Objective:To image workstations in the necessity of desktop computer and data lines are connected and fixed in the desktop computer of defects, design a set of wireless transmission of picture archiving and communication system.Methods: Notebook computer with a wireless network using a wireless router, switch and set up a set of wireless image text transmission equipment.Results:The wireless transmission of honeycomb like structure covered with 802.11n standard data rates up to 600Mbps, frequency of 5Hz, multiple security protection by the hospital image filing requirements, which makes medical information resources to achieve full sharing. Conclusion: In 802.11n Wireless LAN PACS system architecture, the basic realization of the wireless transmission and real-time image display, image data for clinicians can within the hospital patients with real time view and its important role in the future development of hospital.

OBJECTIVE: To study the effects of extracts of the seed of Litchi (ESL) on the expression of estradiol receptor (ER) and progestogen receptor (PR) in mammary tissue and apoptosis of mammary glandular cells in rats with hyperplasia of mammary glands (HMG). METHODS: The HMG model was induced by intramuscularly injecting with 0.5 mg?kg-1 estradiol benzoate for 25 days, subsequently intramuscularly injecting with 4 mg?kg-1 progesterone for 5 days. 60 healthy nonfertile SD rats were divided into normal group, model group, rujieping group and high-dose, medium-dose and low-dose ESL groups, treating with distilled water, distilled water, 3.3 g?kg-1 Rujieping and 44, 22, 11 g?kg-1 ESL via i.g. gtt once a day for 30 days, respectively. Immunohistochemical method was applied to observe the expression of ER and PR in rat mammary tissue and apoptosis of mammary glandular cells. Uterus index was also calculated. RESULTS: High-dose and medium-dose of ESL can inhibit the expression of ER and PR of mammary gland in HMG rats and induce apoptosis of mammary glandular cells(P

Objective:To explore therapeutic effects of trimetazidine combined atorvastatin calcium on chronic heart failure (CHF) .Methods:According to random number table ,a total of 80 CHF patients were randomly and equally divided into routine treatment group and combined treatment group (received trimetazidine and atorvastatin calcium based on routine treatment group) .NYHA class improvement ,6min walking distance (6MWD) ,left ventricular ejection fraction (LVEF) , left ventricular end‐systolic dimension (LVESd ) , plasma levels of brain natriuretic peptide (BNP ) , C reactive protein (CRP) and adverse drug reactions (ADR) were compared between two groups before and six months after treatment .Re‐sults:Compared with routine treatment group after treatment ,there was significant rise in total effective rate of heart function improvement (77.5% vs .95.0% ) in combined treatment group , P=0.023. Compared with routine treatment group ,there were significant rise in 6MWD [ (346.6 ± 47.3) m vs .(396.4 ± 49.2) m] and LVEF [ (44.5 ± 4.8)% vs . (49.3 ± 5.4)% ] ,and significant reductions in LVESd [ (54.2 ± 4.1) mm vs .(51.1 ± 4.3) mm] ,plasma levels of BNP [ (348.4 ± 61.3) pg/ml vs .(251.4 ± 53.6) pg/ml] and CRP [ (9.1 ± 2.2) mg/L vs .(6.4 ± 1.6) mg/L] in combined treatment group , P<0.05 or <0.01. No severe ADR was found in two groups .Conclusion:Trimetazidine combined ator‐vastatin calcium can significantly improve cardiac function and exercise capacity ,improve quality of life and ventricular re‐modeling in CHF patients ,it's safe and reliable ,which is worth extending .

Being a sensitive,accurate,non-invasive and simple method,the H_2 breath test (HBT) has been widely used in determining intestinal absorption,intestinal motor function,ex- cessive bacterial growth in the intestinal aad gastric secretion.The key technique in performing HBT is the measurement of the pauci-hydrogen in expiratory air.We have successfully developed the type-88 H_2 breath tester.According to the results of performance evaluated by the Natienal Standard Material Research Center and the reports of its clincal application,it is superior in both design and performance.This new tester is suitable for use in clinics and field work owing to its high performance and simplicity in operation.

The content of merthiolate in intramuscular immunoglobulin was determined using cold vapour atomic absorption method after its sample-had been ingested by 5%KMnO_4 and strong H_2SO_4 in the boiled water bath for 30 minutes. And the correlation coefficient was 0.9992, the average recovery was 99.7～104%, the CV rate was 0.9～3.8%. The results of experiment were accurate and reliable as compared with traditional diphenyl thiocarbazone titration, indicating that the method was simple, quick and worthy to be spread.(Original article on page12)

It is very important for the research nurses to announce the purpose, to prepare and transfuse the agents, to observe the therapic effects and to collect the data in clinical trials of nutrition agents. Our experiences include: ①The research knowledge of observing nurses must be trained. ②The management system should be standard and specific. ③The health education should be careful. ④The dose of testing agents must be precise. ⑤The sample collection must be correct.

The portable dynamic electrocardiosignal data collector is the important part in the dynamic ECG analysis system.In this paper,the writer introduces the principle of intelligent data collector system,which takes microcontroller as core,and discusses the points of hardware design and the arithmatic of data pretreatment software.

Objective To investigate the resistance of clinical isolates of P.aeruginosa in China during 2005.Methods Clinical isolates of P.aeruginosa from 7 teaching hospitals in China were collected and antimicrobial susceptibility were tested by Kirby-Bauer method.Results A total of 2 323 clinical isolates of P.aeruginosa were collected.The resistance rates of P.aeruginosa to antimicrobial agents from low to high were amikacin(22.5%),cefoperazone-sulbactam(22.8%),cefepime(27.6%),ceftazidime(28.9%),imipenem(31.3%),ciprofloxacin(31.7%),meropenem(33.7%),piperacillin-tazobactam(34.4%),aztreonam(36.8%),piperacillin(44.2%),ticarcillin-clavulanic acid(50.9%).Multi-drug resistant(MDR)and pan-drug resistant(PDR)strains accounted for 8.4% and 4.2%,respectively.Some(36.7%-53.4%)of the strains resistant to other antimicrobial agents were still susceptible to amikacin.Therefore,combination therapy of ?-lactams plus an aminoglycoside is usually indicated for serious infections caused by P.aeruginosa.By 'interpretative reading' of the susceptibility pattern of 'predictive drugs',we can predict the underlying resistance mechanisms.Conclusions The surveillance data indicate that the resistance of P.aeruginosa in China is relatively serious and its resistance mechanisms are very complicated.Close attention should be paid to this problem by physicians and clinical laboratory.

Objective: To obtain highly purified recombinant CP4-EPSPS antigen with well reserved original antigenicity by a simple and fast protocol,and to study immunoactivity of recombinant CP4 EPSPS antigen.Methods: Recombinant expression vector pET-EPSPS containing CP4-EPSPS gene from transgenetic soy bean was induced by optional concentration of IPTG.After antigen activity was identified by strip-test method,recombinant protein expressed in the supernatant was purified by Ni-NTA-chelated agarose affinity chromatography method.Immunoactivity of purified CP4-EPSPS was further confirmed by ELISA assay,strip-test method,and Western blot analysis.Results: Recombinant CP4-EPSPS antigen was mainly expressed in the form of inclusion bodies in E.Coli system,whereas the strong antigenicity of CP4-EPSPS was detected in the induced supernatant by Colloidal-Gold strip-test,no antigenicity of CP4-EPSPS could be detected by Colloidal-Gold strip test for inclusion bodies or renatured sample from inclusion bodies.CP4-EPSPS protein was successfully purified from induced supernatant via propagating host cell culture and reducing the re-suspension volume of induced culture.ELISA assay indicated the specific antibody titer of recombiant antigen-immunized serum sample could reach 1:625,000 after three months of immunization.Using serum sample derived from recombinant antigen-immunized mice as primary antibody,CP4-EPSPS standard and purified recombinant antigen showed a highly specific and strong reaction with this serum by Western blot analysis.Conclusion: The original antigenicity of CP4-EPSPS cannot be recovered completely during gradual renaturing procedures by simple dilution methods.The epitope related to the rapid detection of CP4-EPSPS cannot be recovered by renaturing the inclusion bodies by the method in this study.However,CP4-EPSPS in induced supernatant is found to have a well-reserved original antigenicity.Colloidal Gold Strip-test to detect recombinantCP4-EPSPS antigen is a highly sensitive,and convenient method to follow through the whole process of recombinant expression and purification.It plays a crucial role in identifying the induced recombinant CP4-EPSPS for purification and further application in the immunization for the monoclonal antibody development.

Objective:To investigate the effect of cable pin internalfixation for treatment of patellar fracture.Methods:12 cases with patellar fracture were treated by cable pin internalfixation.Results:The post-operative function in 12 cases was evaluated according to Lu Yu-pu score,follow-up averaged 10 months,and the results were :excellent in 9 cases(75%);good in 2 cases(16.7%);fair in 1 cases(8.3%);poor in 0 case.The excellent rate was 91.7%.Conclusion:Patellar fracture treated with cable pin has the advantages of simple manipulation,firm fixation,faster and guaranteed fracture heeling and good function of knee.The method meets the demands of biomechanics and the clinical result of this group is quite satisfactory.It is advisable to use it in clinical practice.