1.Determination of Lead and Ferrum in Pre-mixture Coating Film by Atomic Absorption Spectrometry
Ying YANG ; Yan JIANG ; Naijiang CHEN
China Pharmacist 2015;18(10):1805-1807
Objective:To establish an atomic absorption spectrometry method for the determination of lead and ferrum in pre-mix-ture coating film. Methods:The samples were digested by microwave digestion system, and Pb was determined by graphite atomic ab-sorption spectrometry(GFAAS), and Fe was determined by flame atomic absorption spectrometry(FAAS). Results: There was good linear relationship within the range of 1.0-5.0 μg·L-1(r=0.999 4) for lead and 0.1-1.0 mg·L-1(r=0.998 4) for ferrum. The recovery of lead was 91. 3%(RSD=1. 4%,n=9)and that of ferrum was 89. 9%(RSD=3. 5%,n=9). Conclusion: The method is accurate and stable, which can be used for the determination of lead and ferrum in pre-mixture coating film
2.Determination of Three Bacteriostatic Agents in Taurine Eye Drops by HPLC
Ying YANG ; Rui MAO ; Naijiang CHEN
China Pharmacist 2017;20(9):1697-1699
Objective: To establish an HPLC method for the determination of three bacteriostatic agents ( methylparaben, eth-ylparaben, propylparaben) in taurine eye drops. Methods:The HPLC method was conducted on a Phenomenex Gemini C18 column ( 250 mm × 4. 6 mm, 5 μm ). The mobile phase was 1% acetic acid-methanol(40 :60). The flow rate was 1. 0 ml·min-1 and the de-tection wavelength was 254 nm with the injection volume of 20 μl and the column temperature of 25℃. Results:Methylparaben, eth-ylparaben and propylparaben showed good linear relationship (r>0. 999)within the range of 1. 00-19. 94μg·ml-1,2. 01-20. 08μg· ml-1 and 0. 21-10. 46 μg·ml-1,respectively. The average recoveries were 99. 20%-99. 90%, and the RSDs were 1. 34%-1. 54%(n=9). Conclusion:The method is accurate, reproducible and stable without interference, which can be used for the determination of three bacteriostatic agents in taurine eye drops.
3.Determination of Potassium Sorbate in Xiao'er Kechuanling Oral Solution by HPLC
Ling LI ; Cheng CHENG ; Naijiang CHEN
China Pharmacist 2018;21(1):157-158
Objective:To develop a method for the determination of potassium sorbate in Xiao 'er Kechuanling oral solution by HPLC.Methods:A Phenomenex Gemini C18 column (250 mm ×4.6 mm, 5 μm) was used, and the mobile phase was 0.02 mol· L-1 sodium dihydrogen phosphate (adjusting pH to 4.0 with phosphoric acid)-acetonitrile (80:20).The flow rate was 1.0 ml· min-1 and the detection wavelength was set at 262 nm.The column temperature was 30℃and the injection volume was 20μl.Results:The linear range of potassium sorbate was 8.584-85.840μg· ml-1(r=0.9999).The recovery of potassium sorbate was 100.0%and the RSD was 0.47%(n=9).The limit of detection was 0.43 ng and the limit of quantitation was 1.29 ng.Conclusion:After the valida-tion, the method can be used for the assay of potassium sorbate in Xiao'er Kechuanling oral solution .