Objective　To investigate the expression level of serum microrna-493 (mir-493) in patients with acute cerebral infarction (ACI) and its relationship with the prognosis after thrombolysis.Methods　Seventy patients with ACI in our hospital from October 2019 to March 2021 were selected as the study group,and 70 patients with healthy physical examination during the same period were selected as the control group.The serum miR-493 levels of the study group before treatment,3 d,7 d after treatment were detected.Results　The serum miR-493 levels in the study group were higher than that before treatment at 3 days after treatment,however,the serum miR-493 levels in the study group were lower in each time period (P<0.05).Serum miR-493 levels before treatment and neurological deficits in ACI patients Degree,serum VEGF,Ang-2,CRP,and IL-6 levels were negatively correlated (P<0.05).Serum miR-493 levels before treatment,3 d,and 7 d after treatment in the study group with good prognosis were higher than those with poor prognosis patients (P<0.05).The area under the curve (AUC) of serum miR-493 predicting poor prognosis of ACI patients with thrombolytic therapy before treatment,3 d and 7 d after treatment were 0.748,0.851,0.879,respectively,and 7 d after treatment serum miR-493 predicted the largest AUC,and the best prediction sensitivity and specificity were 73.68% and 88.24%,respectively.Conclusion　The expression levels of serum miR-493 in patients with acute cerebral infarction are significantly reduced,and related to the prognosis of patients after thrombolysis,and has certain value in assisting clinical prediction of the prognosis of patients.

Objective　To investigate the relationship of serum astrocyte-derived protein (S100β) and neuron-specific enolase (NSE) in patients with acute cerebral infarction and vascular dementia (VD) and their predictive value.Methods　One hundred and thirty-two patients with acute cerebral infarction (cerebral infarction group) and 60 healthy people who took medical examinations (healthy control group) were collected and levels of serum S100β and NSE were detected by ELISA Patients with cerebral infarction were divided into the non-VD group and VD group according to the Mini Mental State Examination Scale (MMSE) score after 3 months’ treatment Logistic regression was used to analyze the correlation between S100β and NSE and the incidence of VD and ROC curve analysis was used to analyze the early predictive value of S100β and NSE levels for VD.Results　Compared with the healthy control group.the serum levels of S100β and NSE in the cerebral infarction group were significantly increased (P<0.05) and those in the VD group were significantly higher than the non-VD group (P<0.05).Multivariate logistic regression analysis showed that high S100β and high NSE were independent risk factors for VD after cerebral infarction.The sensitivity.specificity and accuracy of S100β and NSE for predicting VD after cerebral infarction were 75.86%,60.19%,63.64% and 68.97%,79.61%,77.27%,respectively.The sensitivity.specificity and accuracy of the combination of the S100β and NSE were 72.41%,90.29%,86.36%,respectively.Conclusion　High levels of S100β and NSE in serum were independent risk factors for VD after cerebral infarction,and the combined diagnosis of S100β and NSE has potential clinical value in predicting the occurrence of VD after cerebral infarction.

Objective To explore the efficiency improvement in segmenting neural network with the application of Transformer + U-Net artificial intelligence (AI) and modeling with the application of Python scripts in three-dimensional (3D) printing brachytherapy. Methods A Transformer + U-Net AI neural network model was constructed, and Adam optimizer was used to ensure rapid gradient descent. Computed tomography or magnetic resonance imaging data of patients were standardized and processed as self-made data sets. The training set was used to train AI and the optimal result weight parameters were saved. The test set was used to evaluate the AI ability. Python programming language was used to write an automated script to obtain the output segmentation image and convert it to the STL file for import. The source applicator and needle could be automatically modeled. The time of automatic segmentation and modeling and the time of manual segmentation and modeling were entered by two people, and the difference was verified by paired t-test. Results Dice similarity coefficient (DSC), mean intersection over union (MIOU), and Hausdorff distance (HD95) were used for evaluation. DSC was 0.9341, MIOU was 0.8762, and mean HD95 was 2.516. The mean time of manual segmentation was 1187 s, and the mean time of AI segmentation was 145 s (P < 0.01). The mean time of manual modeling was 321 s, and the mean time of modeling with Python automated scripts was 18 s (P < 0.01). Conclusion The application of Transformer + U-Net automatic segmentation and Python automated scripts can effectively improve work efficiency in the 3D printing brachytherapy for cervical cancer.

【Objective】 To analyze the infection status of syphilis among voluntary blood donors in Nanyang area, aimed to provide references for the recruitment of blood donors and ensure blood safety. 【Methods】 Enzyme-linked immunosorbend test(ELISA method) was used to screen the blood samples of 45 6981 voluntary blood donors from 2013 to December 2020, and the reactive blood samples of TP antibody were confirmed by treponema pallidum particle agglutinaation(TPPA method), the syphilis infection status in different population was compared and analyzed. 【Results】 Among 456 981 blood samples, 1 088 were reactive for anti-TP by ELISA method, and 714 were positive for syphilis(714/456 981, 0.15%); the total positive rate and positive rate of male and female in voluntary blood donors decreased year by year; female donors(347/180 987, 0.192%), first-time donors(613/324 188, 0.189%), whole blood donors(708/433 432, 0.163%) and married donors(668/399 899, 0.167%) had a significantly higher syphilis seroprevalence than male donors(367/275 994, 0.133%), repeated donors(101/132 793, 0.076%), apheresis platelet donors(6/23 549, 0.025%) and unmarried donors(46/57 082, 0.080%), respectively, and the differences between two groups were statistically significant (P<0.05); the positive rate in donors with junior high school and below was significantly higher than other groups; the positive rate in donors of 35~44 years old was higher than other age groups, with statistically significant difference(P<0.05); syphilis seroprevalence among different occupational groups was statistically significant (P<0.05); Among 714 donors, co-infection with other viruses were noted in 40 cases(40/714, 5.60%), and only TP infection in 674 cases(674/714, 94.40%). 【Conclusion】 The TP seroprevalence among voluntary blood donors in Nanyang area showed a downward trend from 2013 to 2020, the majority of TP seropositive population are first-time donors, married donors, whole blood donors, donors with lower academic qualifications, and farmers/individual donors. Strengthening the consultation towards high-risk blood donors before blood donation, and recruitment of blood donors from low-risk groups can reduce / avoid the transmission of syphilis through blood transfusion and ensure the safety of blood transfusion.

Animals ; Liver Cirrhosis/drug therapy* ; Non-alcoholic Fatty Liver Disease/drug therapy* ; Rats ; Rats, Sprague-Dawley ; Sesquiterpenes/pharmacology* ; Signal Transduction

OBJECTIVE To explore the improving effect of luteolin （Lut） on placental dysfunction in rats with gestational diabetes mellitus （GDM） and its potential mechanism based on hedgehog （Hh） signaling pathway. METHODS Twenty female rats were randomly selected as a control group and fed a normal diet. The remaining female rats were fed a high-fat and high-sugar diet for 8 weeks and then caged with male rats. Pregnant rats were administered 35 mg/kg streptozotocin intraperitoneally to establish GDM models. Successfully modeled female rats were randomly allocated to model group， SAG group （Hh signaling pathway activator SAG 50 mg/kg）， Lut low-dose group （Lut 40 mg/kg）， Lut high-dose group （Lut 80 mg/kg）， and Lut high+ITR group （Lut 80 mg/kg+Hh signaling pathway antagonist itraconazole 50 mg/kg）， with 20 rats in each group. Female rats in each drug group were intubated with the corresponding drug solution once a day for 19 days. After the final administration， the serum glucose- fat metabolic parameters （levels of fasting blood glucose and fasting insulin， insulin resistance index）， placental quality， placental permeability [Evan’s blue （EB） content]， and pathological changes in placental tissue were observed. The activities of superoxide dismutase （SOD）， the contents of malondialdehyde （MDA） and reduced glutathione （GSH）， and the protein expressions of Sonic Hh （Shh）， Patched-1 （Ptch1）， Smoothened （Smo） and Gli family zinc finger-1 （Gli1） in placental tissue were detected. HBB_ RESULTS Compared with the control group， rats in the model group showed narrow capillary lumens， perivascular fibrosis in placental tissue， and a significant increase in serum glucose-fat metabolic parameters， placental quality， contents of EB and MDA， while there was a significant decrease in SOD activity， GSH content， and protein expressions of Shh， Ptch1， Smo and Gli1 （P＜0.05）. Compared with the model group， rats in the SAG group， Lut low-dose and high-dose groups had widened capillary lumens， a significant decrease in perivascular fibrosis in placental tissue， serum glucose-fat metabolic parameters， placental qualities， EB and MDA contents， while there was a significant increase in SOD activities， GSH contents， and protein expressions of Shh， Ptch1， Smo and Gli1 （P＜0.05）， with the high-dose group showing no significant difference compared to the SAG group （P＞0.05）. The Hh signaling pathway antagonist itraconazole could significantly reverse the improving effects of Lut on the above indicators （P＜0.05）. CONCLUSIONS Lut can improve glucose metabolism parameters of GDM rats， reduce placental permeability， alleviate pathological damage to placental tissue， and reduce oxidative stress. These effects may be related to the activation of the Hh signaling pathway.

Psoriasis vulgaris is notoriously difficult to treat and prone to recurrence. Traditional Chinese medicine （TCM）， however， has shown considerable efficacy in mitigating or suppressing such recurrence. The underlying reason lies in the TCM concept of "latent pathogens"， which are prone to be reactivated by external pathogenic factors， thereby triggering relapse. At the early stage of recurrence， manifestations of "latent fire" often appear externally. If treatment is not thorough， the condition may shift into a state of "stalemate between healthy Qi and pathogenic factors"， in which the disease appears on the skin but is rooted in deeper pathological layers， remaining unresolved and accumulating internally. Over time， blood stasis arises from fire， and the fire further congeals due to stasis， leading ultimately to recurrent flare-ups. This aligns with the modern immunological concept of "immunological memory" mediated by tissue-resident memory T cells （T_RM） in the skin， which corroborates the TCM view of "latent fire inducing blood stasis". The interaction between T_RM and keratinocytes （KC） parallels the entanglement of latent fire and latent stasis， both of which are deeply entrenched and difficult to resolve. The core pathogenesis of recurrent psoriasis vulgaris lies in "latent fire causing blood stasis". The hallmark is the deep concealment and persistence of latent fire and stasis， which linger and await an opportunity to reemerge. Based on this understanding， Xijiao Dihuangtang is employed to cool the blood， resolve stasis， and eliminate latent pathogens， and treatment is tailored according to the disease stage through three-phase syndrome differentiation. In the progressive stage， both exterior and interior are treated， with emphasis on clearing latent fire. In the stationary stage， the focus shifts to dispelling latent stasis and simultaneously regulating the Zang-fu organs. In the regressive stage， efforts are made to prevent the retention of latent pathogens and to strengthen healthy Qi. Accordingly， drugs effective in dispersing wind and clearing heat， pungent-moistening and dredging the collaterals， and tonifying deficiency and moistening dryness are often employed to achieve optimal outcomes. The precise mechanisms by which Xijiao Dihuangtang treats recurrent psoriasis vulgaris remain to be fully elucidated. Current research suggests it may intervene in the recurrence process through inhibiting KC proliferation via the PI3K/Akt/mTOR signaling pathway and glycolysis， regulating the Th1/Th2 and Th17/Treg cell balances to restore immune homeostasis， suppressing inflammatory cytokine production to alleviate the inflammatory response， modulating angiogenesis-related factors， such as vascular endothelial growth factor A （VEGF-A） and matrix metalloproteinase-9 （MMP-9）， to control disease progression， and restructuring the gut microbiota to modulate systemic immunity and thereby influence the course of disease recurrence.

Objective To investigate the malaria vectors in Nanyang City. Methods Two townships were selected as malaria vector surveillance sites in Nanyang City from 2005 to 2017, including Mazhenfu Township in Tanghe County and Huaiyuan Township in Tongbai County, and the malaria vectors were investigated using the semi-overnight human baiting trapping method and mosquito trapping in nets. Results The mean density of Anopheles sinensis was 0.65 to 22.30 mosquitos/(person · hour) in the two surveillance sites in Nanyang City using the human baiting trapping method from 2005 to 2017, and the peak density was detected in June and July. An. anthropophagus was captured only in Tongbai County during the period between 2008 and 2011, with a mean density of 0.03 to 1.10 mosquitos/(person · hour). The mean density of An. sinensis was 0.08 to 0.24 mosquitos/(net · night) using the mosquito trapping in nets from 2005 to 2011, and the peak density was detected in July and August, while few An. anthropophagus captured only in Tongbai County, with a mean density of 0.01 to 0.02 mosquitos/(net · night). Conclusions There are malaria vectors in Nanyang City, including An. sinensis and An. anthropophagus, and An. sinensis is the dominant mosquito species.

OBJECTIVE： To establish a method for amygdalin， paeoniforin， baicalin， glycyrrhizic acid， emodin and chrysophanol in Dahuang zhechong pills. METHODS： HPLC method was adopted. The determination was performed on Welchrom C18 with mobile phase consisted of methanol-0.1％ phosphoric acid （gradient elution） at the flow rate of 1.0 mL/min. The column temperature was set at 30 ℃， and the detection wavelengths were set at 210 nm （amygdalin）， 230 nm （paeoniforin， baicalin） and 250 nm [glycyrrhizic acid （calculated with ammonium glycyrrhizinate）， emodin， chrysophanol]. The sample size was 10 μL. RESULTS： The linear ranges of amygdalin，paeonifiorin，baicalin，ammonium glycyrrhizinate， emodin and chrysophanol fell within the ranges of 21.028-157.71，2.052-90.390， 34.288-257.16， 8.252 0-61.890， 3.272 0-24.540，4.768 0-35.760 μg/mL （all r≥0.999 2）. The limits of detection were 0.105 0， 0.121 0， 0.068 6， 0.082 5， 0.024 6， 0.017 9 μg/mL. The limits of quantitation were     0.263 0， 0.362 0， 0.171 0， 0.268 0， 0.065 5， 0.047 7 μg/mL. RSDs of precision， stability（12 h）and reproducibility tests were lower than 2.00％ （n＝6）. The  average  recoveries were 96.01％-100.76％ （RSD＝1.63％，n＝6）， 97.09％-101.86％ （RSD＝1.50％，n＝6）， 99.70％-101.99％（RSD＝0.82％，n＝6），96.29％-99.52％（RSD＝1.35％，n＝6）， 98.47％-102.14％ （RSD＝1.35％， n＝6） and 97.19％-99.16％（RSD＝0.69％，n＝6），respectively. CONCLUSIONS： The method is accurate， simple and reproducible for simultaneous determination of amygdalin， paeoniforin， baicalin， glycyrrhizic acid， emodin and chrysophanol in Dahuang zhechong pills.

Objective: To investigate the immune enhancement effect of red ginseng aqueous extract in mice. Methods: The aqueous extract of red ginseng was obtained by water extraction and concentration, and the aqueous extract 10 ml was equivalent to raw material 2.86 g. The experiment was divided into the blank control group, low dose group of red ginseng aqueous extract 0.24 g/(kg•d), middle dose group 0.48 g/(kg•d) and high dose group 1.43 g/(kg•d). In order to explore the immunoregulation effect of red ginseng aqueous extract, Organ/body weight ratio measurement, the delayed type hypersensitivity (DTH) reaction, the spleen lymphocyte transformation experiment induced by Con A, the detection of antibody-producing, the determination of serum hemolysin experiments, the clearance rate of carbon particles, the phagocytosis effect of macrophages with chicken red blood cell experiments, and the determination of natural killer cell activity were carried out with the mice. Results: Compared to the blank control group, the tow edema degree (0.62 ± 0.14 mm, 0.53 ± 0.12 mm vs. 0.36 ± 0.10 mm) of low dose group and medium dose group significantly increased (P<0.05). The ability of proliferation of T lymphocytes (0.173 ± 0.054, 0.189 ± 0.063 vs. 0.098 ± 0.012) of low dose group and high dose group significantly increased (P<0.05). The number of haemolytic empty spots (137.49×10³ ± 24.73×10³, 148.43×10³ ± 27.53×10³ vs. 112.96×10³ ± 26.28×10³) of medium dose group and high dose group significantly increased (P<0.05). The phagocytosis rate (35.67% ± 3.82%, 49.26% ± 6.54%, 57.92% ± 7.36% vs. 24.34% ± 4.22%) and index (0.72 ± 0.23, 0.82 ± 0.15, 0.91 ± 0.26 vs. 0.35 ± 0.11) of low-, medium-, high-dose group significantly increased (P<0.05). However, there was no statistical difference in the organ/body weight ratio of the experimental animals, the determination of serum hemolysin experiments, the clearance rate of carbon particles, and NK cells activity. Conclusion Red ginseng aqueous extract has an exact role in immunity improvement in mice.