AIM　To investigate the chemical constituents of Rubus chroosepalus Focke. METHODS The methanol extracts were suspended in H2O and then extracted with EtOAc. Column chromatography was used for separation and purification, while spectral analysis was used for identification. RESULTS　Seven compounds were isolated and identified as 2α,3β-dihydroxy-urs-12,19-dien-23,28-oic acid (I), 2α,3β,23-trihydroxy-urs-12,18-dien-28-oic acid (IIa), 2α,3β,23-trihydroxy-urs-12,19-dien-28-oic acid (IIb), 2α,3α-dihydroxy-urs-12,18-dien-28-oic acid (IIIa), 2α,3α-dihydroxy-urs-12,19-dien-28-oic acid (IIIb), and the acetonide of IIIa and IIIb (IVa and IVb). CONCLUSION　I was found to be a new pentacyclic triterpenoid acid.

Object To investigate the chemical constituents from the methanol extract of the root of Rubus irenaeus Focke. Methods EtOAc extraction, normal and reverse phase silica gel column chromatography were used for isolation. Spectroscopic methods ( 13 CNMR , 1HNMR , DEPT, 2DNMR, and FAB-MS) and comparison with authentic samples were used for identification. Results Ten compounds were isoated and characterized as 2?, 19?-dihydroxyl-3-oxo-urs-12-en-28-oic acid (Ⅰ), 2-oxo-pomolic acid (Ⅱ), fupenzic acid (Ⅲ), euscaphic acid (Ⅳ), 2?, 3?, 19?-trihydroxyl-olean-12-en-28-oic acid (Ⅴ), 2, 3-O-isopropylidenyl euscaphic acid (Ⅵ), pomolic acid (Ⅶ), 2?, 3?-dihydroxyl-lup-20(29)-en-28-oic acid(Ⅷ), catechin (Ⅸ) and daucosterol (Ⅹ). Conclusion These compounds were obtained from this plant for the first time.