. 37) and 3-MA rats (9. 91 ±4. 23) (F=4. 39, P＜0. 05). The escape latency was significantly longer in rats of RS group than that of control and 3-MA rats on the 4th and 5th days (P ＜ 0.05). The level of Bcl-2 in hippocampus of RS group (0. 587 +0. 139) were significantly lower than that of control (0. 782 +0. 083) and 3-MA groups (0. 799 + 0. 163) (F =4. 7 1, P ＜ 0. 05 ). There were no significant differences in the level of Beclin1 protein in hippocampus among the three groups ( F =0. 27, P ＞ 0. 05 ).Conclusions Pretreatment with autophagy inhibitor 3-MA in acute phase of neonatal seizures could significantly improve neurobehavioral capacity, which might be associated with the increased in the level of Bcl-2 protein in hippocampus.

ObjectiveTo explore the potentiality of protection by bubble bath hydrotherapy to brain damage of neonatal rats with recurrent seizures.MethodsSixty Sprague-Dawley neonatal rats were randomly divided into control group ( CON),control hydrotherapy group ( HCON ),recurrent-seizure group (RS) and recurrent-seizure hydrotherapy group (HRS).Each group had 15 rats.The volatile agent flurothyl was used to induce 30 min seizure attack.At postnatal day 6 (P6),recurrent seizures were induced once per day for consecutive 6 days in the RS group and HRS group.Rats in the CON and HCON group were placed into the container for same time as their counterpart without exposure to flurothyl.In HCON and HRS groups,Bubble bath hydrotherapy intervention was used after the end of the last seizure for 28 consecutive days.Inspections were made for maturation of physical characteristics,neural behavior.PRG-1 and Bcl-2 protein level in cerebral cortex were detected by western blot method at P50.Results1.Maturation of physical characteristics:body weights of the rats in the RS group were decreased significantly (P＜0.05).Body weights of HRS rats were significantly increased than that of RS rats(P＜0.05 ).There were no significant difference of physiological development phenomenon among the five groups (P ＞0.05 ).2.There was high nerve excitation in hydrotherapy group ( At P26,the scores of OFT respectively were 45.78 ± 18.31 for CON,59.78 ± 25.61 for HCON,27.78 ± 16.83 for RS and 53.15 ± 22.43 for HRS,P ＜ 0.01 ).3.The expression of PRG-1 in cerebral cortex of RS group were significantly higher than that of CON group (1.149 ±0.191 for RS,0.910 ±0.162 for CON,P＜ 0.05 ).The level of Bcl-2 in cerebral cortex of RS group ( 1.149 ±0.191 ) were significantly lower than that of CON group( 1.259 ±0.157) (P＜0.05 ),the level of Bcl-2in cerebral cortex of HRS group( 1.572 ± 0.333 ) were significantly higher than that of RS group(P＜ 0.05 ).ConclusionMaturation of physical characteristics and neurobehavioral were damages following recurrent neonatal seizures,which may be associated with significantly increased level of PRG-1 protein and decreased level of Bcl-2protein in cerebral cortex.The early intervention by Bubble bath hydrotherapy can play a positive role on developmental rehabilitation.

Objective To compare the effects of hypothermic arrest circulation ( HAC) with moderate or deep hypothermic antegrade selective cerebral perfusion ( ASCP) technique in patients with DeBake type Ⅰ aortic dissection or aortic arch aneurysm undergoing total aortic arch replacement. Methods Between Feb. 2007 and Aug. 2009, 60 patients who underwent aortic arch replacement using HAC + SCP technique. Patients were divided into two groups according to the lowest nasopharyngeal temperature: moderate hypothermia (18 - 20℃) (group MH, n = 28), and deep hypothermia (23 -25 ℃) (group DH,n=32). The clinical and neurological outcomes were observed. Results The overall in-hospital mortality was 3. 33% (2/60) ; In-hospital mortality was 3. 6% in the MH group, while3.1% in the DH group. There were no significant differences regarding to preoperative the patients' character parameters in both groups. At the beginning of ASCP, the nasopharyngeal and rectal temperatures were significantly higher in group MH[MH group (23.8 ± 1. 9 ) ℃ ( 26. 6 ± 2. 4 ) ℃ vs. DH group group (29.4 ±9.3) min vs. DH group(32.9 ± 13.4) min]. The rewarming time and CPB time were significantly shorter in MH group[(65.0±13.9) min vs. (90.3 ±27.2) min; (142.8 ±34.2) min vs. (194.1 ±42.0) min, P <0. 05]. Temporary neurologic deficits occurred in four patients (6. 67% ) without significant differences between two groups ( MH group 7.14% vs. DH group 6. 25% ). No patient suffered from permanent neurologic dysfunction. There were no significant differences between two groups in other parameters including intubation time, kidney insufficiency, the stay times in ICU and hospital. Conclusion Our results demonstrate that moderate HCA + ASCP technique is a safe strategy for the treatment of patients who require conventional total arch replacement with individual arch-vessel reconstruction. Shorter CPB time in patients with moderate HCA + ASCP did not increase any other postoperative adverse effects.

Objective To analyze the expression of prolactin protein and ZnT1 mRNA in rat brain in the wake of heat stress and febrile convulsion(FC).Method Thirty-six weanling Spragne-Dawley rats were randomly divided into control group(n=8) and other 28 rats were used to induce heat stroke by warm water but three of them failed to produc expected heat stress.Consequently,there were 35 rats eligible to be models of heat stroke,and of them,10 rats showed heat stress(HS group,n=10) and 15 rats had 5-grade febrile convulsion (FC group,n = 15).The inmmunohistocbemistry and in situ hybridization method were used in this study.The IR) neurons were found in the rats of control group.The deep immune staining was found in the PIR,Era and RS regions of cerebral cortex and light immune staining was found in the PRH,PAR and FR regions of cerebral cortex in HS group.In addition,the PRL-IR positive neurons were found around the midline strip of thalamus without characteristic subnucleus-specific distribution.However,abundant induction of PRL-IR positive neurons with diffuse distribution were found in cerebral cortex,hippoeampus,amygdala,thalamus and hypotha]amns of rats in FC and FC rats showed more PRD-IR positive neurons in cerebral cortex than those in rats of control group (P

To guarantee the safe and reliable operation of its information system, our hospital, in light of the features of HIS, came up with a feasible design plan for the server system. The paper discusses the fault tolerant technique, the key technique involved in the plan, explains the mechanism of the ROSE HA fault tolerant software, analyses the implementation method of the RAID technique and the application of the disc array, describes the operating principles of the VERITAS Backup EXEC software, introduces the on line backup method and strategy for the ORACLE database, and illustrates the implementation method of the banking of the central servers dual systems and the configuration of relevant hardware.

Objective　To analyse the state of the administration of clinical med icine in some children's hospital. Methods　Prevalence study was use d. Results　The administration of antibiotic and vitamin were domina nt among inpatients, and their utilization rates were 84.26%, 79.08% respec tively. 1 643 among 1950 of cases used antibiotic agents, the utilization rates of penicil ins, cephalosporins and antiviral medicine were 53.38%, 31.21% and 30.74% respec tiv ely. The administration of combined antibiotic added up to 71.88%. Conclusions　The phenomenon of irrational administration of medicine wa s existing in the children's hospital. So it should be perfected on the rules an d regulations for the administration of medicine, and the management of medicine should be strengthened.

Objective　 To promote the therapeutic effect about the primary hypertension connected psychological factor through interventing anxiety emotion.Method　All primary hypertension patients with anxiety emotion were divided into two groups randomly.Each group was treated by lotension for 8 weeks,While group 1 combined antianxiety drugs Deanxit Result　The effective rate of two groups were 93.94%(group 1), 60.61%(group 2).Conclusion　 The therapeutic effect of group 2 is lower than that of group 1.

Objective To explore the application of adopting the solid phase adsorption method to extract serum hepatitis C virus (HCV) RNA in the real-time fluorescence quantification PCR( RT-qPCR) by the improvement of solid phase adsorption method . Methods Firstly ,the surface of silica nano-particles was modified with Si-OH for increasing their adsorption ability with HCV RNA .Then the concentration of guanidinium thiocyanate (GuSCN) in the viral lysate was optimized .Thirdly ,the HCV RNA of standard serum samples was extracted using the modified solid-phase adsorption method and then performed in the RT-qPCR .Its standard curve was drawn and the linear equation was calculated .At the same time ,the lowest detection limit of RT-qPCR for de-tecting HCVRNA in serum sample was measured and the detection repeatability was verified .Results The adsorption ability of sil-ica nano-particles for HCV RNA in serum samples was remarkably improved after surface modification with Si-OH .The optimal GuSCN concentration in viral lysates was 4 .23 mol/L .Using the modified method to extract HCV RNA in serum samples was ap-plied in RT-qPCR ,the standard curve and the linear equation were obtained ,the linear correlation coefficient(R2) reached to 0 .999 and the detected linear range was (2 .52 -5 .52) IU/mL or 102 .52 -105 .52 pathogen number/mL .The detection limit of HCV RNA in serum samples was (2 .52 ± 0 .50)IU/mL with good detection repeatability .Conclusion The modified solid-phase adsorp-tion method can greatly decrease the lowest detection limit of RT-qPCR and increase the positive detection rate of HCV .This meth-od is simple to operate ,has low cost and can be widely applied in the HCV detection and the nucleic acid detection of other viruses in clinic .

Objective To explore the intervention effect of cathepsin B inhibitor (CBI) and its signaling pathways after flurothyl inducing brain injury in rats with recurrent seizures. Methods 6-day-old (P6) SD rats were randomly divided into: recurrent neonatal seizure group ( RS group, n = 30), CBI-treated seizure group ( CBI group, n=30) and the control group( CONT group). Rats in RS group were subjected to 5 seizures with flurothyl during the first 14 days of life. In CBI group,CBI was injected each day before seizures were induced. Then examined development indexes such as the physical growth, neural reflex, neural behavior and cognitive emotional competence. Western blot was employed to determine Cathepsin B expression at different time points ( 1.5h,3h,6h,24h and P35) after the last convulsion. Results The weights of rats in RS group( (27.28 ± 1.6) kg) were lighter than CONT group( ( 33.45 ± 1.57 ) kg). They had significant difference (P＜ 0.01 ) at the age of p14. The time of cliff avoidance in RS group( (2. 10 ± 1.45 ) s) was longer than CBI group( ( 1.05 ± 0. 32) s). It showed a statistically significant (P ＜ 0.05 ) in p12. In the open-field behavior test: the activities of RS group (20.00 ± 13.08 )were markedly reduced than CONT group ( 57.83 ± 33.22 ) in the horizontal movements, the RS group ( 2.50 ±2.43 ) were significantly decreased than the CONT group and CB1 group( ( 22.17 ± 10.74), (9.33 ± 5.39 ) ) in the vertical motions (P ＜ 0. 05 ). Cathepsin-B expression in RS group( 1.5h, 3h,6h and 24h )was significantly higher than that at the same time in CONT group(P＜ 0. 05 ). Cathepsin-B expression of CBI group was significantly decreased compared with that in RS group (P＜ 0. 05 ) at the time point (1.5h ,3h,6h and 24h). There were no significant differences among three groups at P35(P＞0.05 ). Conclusions CBI can improve brain injury and regulate the expression of abnormal molecules.