1.A Commentary of Researches on College Students'Psychological Stress and Tackling Styles
Chinese Journal of Medical Education Research 2003;0(04):-
There are many researches on college students'psychological stress.We find that many students are facing pressures including study,social communication,job-hunting,economic problems and so on.There are also many researches on college Students'tackling styles.On the basis of a review on current researches,some suggestions for future work are also put forward.
2.The inhibitory effect of Rompun anesthesia and operation on immune function of dogs
Journal of Xi'an Jiaotong University(Medical Sciences) 2017;38(2):314-316
Objective To study the inhibitory effect of Rompun anesthesia and operation on immune function of dogs.Methods We randomly divided 14 healthy dogs into Group A and Group B with 7 in each.Rompun anesthesia induced by intramuscular injection of 3.0 mg/kg Rompun was carried out in Group A while Rompun anesthesia and operation were applied in Group B.The venous blood samples were collected before and after anesthesia at 2,8,24,72,120 and 168 h for centrifugation to obtain sera.ELISA kit was used to evaluate the contents of serum IL-1β,IL-2,IL-4,IL-8 and TNF-α.Results Compared with those before anesthesia,the concentrations of IL-2,IL-1βand TNF-αin Group A significantly decreased at 2,8,24 and 72 h (P<0.05)and resumed the pre-anesthesia level from 120 to 168 h;however,the concentrations of IL-4 and IL-8 did not change significantly during 2 to 168 h (P>0 .05 ).Compared with those before anesthesia,the concentrations of IL-2 and IL-1βin Group B significantly decreased from 2 to 168h (P<0.05),while the concentrations of TNF-αand IL-8 significantly increased (P<0 .05 ).IL-2 and IL-1βcontents in Group B from 2 to 168 h were significantly decreased compared with those in Group A (P<0.05 ),whereas IL-8 and TNF-αincreased significantly (P<0.05 ). Conclusion Both Rompun anesthesia and operation stress can inhibit dogs'immune function,and the latter has a stronger inhibitory effect than the former.
3.Compare the myocardial protection effect of HTK solution with blood cardioplegic solution during valve replacement surgery
Nan ZHANG ; Nan GUO ; Chuanming BAI ; Shutian SONG ; Jiewu ZHOU
Journal of Chinese Physician 2012;14(3):339-341
Objective To evaluate the effect of HTK solution on myocardial protection during valve replacement surgery.Methods 42 patients with rheumatic heart disease were randomized to receive 4∶1cold blood (control group,n =21 ) and HTK ( protective gronp,n =21 ) cardioplegic solution during valve replacement.The changes of CO and CI were collected at different time points including pre-operation,postoperative 6 hours,12 hours and 24 hours.Aortic clamping time,the ratio of spontaneous cardiac rhythm recovery and inotropic drugs application were calculated,and mechanical ventilation support time and the incidence of arrhythmia were recorded.Results The measurements of CO and CI showed that there was significant higher level in protective group at postoperative 12 hours and 24 hours [ 12 h:(4.82 ± 0.18 ) L/min vs ( 3.50 ± 0.32 ) L/min,( 3.80 ± 0.48 ) L/( min · m2 ) vs (2.79 ± 0.39) L/( min · m2 ) ;24 h:(4.97±0.45)L/min vs ( 3.81 ±0.19)L/min,(4.22±0.17)L/(min · m2) vs (2.91 ±0.21)L/(min·m2 ),P < 0.05].The clinical parameters including aortic clamping time,incidence of cardiac arrhythmia,inotropic support,duration of mechanical ventilation and length was lower than in control group [ (53.6 ±24.3 ) min vs ( 68.9 ± 26.1 ) min ; ( 1.8 ± 1.3 ) min vs ( 2.3 ± 1.2 ) min ; ( 33 ± 11 ) min vs ( 42 ± 13 ) min ;(10.2±2.1) μg/(kg · min) vs (15.7 ±3.8) μg/(kg · min);(14.6 ±4.8)h vs (20.7 ±5.1)h,P <0.05].The auto-beating rate was higher than in control group (90% vs 67%,P <0.05).Conclusions HTK solution is better than classical blood cardioplegia in myocardial protection during valve replacement.
4.Effect of cardura on benign prostatic hyperplasia and ejectile dysfunction
Yang LUO ; Qiong GUO ; Xiaoxin NAN
Chinese Journal of Geriatrics 2011;30(2):150-151
Objective To study the therapeutic effect of cardura on lower urinary tract symptoms (LUTS) and ejectile dystunction(ED) in men with benign prostatic hyperplasia (BPH).Methods One hundred and one cases with BPH and typical LUTS were investigated using the International Prostate Symptom Score (IPSS) and International Index of Erectile Function 5 (IIEF-5)before and after treatment. The patients were given cardura 4 mg, once a day for 8 weeks. The LUTS and ED were was analyzed after treatment. Results The IPSS was scores ( 14.3 ± 4.0) after treatment, and it was lower than before (mean scores: 19.9±6.5, t= 7.534, P<0.001). The IIEF2-5 was(14.9±8.8)scores after treatment, and it was higher than before (mean scores: 9.5±8. 5, t = 5. 335, P< 0. 001 ), respectively. There was statistically significant correlation between IIEF2-5 and IPSS score (r= -0. 327, P<0. 001 ). Various indexes were significantly improved after cardura treatment as compared with pretreatment (P<0.001). Conclusions LUTS is dangerous factor for sexual dysdysfunction, and the severity of LUTS is closely related to the development of sexual dysfunction. Cardura can at once improve the sexual function and LUTS of BPH patients.
6.Clinical significance of increased plasma IGF-1 in type 2 diabetic patients with malignant tumor
Yu CHEN ; Yingyu NAN ; Guo YE
Chongqing Medicine 2015;(5):628-630
Objective To test plasma insulin‐like growth factor 1(IGF‐1) level in type 2 diabetic patients with malignant tumor , and make comparison with diabetic patients and normal patients .To discuss the significance of detection of plasma IGF‐1 level in type 2 diabetes in screening early malignant tumor .Methods Plasma IGF‐1 level were determined in type 2 diabetes among malig‐nant tumor group ,type 2 diabetes ,and normal control group ,and statistical comparison was made between the three groups .Results the plasma levels of IGF‐1 of type 2 diabetes mellitus ,type 2 diabetes mellitus with malignant tumor were significantly lower than normal group(P<0 .05) .And the plasma level of IGF‐1 in type 2 diabetes mellitus with malignant tumor group was obviously high‐er than that of type 2 diabetic group(P<0 .05) .Plasma IGF‐1 ,FBG ,HbA1c and 2 HBG level are risk factors for tumor .The level of FIns is a protective factor for tumor .Conclusion Type 2 diabetes is closely related to the malignant tumor ,and the plasma levels of IGF‐1 is a risk factor for tumor .
7.Hepatic integrated backscatter in healthy rabbits
Yuemin NAN ; Ruijun GUO ; Xixian YAO
Chinese Journal of Ultrasonography 1993;0(04):-
Objective To measure hepatic ultrasonic integrated backscatter (IBS) in healthy rabbits as a non-invasive quantitative diagnostic means for hepatic steatosis, hepatic fibrosis, and liver cirrhosis; to determine the factors that may influence the IBS measurement. Methods Thirty-five healthy New Zealand white rabbits with normal liver function tests and normal liver biopsies were used in this study. HP Sonos 5500 image system with quantitative analysis function was used for IBS measurement. With the machine setting fixed, and optimal image obtained, left and right liver were divided into near, medial and far areas according to the depth. Further the measurements of IBS in different depth were performed respectively. Meanwhile, in the same liver the IBS image was collected and the change of IBS intensity was measured in various setting of machine index (MI), time gain compensation (TGC) and lateral gain compensation (LGC). Results Average image intensity (AII) of left liver (from near to far areas) was ( 25.38? 2.36)dB, ( 26.32? 2.38)dB, and ( 26.45? 2.28)dB, respectively. The AII of the right liver (from near to far areas) was ( 24.99? 2.23)dB, ( 24.92? 2.59)dB, and ( 25.38? 2.36)dB, respectively. The average standardized AII of image in left livers was significantly higher than that of the right (P
8.Surgical treatment for primary malignant pelvic tumors
Wei GUO ; Wanpeng XU ; Nan LI
Chinese Journal of Orthopaedics 2001;0(05):-
Objective To discuss the techniques of tumor resection, pelvic reconstruction and postoperative management by reviewing the patients with malignant pelvic tumors in recent years. Methods 76 patients with primary malignant pelvic tumors were treated operatively between July 1997 and July 2003. The series comprised 47 males and 29 females. 31 cases were diagnosed as chondrosarcoma, 15 as Ewing sarcoma, 7 as osteosarcoma, 3 as lymphoma, 3 as malignant fibrohistiocytoma (MFH), 1 as hemangiopericytoma, 2 as myeloma, 13 as giant cell tumor(GCT). According to Enneking's division, the most common region of the primary pelvic tumor involved was region Ⅱ (51 cases), followed by region Ⅰ (16 cases) and region Ⅲ (9 cases). 16 cases were reconstructed with threaded pins and cement after resection of the ilium. 9 patients had local resection of pubis and ischium. 21 patients had hemipelvectomy. Among 30 patients with periacetabular tumors, 8 were reconstructed with hemipelvic prosthesis, 7 with saddle prosthesis, 6 with replantation of cauterized tumor bone and total hip arthroplasty, 9 with cemented total hip replacement after curettage of lesion. Results After 16 months to 6 years follow-up, among the 21 patients with hemipelvectomy, 4 patients had local relapse because the sacrum invaded by the tumors. 7 of 25 patients with lesions in regionⅠ or region Ⅲ had local relapse postoperatively due to the marginal incision to the acetabula. Among the 21 patients who had tumor resection and reconstruction in region Ⅱ, 4 had local relapse, 3 of which reconstructed with cauterized hemipelvis. As for functional recovery, all of the 25 patients with lesions in region Ⅰand region Ⅲcould walk postoperatively without assistance of a cane. 21 patients with acetabular reconstruction after resection of lesions in region Ⅱ could sit and stand normally and walked with a cane, some of them even had normal gait. Conclusion The major principle of treatment for primary pelvic tumor is that en bloc resection, only then the functional reconstruction of pelvic ring can be considered. Operators should be acquainted with advantages and shortcomings of different reconstructive methods of acetabulum to prevent the complications.
9.Establishment of double-transfected MDCK Ⅱ cells expressing human organic anion transporting polypeptide 1B1 and multidrug resistance-associated protein 2 and identification of its functions
Fudan University Journal of Medical Sciences 2017;44(2):134-142
Objective To establish double-transfected Madin-Darby canine kidney (MDCK) [Ⅱ cells expressing human organic anion transporting polypeptide 1B1 (hOATP1B1) and multidrug resistanceassociated protein 2 (hMRP2)and to testify their functions,moreover,to study the transcellur transport of indoleamine 2,3-dioxygenase (IDO) inhibitor 1-methyltryptophan (1-MT) in the transfectants.Methods hOATP1B1/hMRP2 eukaryotic vectors pVITRO2-SLCO1B1-ABCC2 was obtained by genetic engineering method and then transfected into MDCK cells.Stably expressed MDCK cells were screened by using the geneticin G418.Real-time PCR,Western blot analysis and immuno fluorescent confocal microscopy were used to verify the proteins expression.Transport of the representative substrate pravastatin in different pH values and substrate concentrations and 1-MT were evaluated using the double transfectants.Results MDCK-OATP1B1/MRP2 was successfully established.Pravastatin displayed the optimal transcellular transport when pH value was 6.5.Transport of pravastatin demonstrated the concentration-dependent in the concertation range of 0) to 500 μmol/L.Transport of 1-MT showed no significant difference in MDCK cells and transfectants.Conclusions MDCK-OATP1B1/MRP2 was successful established;1-MT was not the substrate of OATP1B1 or MRP2 protein;and the eatablished double transfectant cell lines can be used to evaluate OATP1B1/MRP2-medicated transport of xenobiotics (e.g.new drug candidates) and endogenous compounds (e.g.bilirubin).
10.Experimental Study on Cold Preservation of Rat Liver with Panax Notoginseng Saponins
Nan JIANG ; Li LI ; Yongzhang GUO ;
Chinese Journal of Bases and Clinics in General Surgery 2003;0(02):-
Objective To investigate the effects and mechanism of Panax Notoginseng saponins (PNGS) on rat liver during cold preservation. Methods Using isolated perfusion of rat liver model (IPRL), Fura 2 method was used to measur the concentration of calcium ion in hepatic cells which had been preserved in DMEM solution with different concentration of PNGS added and cold preserved for 2 hours. Liver function, metabolic products of oxygen free radicals, energy substance and aucount of biliary flow as well as morphological study were measured from liver tissue which had been cold preserved in lactate riuges’s solution containing different dosages of PNGS for 24 hours and 30 minutes reperfusion.Results The contents of intracellur calcium of the rat hepatocytes,MDA, AST, ALT, LDH were lower than those in the control group,but SOD, ATP, TAN, EC and bile production were higher than those in the control group( P 0.05). Conclusion PNGS relieve the injury of the rat liver during cold preservation. The mechanism might through inhibition calcium overload, improve the energy metabolism, play a role against free radical injury realize.