OBJECTIVE: To study the effects of Semen Litchi on the expressions of S180 and EAC tumor cells and Bax and Bcl-2 proteins in rats. METHODS: EAC, S180 and hepatic carcinoma tumor-bearing mice models were established in mice by inoculating them with tumor strains. EAC, S180 and hepatic carcinoma suspensions were diluted respectively with normal saline in a ratio of 1∶1 for the preparation of tumor-bearing ascites suspension. Each rat was subcutaneously inoculated with the mixture 0.2 mL via axilla 24 hours before medication, with the whole sterile operation finished within 60 minutes. After treated with Semen Litchi for 10 consecutive days, the growth of EAC, S180 and hepatic carcinoma in mice was observed. RESULTS: The growth of EAC and S180 and hepatic carcinoma cells in mice was significantly inhibited by Semen Litchi. CONCLUSION: Semen Litchi can inhibit the growth of S180 and EAC tumor cells in vitro and in vivo, and its antitumor effect is related to its ability to promote apoptotic of tumor cells.

OBJECTIVE: To study the effects of extracts of the seed of Litchi (ESL) on the expression of estradiol receptor (ER) and progestogen receptor (PR) in mammary tissue and apoptosis of mammary glandular cells in rats with hyperplasia of mammary glands (HMG). METHODS: The HMG model was induced by intramuscularly injecting with 0.5 mg?kg-1 estradiol benzoate for 25 days, subsequently intramuscularly injecting with 4 mg?kg-1 progesterone for 5 days. 60 healthy nonfertile SD rats were divided into normal group, model group, rujieping group and high-dose, medium-dose and low-dose ESL groups, treating with distilled water, distilled water, 3.3 g?kg-1 Rujieping and 44, 22, 11 g?kg-1 ESL via i.g. gtt once a day for 30 days, respectively. Immunohistochemical method was applied to observe the expression of ER and PR in rat mammary tissue and apoptosis of mammary glandular cells. Uterus index was also calculated. RESULTS: High-dose and medium-dose of ESL can inhibit the expression of ER and PR of mammary gland in HMG rats and induce apoptosis of mammary glandular cells(P

Objective: To investigate the effects of devascularization procedure on portal hemodynamics and recent and long-term hepatic function of portal hypertensive patients. Methods: A retrospective clinical analysis about portal hemodynamics and recent and long-term hepatic function was made in 367 cases of portal hypertensive patients, who admitted to our hospital and subjected to double devascularization from January 1989 to January 2008. Results: It was found that FPP was decreased from (36.2?4.2)cmH2O preoperatively to (31.4?3.3)cmH2O postoperatively (P0.05), but if was improved significantly after 6 and 12 month (P

Thirty seven cases after myocardial infarction, in which 15 cases previously had ST reciprocal changes (ST-RC) and 22 cases without that changes (without ST-RC) in acute phase. Bicycle ergometer exercise test was used to osbserve the clinical significance about ST-RC patients. The results are as follows. 1 ) ST-RC group have induced angina pectoris, ST segments depression in reciprocal region of infarcted areas and positive results with exercise test all are significantly higher than that of non-ST-RC group(P

Objective To explore the effect of naphthalene acetic acid(NAA) on animal cells. Methods K562 cells were treated in vitro with different doses of NAA (0, 50 and 100?mol/L). The cell proliferation, cell cycle, cell division index and apoptosis was observed by means of MTT, cell enumeration, and flow cytometry. The sister chromatid exchange (SCE) and micronucleus were tested. Results The proliferation of K562 cell was inhibited by NAA compared with that of the controls; the cell cycle was arrested at G1, and the cell division index reduced; apoptosis cells increased lightly in NAA experiments; the frequency of SCE and micronuclear formation were increased by NAA treatment. Conclusion NAA has antiproliferative activities on K562 cell.

In order to provide highly purified preparation of human ieukoregulin (LR) for the exploration of its anticancer mschatiism, crude preparations of LR and lymphotoxin(LT) derived from human spleen cell cultures induced with phytohem-agglutinin and diterp;ne ester l2-O-tetradecanoylphorbol-i3-acetate were purified by using four purification nmhois : (a) Con A-Sepharose 48 affinity chromatography, by which crude human LR or LT was purified to maximum specific activity of 85 333 U/mg protein or 13333 U/mg protein with a recovery of 28.1% or 31.9%, respectively 5 (b) DEAE-Sjphadex A-50 ion-exchange chromatography, by which partially purified LR or LT obtained after Con A-Sepharose 4B affinity chromatography was further purified 2.44-fold or 3.8o-fold with a recovery of 96% or 92%, respectively ; (c) DEAE-22-celluiose ion-exchange chromatography, by which crude human LR or LT was purified to maximum specific activity of 11 294 U/mg protein or 13 176 U/mg protein with a recovery of 87.5% or 89.6%.respectively ; (d) Blue-Sepharose CL-6B affinity chromatography, by which crude human LR or LT was purified to maximum specific activity of 51.89 U/mg protein or 2594 U/mg protein with a recovery of 53.75% or 5t3.G7%, respectively.Our data indicate that Con A-Sepharose 4B affinity chromatography, DEAE-Sephadex A-50 ion-exchange chromatography.DEAE-22-cellulose ion-exchange chromatography and Blue-Sepharose CL-6B affinity chromatography may be useful in the purification of LR or LT.

The quantitative determination of octanoate content in albumin product was performed by acid base titration directly after the water phase was mixed up with the ether phase using the mixture solvent solution. The three factor optimum seeking principle was used to seek the optimum concentrations and volumes of components in mixture solvent solution and the optimum conditions of extraction. The method had its recovery rate of 99. 8+0. 89%(n=6) and variation coeff-cient (CV)of 0. 89%(n= 16),indicating that the method was a precise and reliable one.

Intestinal trefoil factor(ITF)is a low molecular weight polypeptide expressed in intestine. ITF is also found in the brain,respiratory,eyes and so on.To study recombinant ITF will have an important impact for the treatment of gastrointestinal disease, respiratory tract disease, eyes disease and so on.

Objective:To investigate the effect of cable pin internalfixation for treatment of patellar fracture.Methods:12 cases with patellar fracture were treated by cable pin internalfixation.Results:The post-operative function in 12 cases was evaluated according to Lu Yu-pu score,follow-up averaged 10 months,and the results were :excellent in 9 cases(75%);good in 2 cases(16.7%);fair in 1 cases(8.3%);poor in 0 case.The excellent rate was 91.7%.Conclusion:Patellar fracture treated with cable pin has the advantages of simple manipulation,firm fixation,faster and guaranteed fracture heeling and good function of knee.The method meets the demands of biomechanics and the clinical result of this group is quite satisfactory.It is advisable to use it in clinical practice.

Objective To explore the value of laparoscopy in the treatment of ovarian cyst.Methods The clinical datas of 48 cases with laparoscopic surgery were retrospectively analyzed,its safety and possibility were explored.Results Forty-eight patients with ovarian cyst underwent laparoscopic oophorocystectomy.The average blood loss during operation was 80ml,the mean operation was 55 minutes,the mean hospitalization was 4 days.No one converted to open prodecure,no visceral injured,no chemical peritonitis occurred.Conclusion If the indications are well selected,laparoscopic operation is feasible and safe in treating most ovarian cysts.It may be a kind of ideal type of treatment for the clinic to choose.