1.Protective effect of sodium and magnesium saline of fructose-1,6-diphosphate on B cells in pancreatic islet injured by streptozotocin
Journal of Third Military Medical University 2003;0(24):-
Objective To explore whether sodium and magnesium saline of fructose-1,6-diphosphate(Na +-Mg 2+-FDP)protects B cells in islet of Langerhans injured by streptozotocin(STZ).Methods Healthy adult male Wistar rats were randomly divided into five groups:STZ group(receiving once 2% STZ in citric acid solution at dose of 60 mg/kg),blank control group(receiving citric acid solution),FDP group(treated as STZ group,plus Na +-Mg 2+-FDP solution twice a day at total dose of 500 mg?kg-1?d-1 for 15 d),protected group(receiving Na +-Mg 2+-FDP solution at 500 mg/kg first,then 2% STZ in citric acid solution at dose of 60 mg/kg once,Na +-Mg 2+-FDP solution twice a day at total dose of 500 mg?kg-1?d-1 for 15 d),treated group(48 h after receiving 2% STZ in citric acid solution at dose of 60 mg/kg,starting Na +-Mg 2+-FDP solution twice a day at total dose of 500 mg?kg-1?d-1 for 15 d).On the 15 th day after experiment starting,all rats were killed and the end of pancreatic gland was prepared for following experiments.Blood glucose by using glucose oxidase kit and serum insulin by radioimmunoassay was determined.The number of positive islets of which A,B and D cells expressed glucagons,insulin and somatostatin was counted by S-P immunohistochemistry.The morphology of islet of Langerhans stained by HE was observed under optical microscope.Statistical significance was determined by q test and ? 2 test,the related coefficient was determined by rectilinear correlation analysis(?=0.05).Results The blood glucose dropped and serum insulin rose in protected group,but the glucose in treated group and STZ group maintained at high level,and serum insulin maintained at low level.The blood glucose and insulin of the protected group showed significant differences with that of the treated group and STZ group respectively(P0.05).However,the expressions of insulin,gluca-gons and somatostatin were close to normal level in protected group,which were of statistical significance with that of STZ group and treated group(P
2.Essential Oil from Alpiniae Zerumbet Fructus Alleviates High Glucose-induced HUVEC Injury via TXNIP Signaling Pathway
Wen ZHANG ; Nen-ling ZHANG ; Yan-yan ZHANG ; Yan CHEN ; Ling-yun FU ; Di PAN ; Xiang-chun SHEN
Chinese Journal of Experimental Traditional Medical Formulae 2021;27(13):21-27
Objective:To study the protective effect of essential oil from Alpiniae Zerumbet Fructus (EOAZF) against high glucose (HG)-induced injury of human umbilical vein endothelial cells (HUVECs)
3.Synthesis and cytotoxicity of longistylin C derivatives.
Yan SHAN ; Ting HONG ; Yan-Fei WANG ; Nen-Ling ZHANG ; Bo YU ; Yu LU ; Sheng-Xiang QIU
Chinese Journal of Natural Medicines (English Ed.) 2015;13(4):311-315
The present study was designed to identify potent anti-tumor compounds from a series of new longistylin C derivatives. Ten longistylin C derivatives were synthesized and their structures were confirmed by (1)H NMR, MS, and elemental analyses. Their cytotoxicity in vitro against three human cancer cell lines (A549, HepG2, and MCF-7) were evaluated by the MTT assay. Among these compounds, DT-6 and DT-9 displayed much better cytotoxicity against A549, HepG2, and MCF-7 cells, DT-1 exhibited selective cytotoxicity against HepG2, and the structure-activity relationships were investigated. In conclusion, Compounds DT-6 and DT-9 may serve as potential lead compounds for the discovery of new anti-cancer drugs.
Antineoplastic Agents
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chemical synthesis
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pharmacology
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Cell Line, Tumor
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Drug Screening Assays, Antitumor
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Humans
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Structure-Activity Relationship
4. Effect of Oxymatrine in Inhibiting Proliferation and Migration of HT-29 Cells Induced by Insulin
Di PAN ; Wen ZHANG ; Rong ZHANG ; Shi-quan GAN ; Yan CHEN ; Nen-ling ZHANG ; Yi-ni XU ; Xiang-chun SHEN
Chinese Journal of Experimental Traditional Medical Formulae 2019;25(24):36-42
Objective:To investigate the effect of oxymatrine (OMT) on the proliferation and migration of human colon cancer cell line HT-29 under Type Ⅱ diabetes environment by co-culturing HT-29 with insulin to simulate hyperinsulinemia. Method:The effect of OMT (2, 4, 8 mmol·L-1) on insulin-induced proliferation of HT-29 was detected by methyl thiazolyl tetrazolium (MTT) assay and cloning assay. The morphology change and cell migration were evaluated under microscope and by wound healing assay. The Annexin V/propidium iodide(PI) assay was used to detect the change of insulin-induced HT-29 cell cycle and apoptosis. Western blot was performed to validate the expression of cell cycle-related protein and cell migration protein. Result:Insulin significantly increased growth of HT-29 (P<0.05). Compared with insulin group, OMT with 2, 4, 8 mmol·L-1 showed a significant inhibitory effect in this model (P<0.05). In addition, OMT blocked HT-29 cell cycle in G0/G1 phase (P<0.05), and showed a slight apoptotic effect. Western blot showed that the down-regulation of Cyclin D1, CDK4 and the up-regulation of p27 by OMT might involve the growth inhibition mechanism. Furthermore, OMT reduced the migration of insulin-induced HT-29 according to wound healing assay(P<0.05). Decreased Vimentin (P<0.05)and increased E-cadherin(P<0.05)might be correlated with the migration restrain. Conclusion:OMT can inhibit the proliferation and migration of insulin-induced HT-29 cells. The changes of cell cycle and migration related proteins may be correlated with the mechanism.
5.Inhibitory Effect of Essential Oil from Fructus Alpinia zerumbet on Endothelial-to-mesenchymal Transition Induced by High Glucose via Nrf2/Notch1 Signaling Pathway
Shuang ZHAO ; Li HE ; Mei HUANG ; Nen-ling ZHANG ; Feng JIANG ; Xiang-chun SHEN ; Ling LI ; Yan-yan ZHANG
Chinese Journal of Experimental Traditional Medical Formulae 2020;26(23):99-105
Objective:To investigate the inhibitory effect and the possible mechanism of essential oil from fructus