BACKGROUND:Chinese herb Cape Jasmine Fruit can activate the chondrocytes and geniposide is an important component in this herb.
OBJECTIVE:To investigate the effect of geniposide on col agen II synthesis in rat chondrocytes cultured in vitro. METHODS:Rat chondrocytes were separated and cultured in vitro. The chondrocytes were then interfered with 25, 50 and 100 mmol/L geniposide. Normal control group was also set. Col agen II mRNA and protein expression was detected with semi-quantitative RT-PCR and western blot analysis, respectively.
RESULTS AND CONCLUSION:RT-PCR and western blot analysis results showed that, geniposide at 25, 50 and 100 mmol/L increased the col agen II mRNA and protein expression (P<0.01). Geniposide can promote the synthesis of col agen II in rat chondrocytes cultured in vitro.

cleaning and disinfection.Results All 28 surveyed medical institutions had separate endoscope disinfection rooms, 89.29% of which had integrated endoscopic cleaning station,17.86% had automatic endoscope washer/disinfector;100% used multi-enzymatic detergent,chose the right disinfectant,monitored disinfectant concentration every day, and implemented standard disinfection time.But only 39.29% changed multi-enzymatic detergent for each endo-scope,cleaning and disinfection personnel in 78.57% of medical institutions wore personal protective equipment correctly.77 digestive endoscopes were detected,the qualified rate was 88.31%.Conclusion Cleaning and disin-fection management of digestive endoscope in secondary and above medical institutions in Suzhou City is generally standardized,there are still some problems in the manipulation procedures,relevant national regulations should be strictly complied with,efficacy of cleaning and disinfection of digestive endoscope should be further improved.

Objective To observe the effect of medical ozone on the expression of IL1R,CXCL-13 and IL24 gene in synovial membrane of osteoarthritis of knee joint. Methods Sixty-five cases of knee osteoarthritis including 11 lost-cases were selected ,and randomly divided into combination group and arthroscopy group with 27 cases in each group. After arthroscopic surgery ,combination group performed intra-articular injection of 40μg/mL concentration of medical ozone 40 mL/week for 2 weeks but arthroscopic surgery group had no ozone injection. Differences of the expression of IL1R I,CXCL13 and IL24 gene and protein in synovium were compared before and after the treatment in two groups by RT-PCR and Western blot. Results Expression of IL1R I gene and protein in synovium of combination group was significantly lower than that of arthroscopy group and it showed statistical significance(P<0.01). Expression of CXCL13 gene and IL24 gene and protein in synovium of combination group was higher than those of arthroscopy group and it had statistical significance (P < 0.05). Conclusions Medical ozone can reduce the symptoms of arthritis and slow synovitis progress through influencing the expression of IL1R, CXCL-13 and IL24 gene. The effect of ozone combined with arthroscopic is better than that of simple arthroscopic debridement,but cannot stop and reverse the progression of the disease completely.

Objective:To evaluate the degree of anxiety and depression of medical staff during the epidemic period of the COVID-19, and to provide theoretical basis for the pertinence psychological intervention timely.Methods:The investigation about the psychological status of 183 medical staffs on duty during the epidemic period was conducted using Self-Rating Anxiety Scale (SAS) and Self-Rating Depression Scale (SDS) from 18th to 20th February, 2020, who came from Tianjin and other provinces. The respondents were divided into first-line and non-first-line groups according to their jobs, and local and non-local groups according to their work places, respectively. The data was collected by "Star Questionnaires" using the mobile terminal, and the statistical analysis was carried out to compare whether there were differences in depression and anxiety among medical staff of different groups.Results:The total SAS score (48.96±9.21) and SDS score (56.69±7.57) of all medical staff were higher than those of the national norm SAS (33.80±5.90) and SDS (41.88±10.57) from 1158 individuals ( P<0.01) . Among 183 medical staff, the anxiety was found in 87 individuals and the rate of anxiety was 47.5%. The depression was found in 134 individuals and its rate was 73.2%. The anxiety combined with depression was found in 69 individuals and its rate was 37.7%. The scores of SAS and SDS of first-line group were (49.40±9.77) and (55.76±6.86) , respectively. And those of non-first-line group were (48.69±8.87) and (57.29±7.96) . They were higher than those of the national norm, and the differences were both statistically significant ( P<0.01) , but there was no difference between the first-line group and non-first-line group. The scores of SAS and SDS of local group were (48.23±9.04) and (56.76±7.05) , while those of non-local group were (50.63±9.45) and (56.54±8.71) , respectively. The differences were statistically significant ( P<0.01) , compared with the national norm respectively. But there was no difference between the local group and non-local group. No matter which classification criteria were used, there was no statistically significant difference in the severity distribution of anxiety and depression among different groups ( P>0.05) . Conclusion:The anxiety and depression exist obviously among the medical staff during the epidemic period of the COVID-19, and the psychological intervention should be taken out in time.

Objective:To evaluate the degree of anxiety and depression of medical staff during the epidemic period of the COVID-19, and to provide theoretical basis for the pertinence psychological intervention timely.Methods:The investigation about the psychological status of 183 medical staffs on duty during the epidemic period was conducted using Self-Rating Anxiety Scale (SAS) and Self-Rating Depression Scale (SDS) from 18th to 20th February, 2020, who came from Tianjin and other provinces. The respondents were divided into first-line and non-first-line groups according to their jobs, and local and non-local groups according to their work places, respectively. The data was collected by "Star Questionnaires" using the mobile terminal, and the statistical analysis was carried out to compare whether there were differences in depression and anxiety among medical staff of different groups.Results:The total SAS score (48.96±9.21) and SDS score (56.69±7.57) of all medical staff were higher than those of the national norm SAS (33.80±5.90) and SDS (41.88±10.57) from 1158 individuals ( P<0.01) . Among 183 medical staff, the anxiety was found in 87 individuals and the rate of anxiety was 47.5%. The depression was found in 134 individuals and its rate was 73.2%. The anxiety combined with depression was found in 69 individuals and its rate was 37.7%. The scores of SAS and SDS of first-line group were (49.40±9.77) and (55.76±6.86) , respectively. And those of non-first-line group were (48.69±8.87) and (57.29±7.96) . They were higher than those of the national norm, and the differences were both statistically significant ( P<0.01) , but there was no difference between the first-line group and non-first-line group. The scores of SAS and SDS of local group were (48.23±9.04) and (56.76±7.05) , while those of non-local group were (50.63±9.45) and (56.54±8.71) , respectively. The differences were statistically significant ( P<0.01) , compared with the national norm respectively. But there was no difference between the local group and non-local group. No matter which classification criteria were used, there was no statistically significant difference in the severity distribution of anxiety and depression among different groups ( P>0.05) . Conclusion:The anxiety and depression exist obviously among the medical staff during the epidemic period of the COVID-19, and the psychological intervention should be taken out in time.