1.Prevention and Treatment of Bile Duct Injury in Laparoscopic Cholecystectomy
Guangdong PAN ; Nansheng CHENG ;
Chinese Journal of Bases and Clinics in General Surgery 2003;0(03):-
Objective To investigate the prevention and treatment strategy of bile duct injury (BDI) in laparoscopic cholecystectomy (LC). Methods Latest progress was reviewed based on recent documents and the experience on BDI in LC in our department. Results With the popularity of LC, BDI in LC is increasing. The reasons include illegibility and variability of local anatomy in gallbladder trigone,injury caused by galvanothermy, as well as operator’s over confidence. In order to prevent BDI, we should apply more blunt dissection, not to use electrocogulation if possible and to study local anatomy and its variance clearly. The common bile duct and common hepatic duct should be clearly identified. Intraoperative cholangiography, laparoscopic ultrasonography and hepatobiliary scintigraphy are selections as necessary.The treatment of BDI depends on the type of BDI and its site and local condition.The treatment includes end to end anastomosis, repairing the defect, choledochoduodenostomy, Roux en Y choledochojejunostomy and so on. T tube should be maintained in place for more than half a year after operation.Conclusion The key to improve the prognosis of BDI is prevention and treatment in proper time and in correct way.
2.Experimental Study on PPAR? Ligands Regulating the Inflammatory Cytokines in Human Gallbladder Epithelial Cells
Jiangwen LIU ; Nansheng CHENG ; Guangdong PAN ; Xianze XIONG ; Lan YAN
Chinese Journal of Bases and Clinics in General Surgery 2003;0(04):-
Objective To study the regulatory ability of peroxisome proliferator-activated receptor ?(PPAR?) ligands to the inflammatory response in human gallbladder epithelial cells. Methods Culture human gallbladder epithelial cells and identify them . Cells were treated for 24 hours with 0, 10 ?mol/L, 20 ?mol/L, 30 ?mol/L, 50 ?mol/L and 100 ?mol/L of Ciglitazone during cellular growth peak(5th day), then stimulated them with hIL-1? 5 ng/ml for 2 hours and measured the concentration of IL-6、IL-8 and TNF-? in cellular supernatants by riadioimmunoassay. Results Contrasted with control group, the expression of IL-6 and IL-8 in each test group were inhibited ((P
3.STUDIES ON THE ANTICARCINOGENIC EFFECTS OF POLYSACCHARIDE FROM LAO SHAN POLYSTICTUS VERSICOLOR
Yusen YAN ; Jinsheng WANG ; Minghu CHEN ; Nansheng PAN
Medical Journal of Chinese People's Liberation Army 1982;0(03):-
Polysaccharide from Lao Shan Polystictus versicolor possesses a significant inhibitory effect on transplanted S180 in ICR mice, and the inhibition rate after intraperitoneal injection of 10 mg/kg of the drug was 60-70%. Oral administration in certain dosage could also inhibit tumor growth.Polysaccharide was given intraperitoneally one week before the transplantation of ascitic sarcoma S180 It showed that the polysaccharide could prevent the growth of ascitic S180 effectively and prolong the life span of the mice. Peritoneal injection of the polysaccharide could enhance the capacity of macrophages to release H2O2 and of the spleenic lymphocytes to take 3H-TdR. It has been demonstrated that polysaccharied from Lao Shan Polystictus versicolor was not cytotoxic, and its anticarcinogenic effect was host mediated.