Introduction: Introduction: Knee valgus which also known as dynamic knee valgus (DKV), is frequently linked to non-contact lower-limb injuries, especially in females. This retrospective study aims to compare the kinematic variables of lower limb joints in physically active females with normal versus excessive DKV during single leg squat (SLS) at 45° and 60° knee flexion. Methods: Based on the outcomes of drop vertical jump screening test, 34 females were recruited and divided into two groups (i.e., normal and excessive DKV). Participants performed SLS at 45° and 60° knee flexion with three-dimensional motion capture and analysis. The kinematic variables of lower limb joints at both knee flexion of SLS were compared across groups using independent T-test. Results: During 45° SLS with the dominant limb, the normal DKV group performed significantly greater hip adduction angle (4.49±3.25°, t(32) = 2.371, p= 0.024) than the excessive DKV group (1.426±4.23°). During 60° SLS with the dominant limb, the normal DKV group showed knee adduction (0.223±0.07°, t(16.048) = 10.707, p=0.001) while the excessive DKV group showed knee abduction (-4.478±1.81°). Conclusion: Females with excessive DKV showed significantly different lower limb kinematics and motion control strategy compared to females with normal range of DKV. The findings highlighted the importance of DKV screening among physically active females, and the rationale for prescribing individualized exercise intervention to prevent lower limb non-contact injuries.