Moral emotion education of medical students should begin with arousing human nature of students and making them to have the sense of consciousness to patients, which takes sympathy as the core. However, when faced with the increase of doctor-patient contradiction, students are easy to suspect the general sense. So students should be guided to understand the necessity of moral emotion in professional activity of doctors, and thus to adhere to treat patients with emotion due to responsibility. On this basis, through combining the education of having emo-tion to patient with working reasonably, medical students will gradually take treating patients with emotion as their own compulsory emotion, and to realize the transformation from human nature to medicine nature.

Clinical research initiated by researchers is one of the important means to promote new understanding of drugs and treatment methods.At this stage,the clinical researches initiated by the researchers frequently appear all kinds of irregularities.In order to solve out this problem,the paper points out suggestions about strengtheningself-discipline of researchers,self-regulation of conscience,and self-responsibility of carefulness.Meanwhile,it is also important to abide by the relevant ethical codes and consciously accept the third party supervision of IRB.Joint efforts should be made to protect safety and rights of human subjects.

No abstract available.
Breast* ; Osteosarcoma*

OBJECTIVEThis study aimed to investigate the effects of hirudin on the expression of transforming growth factor (TGF-β1) and basic fibroblast growth factor (bFGF) in human gingival fibroblasts (HGFs) in vitro, as well to explore its func- tion in the mechanism of gingival remodeling.

METHODSAfter culturing was performed with classic tissue-explant method, HGFs were derived from normal gingival and gingival hyperplasia tissues followed by orthodontic treatments with different concentrations of hirudin. The mRNA and protein expression levels of TGF-β1 and bFGF were respectively detected by real time quantity polymerase chain reaction and immunocytochemistry.

RESULTSCompared with normal HGFs, TGF-β1 expression promoted collagen synthesis of fibroblasts, whereas bFGF collagen synthesis was decreased in hyperplasia HGFs without hirudin (P < 0.05). Hirudin significantly upregulated the expression levels of bFGF but downregulated TGF-β1 in hyperplasia HGFs (P < 0.05).

CONCLUSIONOrthodontic force may influence the balance of collagen synthesis and degradation in HGFs. Hirudin may modulate the balance of HGF collagen metabolism, thereby promoting gingival remodeling.

To explore the relationship and possible mechanism of reproductive function of bisphenol A (BPA) with polycystic ovary syndrome (PCOS).Serum specimens were collected from 43 PCOS patients and 37 healthy women.And the level of BPA was detected by enzyme-linked immunosorbent assay (ELISA).The serum level of BPA in PCOS were significantly higher than that in the control group.And the inter-group difference was significant (P ＜ 0.05).The patient level of BPA was positively related with obesity and basic luteinizing hormone (LH) (P ＜ 0.05).In the PCOS group,no significant correlation existed between hyperandrogenemia and non-hyperandrogenemia (P ＞ 0.05).Although no difference existed between insulin and non-insulin resistance,the level of BPA in insulin resistance group was lower than in non-insulin resistance group.A higher level of BPA may be associated with obesity and basic LH in PCOS.

Objective To evaluate the efficacy and safety of HLA identical and haploidentical related allogeneic hematopoietic stem cell trans?plantation in the treatment of acute myeloid leukemia(AML)and myelodysplastic syndrome(MDS). Methods A total of 21 patients with AML and 8 patients with MDS who underwent allogeneic hematopoietic stem cell transplantation in our hospital from April 2011 to April 2016 were ana?lyzed retrospectively,including 16 cases of HLA?identical allogeneic HSCT,10 cases of haploidentical allogeneic HSCT,and 3 cases of syngeneic HSCT. BUCY2 or TBI plus CY ± chemotherapeutic agents was the regular conditioning regimen. No graft versus host disease(GVHD)prophylax?is was required for syngeneic HSCT,but cyclosporine in combination with methotraxate was essential for allogeneic HSCT,cyclosporine,methotrax?ate,antithymocyte globulin,mycophenolate mofetil and glucosteroids for haploidentical HSCT. Results All patients achieved fully donor?originat?ed hematopoiesis. Two patients died of severe acute GVHD within 100 days post HSCT. Acute GVHD with gradeⅡ?Ⅳoccurred in 23.1%(6/26) patients,chronic GVHD in 50%patients,therapy and relapse?relevant mortality was 4/29(13.8%)and 6/29(20.7%)cases within a median follow?up of 23(1?60)months,respectively. Two?year overall survival and leukemia free survival rates are 68.09%( 95%CI:45.77%?82.78%)and 60.22%(95%CI:38.19%?76.55%),respectively. High risk AML is still the main challenge to long?term leukemia free survival. Conclusion HLA identical and haploidentical allogeneic HSCT for AML and MDS is safe ,effective and feasible. Minimal residual disease monitoring and pre?ventative as well as preemptive intervention is necessary for improving prognosis of high risk AML.

OBJECTIVE: To develop the single-tube one-step methylation variable position (MVP) analysis technology-single-tube post-digestion PCR-melting curve analysis (PDP-MCA). METHODS: Based on differentially methylated region (DMR) reported previously as the model, a set of primers with different melting temperatures of products in the two sides of MVP were designed. By using the FastDigest methylation-sensitive restriction enzyme (MSRE), DNA digestion, multiplex amplification, MCA detection and MCA profiles were performed in a single reaction tube. Same samples (peripheral venous blood, semen, and vaginal fluid, 5 samples each type) were tested by single-tube one step MVP and traditional MSRE-PCR MCA technology. To verify the feasibility of this method, the results were compared with that of the traditional technology. The MCA/HRM profiles of different samples were analyzed and compared. RESULTS: When the melting temperature of the fragments had a differential of 2 degrees C, the MCA melting peaks separated well, and MCA detection after multiplex amplification was successful. The single-tube PDP-MCA assay was developed, which integrated multiple reactions (digestion, amplification and detection) into one tube. By this method, the sample-specific profiles and data were analyzed in 2 h, which is similar to that of the traditional method. The rapid classifications of the samples were also realized. CONCLUSION Multiplex MVPs can be analyzed in a single closed-tube. The single-tube PDP-MCA technology is a simple, fast, and automatable method. It can be used for detection of DNA methylation variations.
DNA/isolation & purification* ; DNA Methylation/genetics* ; DNA Primers/genetics* ; Humans ; Multiplex Polymerase Chain Reaction/standards* ; Nucleic Acid Denaturation

Forensic entomotoxicology is a branch of forensic medicine, which applies entomology, toxicology and other related studies to solve the poisoning cases. It has an obvious advantage in the investigation on poisoning death. Based on the expounding definition and research of entomotoxicology, this paper reviews research progress and application value in some aspects of forensic medicine, such as the effects of drugs/toxins on the growth and development of sarcosaphagous insects and the qualitative and quantitative analysis of the drugs/toxins in the poisoned body tissue.
Animals ; Death ; Entomology/methods* ; Forensic Medicine/methods* ; Humans ; Insecta ; Postmortem Changes

The combination of the various diagnostic methods of palpable breast mass has improved the diagnosticaccuracy. From Sep.1983 to Aug. 1985 pathologically proven 85 patients with palpable breast masses examined withX-ray mammography, ultrasonography, penumomammography and aspiration cytology at Korea Cancer Center Hospital wereanalyzed. The diagnostic accuracies of each methods were 77.6% of mammogram, 74.1% of ultrasonomgram, 90.5% ofpenumommaogram and 92.4% of aspiration cytology. Pneumommamograms was accomplished without difficulty orcomplication and depicted more clearly delineated mass with various pathognomonic findings; air-ductal pattern infibroadenoma(90.4%) and cystosarcoma phylloides(100%), air-halo in fibrocystic disease(14.2%), fibroadenoma(100%),cystosarcoma phylloides (100%), air-cystogram in cystic type of fibrocystic disease(100%) and vaculoar pattern orirregular air collection without retained peripheral gas in carcinoma.
Breast ; Humans ; Korea ; Mammography ; Ultrasonography

Objective To explore clinical effect of Dermatix Ultra on repair of scar tissue in patients after breast plastic surgery .Methods 66 patients with breast conserving surgery after breast conserving surgery were selected and divided into two groups.32 cases in the control group were treated with the implementation of breast conserving surgery and breast plastic surgery , 34 cases in the treatment group were treated on the basis of the control group and combined with Dermatix Ultra.Evaluation of patient and observer scar assessment scale ( POSAS ) and visual analogue scale were compared after the treatment and satisfaction degree were compared after 6 months follow-up.Results Compared with the control group, scar evaluation in treatment group were higher(P<0.05), the color, thickness, the degree of pigmentation and the score of the pigment were higher (P<0.05), the color, softness, thickness, pain and itching were higher (P<0.05), and the patients were followed up for 6 months (P<0.05).The color, thickness, roughness and pigmentation score of OSAS in the treatment group were higher (P<0.05), Color, softness, thickness, pain and pruritus in PSAS were higher (P<0.05),after 6 months of follow-up, the satisfaction rate in patients was higher (P<0.05).Conclusion Dermatix Ultra has a good clinical effect in repair of scar tissue after the breast plastic surgery, improve the color, thickness, smoothness and pigmentation of patients after breast augmentation surgery, and improve the prognosis.