MicroRNAs (miRNAs) are a class of highly conserved single-stranded RNA molecules that modulate gene translation. By targeting the mRNA of protein- coding genes, miRNAs play a critical role in neuronal differentiation, cell proliferation, apoptosis and metabolism. There are a lot of miRNAs in central nervous system, which are not only closely linked to development, differentiation and function of nerve cells, also play an important role in nerve lesions and dysfunction after cerebral ischemia. Specific miRNA through their own changes affect their target gene expression levels after focal cerebral ischemia, involving in the protection against apoptosis in neurons and regeneration after cerebral ischemia. A full understanding of the specific microRNA function and its underlying mechanism in the brain can provide a new strategy for the prevention, diagnosis, and treatment of ischemic cerebral injury at gene level.
Animals ; Brain Ischemia ; Humans ; MicroRNAs

Reflecting on the historical responsibility of Chinese medical ethics at present， it is necessary to place oneself in the grand context of promoting the process of building a community with a shared future for mankind. Safety， development， and civilization constitute the three important ethical dimensions of a community with a shared future for mankind， which are not only ethical orientations， but also ethical appeals. These provide an important direction for Chinese medical ethics to deeply consider its role in the process of a community with a shared future for mankind. The historical responsibilities of Chinese medical ethics in the process of promoting a community with a shared future for mankind mainly include： confronting moral and interest issues， adhering to the patient-centered approach， and ensuring patients’ life safety； playing the role of ethical regulation， achieving the practical effects of medical ethics， and maintaining social security and stability； implementing the responsibility of medical ethics education， shaping medical professionalism， and promoting the development of healthy China； inheriting the excellent traditional culture， pursuing medical excellence and justice， and promoting the development of social morality； pursuing common health values， building a community with a shared future between doctors and patients， and promoting the progress of human. A global community of health for all provides the most direct practical platform for Chinese medical ethics to fulfill its historical responsibility in the process of building a community with a shared future for mankind. It directly promotes Chinese medical ethics to the world， and Chinese ethics facing the world will play an increasingly important influence in self-renewal.

OBJECTIVE: To investigate factors that affected the distribution of rehydrated particle size of freeze-dried docetaxel liposome and develop a technique of producing docetaxel liposome that can meet clinical requirements. METHODS: The single factor method was utilized in this study. Docetaxel liposome were prepared using different types of negatively charged phospholipids, or lecithins with varying phosphatidylcholine (PC) content, or various cryoprotectants at different gradient concentrations, followed by freeze-drying with different protocols. The particle sizes of corresponding rehydrated products were compared and the effects of different factors were analyzed. RESULTS: The optimal liposomal membrane was composed of dipalmitoyl phosphatidic acid (DPPA), 20% trehalose and lecithin containing 92% PC. The best freeze-drying protocol included following steps; flash freezing, 19 h of sublimation-drying and 9 h of desorption-drying. CONCLUSION: The parameters, including negatively charged phospholipids, cryoprotectants, PC content of lecithin and lyophilization protocols, had various effects on the particle sizes of reconstituted docetaxel liposome.

To study the effect of glucagon-likepeptide 1(GLP-1)receptor agonist on insulin resistance and hepatic oxidative stress in rats with diabetes mellitus combined with nonalcoholic fatty liver disease. 36 male SD rats were served as the experimental animal and randomly divided into control group, model group, and GLP-1 group. The rats of control group were given routine diet with intraperitoneal injection of normal saline, those in model group were given high fat diet and intraperitoneal injection of normal saline, while GLP-1 group rats were fed with high fat diet and intraperitoneal injection of liraglutide. After 4 weeks of treatment, insulin resistance, lipid metabolism, liver injury and oxidative stress were all assessed. Serum fasting blood glucose, fasting insulin, total cholesterol, triglyceride, alanine transaminase(ALT), aspartate transaminase(AST)levels and total cholesterol, triglyceride contents in liver tissue, and as well as homeostasis model assessment for insulin resistance(HOMA-IR)levels of model group were significantly higher than those of control group, complex insulin sensitivity index(ISIcomp)level was significantly lower than that of control group; serum fasting blood glucose, fasting insulin, total cholesterol, triglyceride, ALT, AST contents and HOMA-IR levels of GLP-1 group were significantly lower than those of model group, ISIcomp level was significantly higher than that of model group; superoxide dismutase(SOD), glutathione peroxidase(GSH-Px), catalase(CAT)contents in liver tissue of model group were significantly lower, while malondialdehyde content and SOD, GSH-Px, CAT, NF-E2 related factor-2(Nrf-2), antioxidant response element(ARE), heme oxygenase-1(HO-1), quinone oxidoreductase-1(NQO-1), glutathione thiol transferase(GST)mRNA expression were significantly higher than control group; SOD, GSH-Px, CAT contents and SOD, GSH-Px, CAT, Nrf-2, ARE, HO-1, NQO-1, GST mRNA expression in the liver tissue of GLP-1 group were significantly higher, while malondialdehyde content was significantly lower than that of model group. GLP-1 receptor agonist reduces insulin resistance and liver oxidative stress injury in diabetic rats with nonalcoholic liver disease.

After the articular cartilage injury, the metabolic level is increased during the progressive degeneration, the chondrocytes secrete a variety of inflammatory factors, and the original cell phenotype is gradually changed. For a long time, a large number of researchers have done a lot of researches to promote anabolism of chondrocytes and to maintain the stability of chondrocyte phenotype. There are many molecular signaling pathways involved in the process of promoting cartilage repair. This review focuses on the key signaling molecules in articular cartilage repair, such as transforming growth factor-beta and bone morphogenetic protein, and reveals their roles in the process of cartilage injury and repair, so that researchers in related fields can understand the molecular mechanism of cartilage injury and repair widely and deeply. Based on this, they may find promising targets and biological methods for the treatment of cartilage injury.
Bone Morphogenetic Proteins ; physiology ; Cartilage, Articular ; growth & development ; injuries ; Chondrocytes ; physiology ; Humans ; Regeneration ; Signal Transduction ; Transforming Growth Factor beta ; physiology

OBJECTIVETo explore the treatment of casualties who suffered from multiple injuries in earthquake and had been treated by damage control (DC) immediately,and the definite postponed surgery time by way of APACHE II score.

METHODSFrom May to June 2008, 21 casualties who had undergone DC accepted further treatment of medicine and surgery, and recovered physical potentiality in the intensive care unit (ICU). APACHE II score was adopted to evaluate the casualties' physical potentiality. Then, internal fixation of intra-articular fractures and centrum fractures, alternation from external fixation to internal fixation, reconstruction of soft tissue was applied by stages according to the plan.

RESULTSAll the patients were effectively cured after definite postponed surgery. There was less complication, low deformity ratio and no death happened.

CONCLUSIONTimely evaluation of the casualties' physical potentiality, appropriate surgery time and exact surgery can reduce the casualties' complication, deformity rate and death rate.

Adolescent ; Adult ; Aged ; Aged, 80 and over ; China ; Earthquakes ; Female ; Fracture Fixation ; Fractures, Bone ; complications ; surgery ; Humans ; Intensive Care Units ; Male ; Middle Aged ; Multiple Trauma ; complications ; surgery ; Treatment Outcome

Integration of Chinese medicine and Western medicine is the necessary outcome in the reality of co-existence of the two medicines nowadays, also an inevitable tendency of cross-comprehensive, systematization, internationalization and diversification in the progress of science development and research. Experienced over half a century's self-innovation, lots of achievements in clinical and experimental studies have been achieved by the integrated Chinese and Western medicine (ICWM), but it still remains in the primary period of developing, and some deficiencies existed yet. So, we should have the foresight and mind of "Harmony in Diversity" and make effort to establish a series of independent and perfect theoretical system, for making more breakthrough development of ICWM.
Clinical Medicine ; Integrative Medicine ; methods ; Medicine, Chinese Traditional ; methods

OBJECTIVETo study the effects of soil factors on the active components in Radix Ophiopogonis.

METHODThe content of polysaccharide, flavonoids, saponins, water-soluble extract and inorganic elements in Radix Ophiopogonis gathered from 7 different places were compared, physical and chemical properties and inorganic elements of soil were analyzed. The path and grey connection analysis were applied for studying the effects on the active components of Radix Ophiopogonis.

RESULT AND CONCLUSIONThe concentrations of mineral elements in plant were mainly adjusted by active absorption. The active components in Radix Ophiopogonis was primarily effected by soil enzyme activity, the other important factors were potassium, pH, and organic matter. K, Fe, Mn, B, Ba, Zn of soil also had much influence on it then others inorganic elements.

Flavonoids ; analysis ; Ophiopogon ; chemistry ; Plant Extracts ; analysis ; Polysaccharides ; analysis ; Soil ; analysis

This paper provides introduction and analysis of common malfunctions of ventilators System from the aspect of an engineer in the hospital, with a focus on feasible solutions to malfunctions.
Equipment Failure ; Maintenance ; Ventilators, Mechanical

OBJECTIVETo study the quality of medicinal material of Thesium chinense.

METHODThe active ingredients of total flavonoids, kaempferol, mannitol, polysaccharides were selected as the evaluative indicators to comprehensively evaluate the quality of medicinal material of T. chinense.

RESULTThe total flavone 3.38%, kaempferol 0.984 7 mg x g(-1), mannitol 6.12%, polysaccharides 14.08% and water soluble extract 30.28% were measured in the sample of T. chinense. There was a certain correlation among these selected active ingredients. The compositions of most biological activities component in T. chinense of different populations were similar, but the contents were different significantly. The content of activities component in T. chinense showed a general tendency of declining during growth period and with the increase time of storage.

CONCLUSIONFlavone, kaempferol, mannitol, polysaccharides should be selected as the indicators to evaluate the quality of medicinal material of T. chinense.

Flavones ; analysis ; Mannitol ; analysis ; Materia Medica ; chemistry ; Polysaccharides ; antagonists & inhibitors ; Santalaceae ; chemistry