100 ?g/mL, respectively. Conclusion Compounds Ⅰ-Ⅴ are isolated from B. parviflora for the first time, Ⅲ is a new phenolic glucoside named as bidenphenol glucoside. Compounds Ⅰ-Ⅴ exhibit the activities of anti-histamine release from rat mast cell stimulated by antigen-antibody reaction.

Objective Based on the activities of antihistamine release to study the compounds from Bidens parvi-flora and find biological active compounds. Methods The chemical constituents from B.parviflora were isolated by silica gel and Sphadex LH-20 column chromatographies and purified by preparative HPLC. The chemical structures had been identified by physiochemical properties and spectroscopic methods. Results Six caffeoylquinic acid derivatives were identified as 3, 5-di-O-caffeoylquinic acid ( Ⅰ ),3, 4-di-O-caffeoylquinic acid ( Ⅱ ), 4, 5-di-O-caffeoylquinic acid ( Ⅲ ), 4-O-caffeoylquinic acid ( Ⅳ ), 5-O-caffeoylquinic acid ( Ⅴ ), 4-[3-(3, 4-dihydroxy-phenyl)-acryloyloxy]-2, 3-dihydroxy-2-methyl-butyric acid ( Ⅵ ). Conclusion Compounds Ⅰ - Ⅵ are first obtained from B. parviflora and Ⅵ is new one. Some of the compounds exhibit the activities in antiallergic assays. Moreover, the structure-activity relationships of these compounds have been also discussed in this paper.

Objective To study the chemical constituents from the stems of Dendrobium nobile. Methods Compounds were isolated through various chromatographic techniques and identified by spectral data. Results Twelve phenolic compounds were obtained. Their structures were characterized as dihydroconiferyl dihydro-p-coumarate (Ⅰ), vanillin (Ⅱ), apocynin (Ⅲ), p-hydroxybenzaldehyde (Ⅳ), syringaldehyde (Ⅴ), syringic acid (Ⅵ), syringylethanone (Ⅶ), α-hydroxypropiosyringone (Ⅷ), coniferyl aldehyde (Ⅸ), dihydroconiferyl alcohol (Ⅹ), 2-hydroxyphenylpropanol (Ⅺ), and 3-hydroxy-4-methoxyphenylethanol (Ⅻ), respectively. Conclusion All above compounds are reported from this plant for the first time. Compounds Ⅰ and Ⅲ -Ⅻ are reported for the first time from the plants of Dendrobium Sw.

Aim To investigate the water-soluble phenolic glycosides from the whole plant of Bulbophyllum odoratissimum. Methods Column chromatography techniques were used to isolate the chemical constituents, physico-chemical constants and spectroscopic analysis were employed for structural elucidation. Results Bulbophyllinoside (1), a new phenolic glycoside and three known compounds were isolated from the whole plant of Bulbophyllum odoratissimum Lindl. Their structures were determined as 3-hydroxyphenethyl alcohol 4-O-(6'-O-β-apiofuranosyl)-β-D-glucopyranoside (1), 3-methoxyphenethyl alcohol 4-O-β-D-glucopynanoside (2), 3,5-dimethoxyphenethyl alcohol 4-O-β-D-glucopynanoside (3) and syringin (4). Conclusion Bulbophyllinoside (1) is a new compound.

AIM　To investigate the structures and immunomodulation activity of four homogeneous polysaccharides: LBP 1a-1, LBP 1a-2, LBP 3a-1 and LBP 3a-2 isolated from Lycium barbarum L. brought from Zhongning County, Ningxia Province. METHODS　Their molecular weights, sugar component (constituents) and their linkages were determined by gel permeation chromatography, acid hydrolysis, periodate oxidation and NMR spectrum. The activity of immunomodulation was evaluated with splenocyte proliferation by ［3H］-TDR incorperation, in vitro. RESULTS　Four polysaccharides with molecular weights 11.5×104, 9.4×104, 10.3×104 and 8.2×104, were shown to enhance splenocyte proliferation induced by ConA. LBP 1a-1 and LBP 1a-2 were α-(1→6)-D-glucans. LBP 3a-1 and LBP 3a-2 were found to be α-(1→4)-D-polygalacturonans. CONCLUSION　The four polysaccharides were first isolated from this plant. Polysaccharides with main chain of α-(1→4)-D-polygalacturonans showed stronger immunomodulation activity.

In our study, we adopted a schedule plan management of the Project Management Body of Knowledge(PMBOK)in the management practice of the expefimental research on "The Research in Teaching and Inheritance of Experience of Famous and Veteran Doctors of TCM "which was supposed by " tenth five years "plan of national science and technology as a key program. We tried to establish a project planning based on the special features of TCM and the characters of the project. This planning played a significant support role in the top project design and ensuring the smooth implementation of the research.

In our study,we adopted a schedule plan management of the Project Management Body of Knowledge(PMBOK)in the management practice of the experimental research on "The Research in Teaching and Inheritance of Experience of Famous and Veteran Doctors of TCM " which was supported by "tenth five years" plan of national science and technology as a key program.We tried to establish a project planning based on the special features of TCM and the characters of the project.This planning played a significant support role in the top project design and ensuring the smooth implementation of the research.

OBJECTIVETo investigate the anti-proliferative effect of Protobioside on the HepG2 cells and its mechanism.

METHODThe inhibitory effects of Protobioside on 3 kinds of human cancer cell line was measured by MTT assay. Apoptotic morphological changes was observed by Hoechst33528 staining technique. Cell cycle were analyzed using flow cytometry. The expression of cell cycle related protein and apoptosis related protein were determined using Western blotting.

RESULTProtobioside shows strong cytotoxic power on HepG2 cells and IC50 were 20 micromol x L(-1). The nucleus became pyknosis at 36 hours. The cells in G2/M phase increase in a dose-dependent and time-dependent manner. And the protein level of CyclinB1 was down-regulated, that of Bax was up-regulated and that of Bcl-2 was down-regulated.

CONCLUSIONThe growth inhibition of Protobioside on HepG2 cells is highly related to cell cycle arrest at G2/M phase which was well correlated with the down-regulation of expression of Cyclin B1, and the induction of apoptosis via up-regulating of Bax and down-regulating Bcl-2 expression.

Apoptosis ; drug effects ; Blotting, Western ; Cell Cycle ; drug effects ; Cell Line, Tumor ; Cell Proliferation ; drug effects ; Drugs, Chinese Herbal ; pharmacology ; Flow Cytometry ; Hep G2 Cells ; Humans

OBJECTIVETo study the chemical constitutents of the 60% ethanol extract of the stems of Sambucus williamsii.

METHODCompounds were isolated and purified by Diaion D101, silica gel,Sephadex LH-20, ODS column chromatography and preparative HPLC. Their structures were identified by spectroscopic methods.

RESULTSeven lignans were isolated and identified as erythro-guaiacylglycerol-beta-O-4'-sinapyl ether (1), 1-( 4'-hydroxy-3'-methoxyphenyl) -2- [4"-( 3-hydroxypropyl) -2", 6"-dimethoxyphenoxy] propane-1,3-diol (2), isolariciresinol (3), burselignan (4), lyoniresinol (5), 5-methoxy-isolariciresinol (6), cycloolivil (7).

CONCLUSIONAll these compounds were obtained from this genus for the first time.

Anisoles ; chemistry ; isolation & purification ; Antineoplastic Agents, Phytogenic ; chemistry ; isolation & purification ; Chromatography, High Pressure Liquid ; Lignans ; chemistry ; isolation & purification ; Lignin ; chemistry ; isolation & purification ; Microscopy ; Naphthalenes ; chemistry ; isolation & purification ; Naphthols ; chemistry ; isolation & purification ; Plant Stems ; chemistry ; Sambucus ; chemistry