The purpose of the study was to investigate the relationship between need satisfaction and mental well-being among university students in perspective of gender and job status. The sample was randomly selected from different universities of Islamabad, comprised of (N=160; n=80: working; n=40 males, n=40 females, n=80: n=40 males, n=40 females non-working) university students. Age range was 20 to 40. Basic need satisfaction scale was used to measure the satisfaction of basic needs and The Warwick-Edinburgh Mental Well-being Scale was used to assess the mental well-being. Results found that Basic Need Satisfaction and Mental Wellbeing has significant relationship (r=0.30, p=0.000) among university students. Results revealed that there was a significant difference of basic need satisfaction (t=-4.68, p<0.000) and mental wellbeing ((t=3.21, p<0.002) between male working and female working students. Female working students have high basic need satisfaction (M=88.93, SD=19.47) and Mental well-being (M=52.88, SD=9.14) than male working students. While no significant difference of Basic Need Satisfaction (t=0.64) and Mental Wellbeing (t=-1.31) among overall sample in perspective of gender and job status.