BackgroundIn the present time Photodynamic therapy is a largely experimental treatment modality which is under development for application in both of neoplastic and non–neoplastic diseases. PDT involves a light-sensitive compound (photosensitizer), light and molecular oxygen. The photosensitizer is excited to its singlet state by light of the appropriate wavelength. Responses to photodynamic treatment are dependent on the photosensitizer used the an illumination conditions, the oxygenation status of the tissue and the type of cells involved.CoalTo study new photosensitizers from the pyrazole derivatives which is based on chlorine and porphyrin and produced from sea algae to the lung cancer cell (A549) that is cultured in vitro medium and tested by two ways that are light treatment and dark treatment. Furthermore we define how the photosensitizer dictate cancer cells by test MTT/3-/4.5-dimethylthiazole-2- yl/-2.5-biphenyl tetrzolium bromide a yellow tetrazole/, and how to change the cell morphological characteristics by its micro photo and determine potosensitizers that dictates on the least doze of the cell.Materials and MethodsThe cell line tested was A549 (human lung carcinoma cell). The cell line was obtained from the cell line bank at Seoul National University’s Cancer Research Center (Korea) and were grown in medium RPMI-1640 (Sigma-Aldrich) with 10% fetal bovine serum, glutamine, penicillin and streptomycin at 37 0C in humidified atmosphere of 5% CO2 in air. Phosphate buffered saline (PBS) (Sigma-Aldrich), microscope (Olympus, CK40-32 PH, Japan), Laser irradiation (BioSpec LED 670-700 nm, Russia), ELISA-reader (BioTek, Synergy HT, USA), trypsin-EDTA solution, incubator (37 0C, 5% CO2) were used. The PDT was carried out using a diode laser generator apparatus (BioSpec LED, Russia) equipped with a halogen lamp, a band-pass filter (670-700 nm), and a fiber optics bundle. The wavelength was set at 670±1 nm. Duration of the light irradiation, under PDT treatment, is calculated taking into account the empirically found effective dose of light energy in J/cm2. Figure 1 shows micrographs from an optical microscope illustrating the morphological changes of A549 cells at different points of time after PDT.ResultsThe morphological changes for all tested photosensitizers were revealed by the same patterns, hence we have shown the morphological changes for compound 8 in this paper (Fig 1). Untreated A549 cells as a control did not show any significant morphological changes. The changes in the state and activity of cellular organelles induced by PDT were clearly observed after 3 h. PDT treatment against A549 cells induced plasma membrane disruption and cell shrinkage, indicating the plasma membrane as the main target for the photosensitizer: the membrane of A549 cells began to shrink immediately after PDT, and cell death processes commenced with cytoplasm leakage around the membrane for the first 3 h. After 24 h, the membrane had disintegrated, confirming the loss of cell viability.СоnclusionThe morphological changes were determined in 3 h, 24 h and 48 h after PDT. The bioactivity of 23 new photosensitizers was examined. Among them, 8 photosensitizers showed a promising effect for PDT, therefore, their in vitro biological results are displayed in this study. It was observed from the experimental results that the tested photosensitizers showed more promising effects for PDT or light toxicity. These photosensitizers inhibited more than 50% cells at 2.5 μM after 24 h. Untreated cells as control did not show any significant morphological changes. The PDT treatment cells induced to plasma membrane disruption and cell shrinkage. After 24 h of treatment the membrane was disintegrated, and confirmed the loss of cell viability.

Spent brewer’s yeast Saccharomyces cerevisiae was hydrolysed using bovine pancreatictissue as an enzyme source. The resulting hydrolysate contains 4.7% amino nitrogenand the ratio Namin/Ntot was determined as 0.6. Moreover, other physico-chemicalparameters of the hydrolysate were also comparable with the same products on foreignmarkets. Toxicity of hydrolysate is low (LD50 2,5 g/kg), microbiological and heavy metalcontamination were in required range. The IC50 value of obtained yeast hydrolysate’santioxidative activity according to the DPPH assay was 1.6±0.5 mg/ml, while IC¬50 ofthe yeast hydrolysate rich in Cyclo-His-Pro evaluated as 1.9 mg/mL.

Although all the pharmacy programs (1 public and 2 private) in Mongolia comply with the national standard for pharmacy higher education D 723400 (MNS 5323-126: 2012), competence based learning has not been introduced yet. Also, pharmaceutical public health service needs have not been assessed in community pharmacies of Mongolia. Our goal was to assess the learning needs of pharmacists’ public health competencies and link pharmacy education with the health needs of populations.This cross-sectional, in-pharmacy survey was conducted in a simple random sample of community pharmacies in 6 districts of Ulaanbaatar city central region, which provide price discount on selected drugs through national health insurance. The survey was developed with evidence for reliability and validity and focused on two main needs assessment: pharmaceutical public health (PPH) serviceprovision and PPH competencies learning. Open ended and 3 point scale (1=great benefit; 2=some benefit; 3=no benefit) questions were used to obtain: general information, public health service knowledge, and needs of learning PPH competencies. Descriptive statistics and comparisons using STATA 13 were performed.A total of 248 surveys were obtained (pharmacists n=82; customers n=166). Pharmacist knowledge/skills greatly impacted the public’s decision to use expanded services. The customers reported high levels (75.3%) of needs with pharmacists providing advice on health promotion and healthy life style. 47.6% of the pharmacists replied having knowledge on health promotion and disease prevention is a great benefit to their practice.Our findings indicate that the public is very interested in pharmaceutical public health services and believe they will benefit. Also pharmacists need to be trained ecessary competencies.

Introduction: Due to cervical spondylosispatients with cervical stenosis who hadhyperextension trauma developed spinalcord stress and contusions, tetraplegia anddisability and mortality. Cervical spondylosispatients with hyperextension traumatreatment management still not clearedout throughout the world and very fewresearch has been done in our country.We have been introduced our surgical andrehabilitation comparison research study ofcervical spondylosis trauma in 2015. Our goalis to develop treatment management forthe hyperextension trauma in patients withcervical spondylosis.1. To compare surgical treatmentresults between patients who hadhyperextension injury to the cervical spinewhich were treated at the Departmentof Spinal Surgery of the National Traumaand Orthopaedic Research Center ofMongolia.2. To study biomechanical effects after thesurgeryMaterial and methods: 42 cervicalspondylosis patients with hyperextensioninjury have been chosen retrospectively toevaluate the surgical, conservative, andrehabilitative treatments and results wereanalyzed by SPSS, EXCEL program who weretreated at the Department of Spinal Surgeryof the National Trauma and OrthopaedicResearch Center of Mongolia from 2012-September 2016. Over 20% canal stenosiscases were chosen for laminectomy andanterior discectomy and fusion surgeriesalong with conservative and rehabilitationtreatment. Up to 20% canal stenosis 10cases which is 23.8% of all patients weretreated by conservative treatment.Results: From 42 patients there was 10females and 32 males. Average ages were56±7.9. By the cause of injury 62% werecar accident, 23% motorcycle accident, 10%fall from horse riding and 5% were otherfalls respectively. All patients had neurologicdeficits according to the level of spinal cordinjury. 36 (85.7%) patients had C4-C5 andC5-C6 level injury and 15 (35.7) patients wererecovered from spinal shock after high dosesteroid treatment. According to the X-RAYall patients had cervical spondylosis signand 4 (9.5%) of them had facet fractureswith stable spine condition. After CT scanspinal cord injury located at C3-C4, C4-C5and C5-C6 levels and patients developedfacet arthrosis, ossification of ligaments andintervertebral discs. Clinical signs and CT scans were evaluated and 4 cervical spondylosispatients with hyperextension injury hadlaminectomy and 2 patient had anteriordiscectomy and fusion surgeries. Canalstenosis is decompressed after laminectomysurgery and cervical lordosis is reduced by 7degrees but after anterior discectomy andfusion surgery cervical lordosis is reducedby 3 degrees. Neurological deficit increasinglaminectomy cases had C5 palsy becauseof cord shift (2.4-4mm) which resulted bydenticulate ligament tethering.Conclusion: There were no significantstatistical (P≤.05) differences betweensurgical and rehabilitation treatmentsin cervical spondylosis patients withhyperextension injury who’s canal stenosiswere below 20% [1,2,3,4,5].In our practice itis essential to make laminectomy and anteriordiscectomy and fusion surgeries in 72 hoursafter trauma to help the patient recoverfrom the spinal shock and reabsorption ofcord contusion. For one or two level canalstenosis especially with the OPLL, artificialdisc replacement and ADF surgeries showedbetter results. In multiple level canal stenosiswith OLF and OPLL cases laminectomyand laminoplasty are choice for surgery. Incomparison to foreign study/ Biomechanicalinvestigation of spinal cord injury and diseasein cervical spine Batbayar.K Seoul.2016[2]/ our surgical treatment showed similarresults. Among cervical spondylosis patientswith hyperextension trauma cases whotreated in Spine Department of NationalTrauma and Orthopaedic Hospital, mortalityand disability is increasing and it is essentialto follow correct diagnostic and treatmentalgorithms.

BackgroundTo investigate the pharmacological actions of three groups of drugs containing: Plantago major L. extract, Calendula officinalis L. extract, or mixtures of both. The three investigational drugs were compared to Omeprazole, a standard drug produced in Bulgaria, which inhibits the release of enzymes of the secretary cells of the mucous membranes of the stomach.Materials and MethodsThe effects of the medicinal substances were investigated on “Wistar” breed of white rats. Pathological model of ulcers was formed by injecting the rats with 10% acetic acid (Okabe, Koth.S 1978).The experimental animals were given any of the three versions of the new herbal medicine and compared to the rats given Omeprazole. After treatment the ulcer index, MDA and sialic acids in the serum were determined.ResultThe results showed that in the third day of the investigation, the sialic acid levels in the blood of the test group, which was given the mixture of the two plants, was significantly reduced (p<0.05) compared to the control group.ConclusionThis indicates that medicinal substances given to the rats, which contained the mixture of Plantago major L. and Calendula officinalis L. has accelerated the regeneration of the stomach, decreased the stomach cell damage and overall has antioxidant protective effects.

Background: To investigate the pharmacological actions ofthree groups of drugs containing: Plantago major L. extract, Calendula officinalis L. extract,or mixtures of both. The three investigational drugs were compared to Omeprazole, a standard drug produced in Bulgaria, which inhibits thereleaseof enzymesof the secretary cellsof the mucous membranesof the stomach.[1]Materials and Methods:The effects of the medicinalsubstances were investigated on “Wistar” breed of white rats. Pathological model of ulcers wasformed by injecting the rats with 10% acetic acid./ Okabe, Koth.S1978 /. [2] The experimental animals were given either of the three versions of the new herbal medicine and compared to the rats given Omeprazole. After treatment the ulcer index, MDA and sialic acids in the serum were determined. [3]Result: The results showed that in the third day of the investigation, the sialic acid levels in the blood of the test group, which was given the mixture of the two plants, was significantly reduced (p<0.05) compared to the control group.Conclusion:This indicates that medicinal substances given to the rats, whichcontained the mixture of Plantago major L. and Calendula officinalis L. has accelerated the regeneration of the stomach, decreased the stomach cell damage and overall has antioxidant protective effects.Key words: Stomach, gastric ulcers, acetic acid, sialic acidReferences:1. алтанцэцэг а, амбага м. амьтнû гаралтай зарим бэлдмэлийн ходооднû салст бүрхүүлийн гэмтлээс хамгаалах идэвхи. “монгол туургатнû уламжлалт анагаах ухаан” оу-ûн эрдэм шинжилгээний бага хурлûн илтгэлийн хураангуй уБ .1995, х 40-43.2. Okabe S. Koth J.L and others. “Amer.jour. dig,dis,1971, vol16. pp.277-2893. стальная и.д и др. методопределения мда с помошью тиобарбитуровой кислотû. совр. методû биохимии.м, 1977, с 66-68.

BackgroundThe works done during the research include: conducting photochemical study on the surface of the piece of land on which the medicinal herbs chosen as samples for the research grow; extracting dry infusion from plants; identifying main substance and antibacterial activity of dry infusion; conducting pilot pharmacological experiment with mineral samples.GoalBased on the results of these experiments, we aimed at finding out wound healing functions of Chelidonium majus. L, Stellerae chamaejasme.L etc, widely used in both conventional and modern medicine as well as of such minerals as Sinder, Zeolite, Tormohon, Baragshun after selecting from them.Materials and MethodsAlkaloid, a biologically active substance found in surface of plant land and dry infusion, was determined by titration method, alkaloids such as protopine and berberine by high performance liquid chromatography, coumarin and flavonoid by spectrophotometric method, the amount of infusible substance by scale method. The methodologies of Gatsura.N and Avtandilov.G.G were adopted to make artificial wound on skin and to heal a wound respectively.ResultsAs the research result shows, the surface of the area where Stellerae chamaejasme.L grow, contains 0.33% ±0.004 total coumarin, 0.19 % ±0.002 flavonoid and 37.14 % ±0.61 infusible substance while that of Chelidonium majus.L contains 0.19% ±0.003 total alkaloid, 0.12 % ±0.004 flavonoid, 0.09 % ±0.002 coumarin and 36.27%±0.74 infusible substance. After the infusion was extracted from Stellerae chamaejasme.L and Chelidonium majus.L through method of percolation with mixer, there were 16% and 14.5% dry substance remained in the infusions respectively. These figures were reduced to 12.6% and 11.4% after freezing them in -500C for 72 hours in dry freezing. The fact that total coumarin contained in dry infusion of Stellerae chamaejasme.L is 2.95% and protopine and berberine in that of Chelidonium majus.L, is 28 мг,% and 3.7 мг,% respectively meets the requirements for medicinal plant extracts. It was found during the pharmacological experiment of preparing 25% oil ointment composed of minerals including Sinder, Tormohon, Zeolite, Baragshun that zeolite was the highest effective mineral in healing a wound by wound index. Therefore, we chose zeolite as an ingredient of the wound ointment. Oil ointments of 6 variations with 5% and 10% content were prepared from the dry infusion as well as minerals of the study plants, and pilot pharmacological experiment was conducted on an experimental mouse that had an artificial wound. The result of the experiment proved that oil ointments with 5% content of Stellerae chamaejasme.L, Chelidonium majus.L and zeolite were more effective in healing the wound than others.ConclusionBy photochemical analysis, the chosen plants proved to be meeting the requirements for medicinal plants. Pilot pharmacological experiment showed that zeolite was the one that accelerated a wound healing process more efficiently than others. Oil ointments with 5% content of dry infusion and 5% of zeolite were effective in healing a wound quickly.

BackgroundPreliminary clinical studies indicate that liver extract may be helpful in treating hepatic dysfunction. In addition, liver extract seems to work synergistically with interferon in treating hepatitis C and other viral infections. Laboratory studies indicate that liver extract may have some effects that could be useful in treating certain forms of cancer, such as ability to direct migration of metastasizing cells and inhibition of DNA, RNA and protein formation. More research is needed in these areas to determine liver hydrolysate’s properties.Materials and MethodsSeveral biochemical methods were used for determination of chemical compounds in liver extracts: Total protein and nitrogen content was determined by Kjeldahl method; mineral contents – atomic absorption spectrophotometer; Heme iron content – spectrophotometer; Water soluble vitamins - HPLC method. The pharmacological activities of bovine liver were tested by several pharmacological methods: Acute toxicity – LD50 /Prozorovskii 1978/; Acute hepatitis – Carbon tetrachloride (CCl4) induced liver damage in rats /Skakun et al, 1984/; Biochemical parameters in blood serum – Automatic biochemical analyzer.ResultThe values obtained in determination of the biochemical analysis show that 100 g consumption of studied liver hydrolysate can provide 4.3, 2.1 and 0.3 mg vitamin B1, B3 and B9 respectively. Therefore, present data reveal that liver hydrolysate is a good source of most of the analyzed minerals. The liver hydrolysate contains 56.4% total protein and 4.33% amino nutrient.Conclusions:1. From the results of pharmacological study that involves CCl4 induced acute toxic hepatitis, liver hydrolysate has hepatoprotective effect by protecting the liver cells from injury, improving the regeneration process and by correcting metabolic functions of the liver.2. When tested, hydrolysate’s pharmacological parameters can be analyzed reliably with several liver damage experimental designs, further improvements or the use of new designs such as anemia is needed in further pharmacological study.

BackgroundLiver protecting effect of liver hydrolysate is studied on CCl4 induced by acute toxic hepatitis on rats compared with “Raw Liver Glandular” which is similar product of “Swanson”, USA. Result was confirmed with histological study.Materials and MethodsCarbon tetrachloride-CCl4 is considered as a direct hepatotoxin which produces centri-lobular necrosis and steatosis. The mechanism of acute toxic hepatitis induced by CCl4 involves lipid peroxidation of membrane bound fatty acids which result in destructing the cell membrane and the intracellular organells of the hepatocyte.Result:As study result, after 7 days CCL4 exposure, experimental group’s serum ALAT (p<0.01), GGT (p<0.05) and ALP (p<0.05) levels decreased rapidly compared with control groups.ConclusionAlthough, liver hydrolysate is not medicine for treatment liver damage, it might be improved regeneration process in the damaged liver cells. As result, histomorphological study came to the following conclusion: in the seventh day of the model of acute toxic hepatitis of the liver in rats, cytoplasm vacuoles of hepatocytes were decreased sharply, hepatocytes became multilateral, “Kupffer cells” were increased, young hepatocytes were streamlined as columnar-cells, capillaries were became visible and was began regeneration. There were reported that bovine and pork protein hydrolysate contains essential amino acids with antioxidant effect which prevents liver cells from oxidative stress. Besides it, liver hydrolysate containing amino acids, vitamin B1, B6, folic acid and some minerals improves metabolism and regeneration process of damaged liver cells.

Background: We aimed to study the etiology and transmission route of diseases introducing the modern, rapid and high-sensitivity molecular genetic diagnostic methods for salmonellosis. Material and Method: In the study, we collected 680 stool samples and defined organisms of food intoxication by identification of bacteria, polymerase chain reaction (PCR) and determined serotype and antibiotic resistance. Result: Salmonella spp was detected from the stool of 25 (42.3%) patients out of 59 outpatient clinic and of 170 (27.4%) patients out of 621 inpatient clinic with diagnosis of food intoxication. In total there was detected 195 salmonella spp, and out of this isolated Sal. typhimurum in 193 (98.9%), and Sal. enteritidis was in 2 (1.1%) patients, respectively. We defined Sal. typhimurum in selected 32 cultures and did not detect resistant gene DT-104 ACS-SuT by PCR. Conclusion As resulted in the survey, we defined 195 (28,6) Salmonella typhimurum among the 680 patients who were suffered from food intoxication, and revealed fast foods, animal derived foods such as chicken, fish caused the food intoxication. Sal. typhimurium not resistance to antibiotics.