Objective To use the syphilis helicoid gelatin aggregation experiment (TPPA ) method to compare the results of syphilis serological detection reagents between the ELISA method and the colloidal gold method for evaluating the clinical applica ‐bility of each detection reagent .Methods 6 291 serum samples were selected from the hospitalized patients before surgery or before blood transfusion ,and performed the preliminary screening detection by the ELISA method (reagent A and B) and the colloidal gold method ,the positive or weakly positive specimens in preliminary screening were performed the confirmation test by the TPPA method .Results In 6 291 serum specimens ,the positive cases of the reagent A ,reagent B and colloidal gold method were 66 ,64 and 56 cases respectively ,the positive detection rate of colloidal gold method had no obvious difference between the reagent A and rea ‐gent B (P> 0 .05) .The positive samples were confirmed to be 66 cases by TPPA ,including 61 cases of reagent A and reagent B positive ,and the colloidal gold method had 56 cases .5 cases of reagent A positive and 3 cases of reagent B positive were confirmed to be 3 and 2 cases by TPPA respectively ,the sensitivity of the colloidal gold method had significant difference between the reagent A and reagent B(P< 0 .05) .Conclusion ELISA method has high sensitivity and is easy to realize the automated enzyme‐linked im‐munoassay ,which is suitable for the screening of the patient sample before surgery or before blood transfusion .The colloidal gold method has low sensitivity ,but high specificity ,is simple and convenient which is suitable for large‐scale healthy physical examina‐tion and preliminary screening test before emergency surgery .In order to avoid medical disputes ,the positive samples by the ELISA method and colloidal gold method should be confirmed by the TPPA method .

Chronic inflammation induced by Helicobacter pylori is considered to be one of the main causes of gastric cancer,and CagA is a main virulence factor of H.pylori.The study aimed to investigate the role and mechanism of CagA in host inflammatory response.Mass spectrometry was used to identify the interacting proteins of CagA in AGS cells.By immunoprecipitation and immunofluorescence,the interaction was validated.Pathway expression was detected by immunoblotting after knockdown by using siRNA,and mRNA levels of inflammatory cytokines were detected by quantitative PCR.CagA-induced inflammatory responses were detected in clinical samples using hemoglobin-eosin staining(H&E).Data showed that CagA interacted with SHARPIN.And CagA activated the NF-κB signaling pathway and upregulated the mRNA and protein levels of the inflammatory cytokines IL-6,IL-8,and TNF-α,as compared with the CagA knockout strain(all P<0.05).Knockdown of SHARPIN by siRNA reduced inflammation levels and partially inhibit NF-κB signaling.In clinical samples,CagA-positive samples exhibited stronger inflammatory responses.To sum up,CagA promoted the host inflammatory response,and CagA-induced inflammatory response was reduced when SHARPIN was partially inhibited,suggesting that CagA activates the NF-κB signaling pathway through binding to SHARPIN.

Objective To analyze the risk factors of postoperative joint infection after arthroscopic an-terior cruciate ligament(ACL)reconstruction,so as to provide evidences for prevention.Methods This is a retrospective case control study.Among 20549 arthroscopic anterior cruciate ligament reconstruc-tion performed between year 2002 and 2018,62 was diagnosed as postoperative joint infection,includ-ing 54 males and 8 females,with an average age of 26.5 years.Another 638 without postoperative in-fection was selected using stratified sampling according to the number of operations per year.The gen-eral condition and surgery data of all patients were collected from the electronic medical record sys-tem.Then univariate Logistic regression analysis was conducted to screen risk factors and multivariate analysis was carried out to create a prediction model.Moreover,an artificial neural network(ANN)model was created to predict the probability and compared with Logistic regression model.Results The univariate Logistic regression analysis found 14 factors associated with postoperative joint infection,in-cluding gender,age,height,weight,body mass index(BMI),socioeconomic status,companioned pro-cedure,single or double bundle reconstruction,portal number,tourniquet time,drainage number,pro-phylactic antibiotics,previous knee surgery and companioned illness.The sensitivity,specificity,accura-cy and area under curve of Logistic regression model was 100%,55.6%,60%and 0.843.As for ANN model,the corresponding values were 80%,89.9%,90%and 0.917.Conclusion Risk factors associated with postoperative joint infection include gender,age,BMI,socioeconomic status,surgery date,tourni-quet time,drainage number and previous knee surgery.Both Logistic model and ANN model yield sat-isfying predicting efficacy,with ANN model showing higher accuracy.