An objective assessment of stress is needed to manage stress and prevent the development of stress-related diseases such as depression and coronary heart disease. In this paper, we discussed the possibility of applying saliva to an assessment of stress. We illustrated the procedures for collecting and analyzing saliva, and reviewed the relationships of acute, chronic, psychological, or physical stress to seven salivary substances: Cortisol, Dehydroepiandrosterone, Testosterone, Chromogranin A, 3-Methoxy-4-hydroxyphenyglycol, α-Amylase, and Secretory Immunoglobulin A. Considering unique characteristics of each salivary substance and purposes of the study or the non-invasive assessment, salivary substances should be selected for the assessment of stress.
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