OBJECTIVE:To establish a method for the determination of the bacterial endotoxin content in isepamicin sulfate injection. METHODS: By using tinetic turbidimetric assay in which the sample was subjected to a serial dilution and the detection concentration of the sample was established by interference tests, meanwhile the standard endotoxin working curve was established for quantitative determination of the bacterial endotoxin in samples. RESULTS: The results showed that at a concentration of 6.25 mg?mL-1, isepamicin sulfate injection did no interference on tachypleus amebocyte lysate test. The bacterial endotoxin recovery ranged form 50% to 200%. CONCLUSIONS: It is feasible to use kinetic turbidimetry assay for the determination of bacterial endotoxin in isepamicin sulfate injection.

Objective: To detect the thickness of silicon coating film for glass injection bottles by focused ion beam (FIB) emission scanning electron microscopy and investigate the stability and uniformity of the film.Methods: The boron silicon coating injection bottles were selected as the experimental samples and treated with such stability experiments as ultrasonic cleaning, high temperature resistance, heat resistance, water resistance, acid resistance and alkali resistance.The samples were vertically cut by the ion beam from an FIB field emission scanning electron microscope, and the thickness of the cutting face was measured by the built-in measuring module of the scanning electron microscope.Results: The thickness of silicon film was stable and uniform.Conclusion: The film thickness of glass bottles can be accurately detected, and the film stability and uniformity can be reflected directly and objectively by using an FIB double beam field emission scanning electron microscope.

Objective To investigate the expression changes and significance of Pdlim2 in the glomerular podocyte of hyperlipidemic rats.Methods Forty-five individuals of SD rats were divided randomly into 3 groups (n =15 in each group).The control group was fed with normal diet.The high fat group was fed with high fat diet.The simvastatin group was fed with high fat diet plus with simvastatin gavage (10 mg· kg-1 · d-1).Five rats were sampled randomly from each group at week 4,6,and 10 and the urinary protein excretion,the concentration of serum cholesterol,and the concentration of low density lipoprotein cholestorol were determined,the glomerular podocyte damage in rats was detected by electron microscope,the expression of Pdlim2 protein was determined by immunohistochemistry and by Western blotting.Results The levels of serum cholesterol and low density lipoprotein cholestorol increased significantly in high fat group and simvastatin group at week 4 compared to that in control group(P ＜ 0.05),and the level in simvastatin group was significantly decreased compared with that in high fat group(P ＜ 0.05).The urinary protein levels of high fat group and simvastatin group were significantly higher than that in control group at week 10,and the level in simvastatin group was significantly decreased in high fat group,there was significant difference in each group of comparison(P ＜ 0.05).Podocyte injury was detected by electronic microscopy in high fat group at week 4,and the injury became more serious as the treatment time increased.Podocyte injury in the simvastatin group was significantly less than that in the high fat group and the control group at week 10.The positive staining of Pdlim2 was mainly in the glomeruli and the expression of Pdlim2 of the high fat group was lower than the simvastatin group,and both were lower than the control group at week 10.The expression of Pdlim2 protein of high fat group was lower than that in the control group since week 4(P ＜0.05).The expression of Pdlim2 protein of high fat group was lower than that in the simvastatin group (P＜0.05),and both were lower than the control group at week 10(P＜0.05).Conclusions Hypedipidemia induces podocyte injury before urinary protein,which is suggested to be associated with the decrease of Pdlim2 protein.Simvastatin reduces podocyte toot processes of high fat induced fusion,which may be through protecting the expression of glomerular Pdlim2.

Objective:To investigate the effects of two drugs on the asphyxia oxygen concentration and the oxygen consumption rate of zebrafish,and explore the possibility of zebrafish as a drug model and the feasibility of oxygen microelectrode used for drug screening. Methods:The zebrafish were treated with the drugs for 5 days,and transferred into the asphyxia chambers. The solute oxy-gen concentration was assessed continuously until the asphyxia condition. The asphyxia oxygen concentration,asphyxia time and mass specific oxygen consumption rate were determined. Results:The results showed that the asphyxia time,asphyxia concentration and ox-ygen consumption rate of the zebrafish were not significantly changed by deproteinized calfblood extractives injection(P>0.05),sug-gesting no reduction in hypoxia tolerance ability. The asphyxia time and oxygen consumption rate of the zebrafish were not significantly changed,either (P>0.05),however,the asphyxia concentration was increased significantly by cervus and cucumis polypeptide injec-tion (P<0.05),suggesting the reduction in hypoxia tolerance ability. Conclusion:It is suggested that the hypoxia tolerance ability of zebrafish can be significantly decreased by the solution of cervus and cucumis polypeptide. The results also suggest that zebrafish can be used as an experimental animal in medicine studies,and the oxygen microelectrode method can be used in drug screening.