Consumer Product Safety ; Drinking Water ; standards ; Equipment Safety ; Food Safety ; Public Health ; instrumentation ; methods ; Safety Management ; Water Supply

Environmental Monitoring ; methods ; Environmental Pollutants ; analysis ; Microchip Analytical Procedures

The treatments of infected bone defects are still a major clinical problem.In recent years,local delivery systems applied as anti-infection and bone repair material become a hot research topic.In this review,we discuss various influence factors such as osteoinductive growth factors,antibiotics,cartier material,extended release methods.We also introduce the recent progress in the development of local delivery system for the treatment of infected bone defects.

The enzymological characterizations of site-mutagenized rat recombinant DNA polymerase?, RQ182 and RQ183 were studied The phosphocellulose column chromatographies showed that the mutant and the wild DNA polymerases were all eluted by about 0.5 mol/ L KCI, but the denatured DNA-cellulose chromatographies showed that although the wild enzyme was eluted by 0.35 mol/L KCI, RQ182 and RQ183 were eluted by 0.55 and 0.45 mol/L KCI, respectively, indicating that the binding abilities to DNA of the mutant enzymes were increased. Km values for the substrate (dTTP)of the wild enzyme, RQ182 and RQ183 were determined as 38.5, 34.5 and 111.1 ?mol/L, respectively,and the Km values for the primer (oligo(dT)) were 1.28, 1.96 and 6.58 ?g/ml, respectively. The results showed that the affinities of RQ183 to the substrate and the primer were decreased dramatically. It is suggested that Arg182 and Arg183 were involved in the active site function of DNA polymerase ?, in binding to DNA template, in recognizing of primer, and in binding to and catalyzing of substrates of the enzyme.

It was pointed out in this paper that the nature of values was invisible knowledge by analyzing the rela-tion between values and invisible knowledge,and the measures were put forward for constructing the values in medi-cal staff in view of their value construction process and its influencing factors.

The induction of DNA single-strand breaks(ssb)by exposure to hematoporphyrin photosensitizer(PSD-007)followed by photoirradiation was observed in mice leukemia cells(L7712)in vitro. DNA ssb increased with the increase of dose of light in the rangeof 6.3-21 J/cm2 after treatment with 30?g/ml PSD-007. In lower concentration(1?g/ ml)of the drug,no ssb could be estimated after light exposure. Whan the concentration of PSD-007 was increased from 10 to 30?g/ml, however, the amount of ssb showed a rapid increase and tend to reach a plateau at 50ug/ml.comparing with ?-ray irradiation, we observed that PSD-007 could not increase the amount of ssb in cells irradiated with ?-ray and the most of ssb could be rejoined after incubation at 37℃ for 1 hour in the presence of PSD-007. However, DNA ssb induced by photosensitizer and light could not be repaired in post-photorad iated incubation. It is suggested that there exist some differences between the damages induced by photosensitization and by ioning radiation.From our experiment, we believe that DNA damage of cells is an early and critical event in photosensitization, which is different from that induced by y-ray irradiation.

【Objective】To investigate the relationship of plasma renin activity(PRA),angiotensin Ⅱ(Ang Ⅱ) and aldosterone(ALD) activities with syndrome patterns in patients with ventricular remodeling following myocardial infarction,and to supply neuroendocrine evidence for syndrome differentiation of ventricular remodeling following myocardial infarction.【Methods】Sixty patients with ventricular remodeling following myocardial infarction were classified as Qi stagnation and blood stasis(pattern 1,n=16),phlegm-turbidity blockage(pattern 2,n=15),yangQi deficiency(pattern 3,n=16) and yin-blood deficiency(pattern 4,n=13).Radioimmunoassay(RIA) was used to measure plasma renin,Ang Ⅱ and ALD levels in acute myocardial infarction patients 7 days after onset,and cardiac color ultrasound was used to examine left ventricular function.【Results】In patients with ventricular remodeling following myocardial infarction,left ventricular end diastolic diameter(LVEDD),left ventricular end diastolic volume(LVEDV),left ventricular end systolic volume(LVESV),and left ventricular ejection fraction(EF) increased as compared with those in the healthy.LVEDD,LVESV and LVEDV became larger in patients with pattern 3 and pattern 1 than those in patients with pattern 2 and pattern 4(P

Objective To establish a new method to identify and analyze Qingdai Powder and its composition.Methods X-ray Diffraction with Fourier pattern was used.Results The standard X-ray diffraction with Fourier pattern and characteristic diffraction peaks of Qingdai Powder and its composition drugs were obtained.Conclusion The powder X-ray diffraction method can be used as a simple and convenient method for the identification of Qingdai Powder and its composition drugs.

Objective To investigate the factors of influence in post-traumatic stress bleeding.Methods The clinical data of 1986 trauma patients and postoperative patients in recent 5 years were studied retrospectively,and cases with or without stress ulcer bleeding were comparatively analyzed.Results The incidence of stress ulcer bleeding in cases with serious injury was significantly higher than in those with mild injury(P

Along with the fast development of medical technology,the theory of holistic medical care is infiltrated into every aspect of clinical medical practices.In this paper,though the treatment of surgical hepatobiliary diseases under the direction of holistic medical care ,we emphasize the significance of holistic medical care in diagnosis and treatment ,recommend and canonize the usage of holistic medical care into the clinical practices.