Human gastric cancer cell line MKN28 and BGC823 were infected with recombinated retroviruses containing TNF-? gene, The expression and function of TNF were identified in transfected cancer cell line MKN28-TNF and BGC823-TNF. Cancer cells showed lower growth rate after transfection, and the inhibition of growth rate were 71% and 66% in MKN28-TNF and BGC823-TNF in comparison with the control, respectively. Decreased levels of ~3 HTdR incorporation were observed. The tumorigenicity of MKN28-TNF in nude mice was completely lost as compared with the control . The results demonstrate the inhibition of gastric cancer cells growth and tumorigenicity introduced by exogenous TNF gene.

Acupuncture Therapy ; Analgesia ; Animals ; Brain Chemistry ; Morphine ; pharmacology ; Opium ; analogs & derivatives ; antagonists & inhibitors ; Rats