It is high time to regulate the Chinese medical ethics review system and construct a supervision system of medical ethics review.According to the guideline of WHO,models of supervision system of medical ethic review in certain developed countries,and national conditions,this paper proposes several countermeasures including perfecting related legal system,constructing certification system of ethics review committee,and formulating integrated contents and procedure of supervision.

Objective To investigate the teaching effectiveness of preventive medicine and ask the students for feedback on the curriculum in order to reform the teaching methods.Methods Questionnaire survey.Sampling survey.Results 89.2％ of students have realized the importance of preventive medicine.Respectively 16.4％ and 7.5％ of students have no interesting on the curriculum due to teachers'matter and students' idea that the course is uselessness for their major and future career.75.1％ of students are interest in Nutrition and Food Hygiene,35.2％ of students dislike Medical Statistics,and 45.3％ of students think the most tough part is Medical Statistics.78.9％ of students wish to have more practice lessons.30.5％ of students do not ask teachers questions because of no communication with teachers after class.Conclusions Preventive Medicine is still ignored by a few students.The more tough the content is,the less interest the students have. Repeatedly emphasizing the importance of Preventive Medicine in medical education,adopting and combining with the community practical cases in class lectures and frequently communicating between teachers and students after class are suggested.

We observed in a pilot study that there was a transient elevation of brain natriuretic peptide (BNP) level shortly after the transplantation in the patient with ischemic heart failure, which is unexplainable by the simultaneous increase of the cardiac output and six-minute walk distance. Similar findings were observed in the phase I trial. We postulated on the basis of the finding of Fukuda in vitro that this transient elevation of BNP level against the improvement of cardiac function and exercise capacity might indicate cardiomyogenesis in patients after mesenchymal stem cell transplantation. Further study is warranted to verify the hypothesis.
Adult ; Aged ; Female ; Humans ; Male ; Mesenchymal Stem Cell Transplantation ; Middle Aged ; Muscle Development ; Myocytes, Cardiac ; physiology ; Natriuretic Peptide, Brain ; physiology ; Transplantation, Autologous ; Ventricular Remodeling

The paper expounds the importance and role of circulation service in college and university libraries, how to do circulation work better is discussed from three aspects: scientific management of collections, creating a better environment and fully promoting the quality of staffs, thus to make library play a greater role in campus culture construction.

Objectlve To explore the effect of platelet-rich plasma (PRP) on cellular proliferation and intracytoplasm calcium ion concentration of osteoblasts and tenocytes during the process of tendon-bone healing. Methods We established a kind of indirect co-culture system using transwell chambers to co-culture osteoblasts and tenocytes.The cellular proliferation was carried out in following manners:single culture of osteoblasts versus single culture of osteoblasts plus PRP and co-culture of osteoblasts versus co-culture of osteoblasts plus PRP; single culture of tenocytes versus single culture of tenocytes plus PRP and co-culture of tenocytes versus co-culture of tenocytes plus PRP.The cellular proliferation rates were measured by CCK-8 test.The intracytoplasm calcium ion concentration of was measured by laser confocal microscopy and fluo-3/AM. Results The proliferation rates and fluorescence intensities in the co-culture groups without PRP were significantly lower than other groups ( P ＜ 0.05).The proliferation rates and fluorescence intensities in the single culture groups without PRP were significantly higher than in the co-culture groups without PRP but significantly lower than in the groups with PRP ( P ＜ 0.05).The proliferation rates and fluorescence intensities in the groups with PRP were significantly higher than other groups ( P ＜ 0.05) but there was no significant difference between groups of the same cells ( P ＞ 0.05).The intracytoplasm calcium ion concentrations were proportional to the proliferation rates. Conclusions During the process of tendon-bone healing,PRP has a potential not only to wipe off the depression effect of osteoblasts and tenocytes co-cultured indirectly but also to enhance the cellular proliferation rate to a higher level.At the same time the calcium ion concentrations will be elevated.

Objective To observe the clinical efficacy of cluster needling at the anterior oblique line of vertex-temporal plus speech training in treating motor aphasia after cerebral infarction.Method Forty patients with motor aphasia after cerebral infarction were randomized into a treatment group and a control group, 20 in each group. Both groups were given basic neurological treatment. In addition, the treatment group was intervened by cluster needling at the anterior oblique line of vertex-temporal plus speech training, while the control group was by speech training alone. After 4-week treatments, the speech function and communication ability were observed and the clinical efficacies were compared.Result The total effective rate was 90.0% in the treatment group versus 80.0% in the control group, and the difference was statistically significant (P<0.05). After treatment, the speech function score and Chinese Functional Communication Profile (CFCP) score were changed significantly in both groups (P<0.01). There were significant differences in comparing the speech function and CFCP scores between the two groups (P<0.01,P<0.05).Conclusion Cluster needling at the anterior oblique line of vertex-temporal plus speech training is an effective approach in treating motor aphasia after cerebral infarction.

Objective To construct the human Alzheimer's disease (AD) specific single chain anti‐bodies (scFv) library for sceening human AD scFv to Aβ1‐42 oligomers .Methods RNA was isola‐ted from 40 ml peripheral blood taken from 18 AD patients .Variable heavy (V H ) and variable light (VL ) genes were amplified by RT‐PCR and linked to the scFv fragments that were then cloned into the phage vector pCANTAB5E .The scFv library was constructed by electroporating E .coli TG1 cells into the pCANTAB5E and rescuing the assistant phage M13K07 .Results The total RNA was extracted .The VH and VL genes were amplified .Electropharesis showed that the length of VH and VL genes was 360bp and 300bp respectively and the length of linked scFv was 750bp .The 2 .4 × 109 scFv library was thus constructed and identified by BstN I digestion .Elec‐tropharesis showed that the length of BstN I‐digested scFv fragments varied ,indicating that the library is of a good diversity .Conclusion The human AD phage scFv library we constructed lays a foundation for screening the antibodies to Aβ1‐42 oligomers and the treatment of AD .

It was pointed out in this paper that the nature of values was invisible knowledge by analyzing the rela-tion between values and invisible knowledge,and the measures were put forward for constructing the values in medi-cal staff in view of their value construction process and its influencing factors.

Objective To investigate analgesia of dezocine in abdominal anesthesia postoperative.Methods 80 cases (ASA I ~II)abdominal surgery under general anesthesia patients required postoperative analgesia,according to different postoperative analgesia drug,patients were randomly divided into 2 groups:dezocine group (group D),dezocine 0.6 mg/kg+ondansetron 4mg plus 0.9% sodium chloride to 100 mL,loading dose of intravenous patient controlled analgesia (PCIA);fentanyl group (group F),fentanyl 12 g/kg+ondansetron 4mg plus 0.9% sodium chloride to 100 mL,loading dose plus PCIA,recorded after 2,4,8,12,24,48 h visual analog scale (VAS)and Ramsay sedation score.Results The 2 groups were not statistically significant differences in VAS score and Ramsay score,and the incidence of adverse reaction of fentanyl group was significantly higher than that of dezocine group (P<0.01).Conclusion Dezocine group could provide analgesia safe and comfortable for patients after abdominal operation under general anesthesia.

OBJECTIVE:To establish the quality standard for Gardenia jasminoides. METHODS:The moisture,total ash and extract were determined. HPLC was used for contents determination of geniposide,rutin,crocinⅠand crocinⅡ:the column was Waters Xbridge-C18 with mobile phase of acetonitrile-0.2% phosphoric acid(gradient elution,gardenia and rutin),methanol-water (45∶55,V/V,crocinⅠ,crocinⅡ)at a flow rate of 1.0 ml/min;detection wavelength was 256 nm for gardenia and rutin,440 nm for crocinⅠ,crocinⅡ;column temperature was 30℃;injection volume was 10μl for gardenia and rutin,5μl for crocinⅠ,cro-cinⅡ. RESULTS:The moisture total ash and ethanol-soluble extract of G. jasminoides were 5.8%-8.4%,3.7%-5.9% and 29.5%-37.9%,respectively. The linear range was 162.08-1 620.84 μg/ml for geniposide(r=0.999 9),2.07-20.72 μg/ml for rutin (r=0.999 9),8.04-80.41 μg/ml for crocinⅠ(r=0.999 9)and 1.05-10.53 μg/ml for crocinⅡ(r=0.999 9);RSDs of precision,sta-bility and reproducibility test were lower than 2%;recoveries were 99.33%-101.43%(RSD=1.09%,n=6),97.97%-101.83%(RSD=1.39%,n=6),97.97%-101.30%(RSD=1.36%,n=6) and 98.65%-103.04%(RSD=1.84%,n=6). CONCLUSIONS:The established standard can be used for the quality control of G. jasminoides commercially available.