Objective To study the application efficacy of ultrasound-guided catheter lavage in patients with severe pancreatitis surgery. Methods 60 patients with severe pancreatitis from January 2009 to March 2013 in our hospital were randomly and equally divided into the experimental group and the control group. Patients of the control group were merely treated with comprehensive treatment after severe acute pancreatitis operation,and Patients of the experimental group were given ultrasound-guided catheter lavage treatment additionally. Hospital stays,clinical examination indicators and clinical effects of the two groups were analyzed. Results Hospital stay of experimental group was significantly shorter than the control group (P<0. 05);the temperature and heart rate of experimental group was significantly lower than con-trol group (P<0. 05);leukocyte,serum amylase and urine amylase of the experimental group were significantly lower than the control group (P<0. 01). The total effective rate of experimental group (93. 3%) was significantly higher than the control group (66. 7%),which means a statistically difference (P<0. 01). Conclusion Ultrasound-guided catheter lavage has good results for patients with severe pancreatitis surgery,and it is worthy of clinical application.

Aim Toinvestigatetheeffectsoftotalsap-onin of Dioscorea (TSD)on rats with monosodium u-rate crystal-induced acute gouty arthritis (AGA)and mechanisms.Methods Totally72Wistarratswere randomly devided into six groups,Each group was giv-en corresponding drug before,then rat acute gouty ar-thritis model was made by injection of monosodium u-rate in the ankle joint cavity.The gait,articular swell-ing degree and physiological changes of rats were ob-served.The concentration of TNF-α,IL-1β,IL-18 in serum were detected by ELISA.The levels of pro-IL-1β, NALP3, ASC, pro-caspase-1, and cleaved caspase-1 were detected by Western blot.Results AllTSDgroupsandcolchicinesignificantlychangedthe gait of rats and TSD high and middle groups signifi-cantly reduced joint swelling and diminished the patho-logical changes.The levels of TNF-α,IL-1β,IL-18 in serum were significantly decreased,and the levels of pro-IL-1β,NALP3,ASC,pro-caspase-1 and cleaved caspase-1 were apparently reduced in TSD high and middlegroups.Conclusion TSDpossessesanti-goutfunction and the mechanism may be related to sup-pressing the NALP3 inflammasome activation and in-hibiting the cytokine production.

Background Dacryocystitis is one of the most common infectious eye diseases.The gold standard for the identification of bacteria causing dacryocystitis is bacterial culture.The combination of regular culture method with molecular biology techeniques will generate more reliable results.However,very few research data are available in ophthalmological studies in this area.Objective This study was to identify the genera and species of the dacryocystitis-causing bacteria by PCR amplification of the 16S rRNA sequences.Methods Ten cases of qualified standardized bacteria samples were taken,and the nucleic acids were released in the heating process of the PCR procedure.The 16S rRNA genes were amplified and sequenced,and the genera and species were identified using BLAST from GenBank,and the results were used to compare with the results from biochemical identification to test the reliability of this method.The cultured bacterial species from the lacrimal sac secretions from 30 cases of dacryocystitis patients were identified with the above method.Results The outcome of the PCR identification for the 10 cases of quality control standard bacterial specimens was consistent with the results from the biochemical identification.The identification of the 30 cases of dacryocystitis through sequencing the 16S rRNA revealed there were 13 cases of Staphylococcus epidermidis infection,2 cases of Staphylococcus warneri infection,1 case of Staphylococcus hominis infection,5 cases of Corynebacterium macginleyi infection,3 cases of Streptococcus pneumonia infection,2 cases of Bacillus cereus infection,1 case of Micrococcus luteus infection,1 case of Moraxella catarrhalis infection,1 case of Moraxella osloensis infection and 1 case of Pseudomonas aeruginosa infection.Conclusions Sequencing the 16S rRNA is an accurate and specific way for the identification of the genera and species of bacteria that cause dacryocystitis in patients.This sequencing method is feasible in monitoring a variety of dacryocystitis-causing pathogens.More information and epidemiological statistics about dacryocystitis can be obtained from 16S rRNA sequencing.

Two Chinese versions of the Hearing In Noise Test (HINT) were developed for assessing speech understanding ability of Cantonese and Mandarin speakers - the Cantonese Hearing In Noise Test (CHINT) and the Mandarin Hearing In Noise Test (MHINT). A total of 168 subjects with normal hearing thresholds participated in the studies. Sentence materials that represent daily conversational speech were created and recorded. These sentences were equated for difficulty by adjusting their presentation levels in noise. Twelve 20-sentence lists with equal phonemic content were created. Using these lists, Reception Thresholds for Sentences (RTSs), defined as the signal-to-noise ratio at which sentences were repeated correctly 50 % of the time,were obtained. RTSs were measured under headphones in quiet and in three noise conditions. For the noise conditions,speech was presented from the front and noise was processed to simulate origination from the front,the right and the left. The noise was fixed at 65 Db (A) and the speech level was adjusted adaptively. Interlist or test-retest reliability was high,suggesting that consistent results can be obtained using any list. Confidence intervals were measured and reported. Norms obtained using the CHINT and MHINT are comparable to those for the English HINT.

The number of papers published in American Journal of Pharmaceutical Education and Chinese Journal of Pharmaceutical Education from 2003 to 2013 was calculated .The knowledge maps of research hot spots and co-operation maps of research institutions in the two journals were plotted .The different research-oriented key points and the cooperative relationship between different research institutions in the two journals and the problems existed in domestic journals of pharmaceutical education were analyzed with certain suggestions put forward for the solution of the problems existed in domestic journals of pharmaceutical education .

Objective To discuss the electrocardiogram(EKG)positioning method to the guiding role of determining the pipe length and the accuracy of operative localization in central venous catheterization procedure. Methods Chose 32 cases of tumor patients who had center venipunture.Use the catheter taken EKG data when cath-etering,and then given validation using postoperative chest X -ray or fluoroscopy.Judgment the sensitivity,specificity and disposable catheters success rate of the EKG positioning method.Results In the 32 cases of cancer patients, 30 patients had characteristic P waves,when the chest X -ray confirmed the superior vena cava or the junction with the right atrium,one case into the right atrium,when one case of non -P -wave in the subclavian after intravenous discounts tune into the tube after it confirmed the superior vena cava.Conclusion EKG positioning method with high accuracy in the deep venous catheter in the catheter tip positioning applications.The clinical applications are feasible.

Objective To analyze the chemical constituents of kumquat essential oil(KEO)and the acute toxicity of KEO in mice. Methods The chemical constituents of KEO were analyzed by GC-MS. After preliminary test, KM mice were randomly divided into control and KEO groups with 20 mice in each group(10 male and 10 female). The doses of KEO were designed with ratio 0.758 between the 100% and 0% mortality amount as 10, 7.60, 5.70, 4.40, 3.30 and 2.50 ml/kg. The acute toxicity was evaluated by examination of the mice for 14 days after single administration of KEO. Results The principal constituents in KEO were α-pinene(0.46%), β-phellandrene(0.11%), β-myrcene(2.34%), D-limonene(95.62%), geranyl acetate(0.10%), β-copaene(0.67%), and γ-elemene (0.13%), which accounted for 99.43% of the total chemical content of KEO. The half-lethal dose(LD50)of KEO in mice by the acute toxicity test was 6.24 ml/kg with the 95% confidence limit of(5.39-7.33)ml/kg in female mice and 5.73 ml/kg with the 95% confidence limit of(4.91-6.79)ml/kg in male mice. The possible toxic target organs were liver, spleen and gastrointestinal tract. Conclusion KEO with the content of D-limonene was obtained from kumquat for the first time, and the toxicity of the KEO was low according to the toxicity classification standard of World Health Organization.

AIM: To determine adenosine content in Kudiezi Injection(Ixeris sonchifolia Hance). METHODS: HPLC was used with Shim-pack CLC-ODS(6.0 mm?150 mm,5 ?m). The mobile phase consisted of methanol-acelonitrile-0.025 mol?L -1 phosphoric acid (3∶4∶93). The flow rate was 1 mL?min -1 . The UV detection wavelength was at 260 nm. RESULTS: The adenosin content in Kudiezi Injection was 0.15/10 mL. The average recovery was 98.4%(n=5). CONCLUSION: HPLC can be used for the quality control of Kudiezi Injection Injection.

BACKGROUND:Transplantation of autologous stem cells for treatment of liver cirrhosis has been widely reported.But up to now,there exist some concerns for clinical physicians,including relationship between stem cells and post-transplantation prognosis/turnover of liver cirrhosis,directed differentiation of stem cells in the impaired liver,and malignant phenotype.OBJECTIVE:To dynamically monitor the serum levels of alpha-fetoprotein(AFP)and AFP variants(AFP-L3)in decompensated cirrhosis patients following intrahepatic transplantation of peripheral blood stem calls via portal vein and evaluate the safety of this treatment method.METHODS:A total of 44 decompensated cirrhosis patients who underwent intrahepatic transplantation of peripheral blood stem cells via portal vein in the 309 Hospital of Chinese PLA in April 2007 were included in this study.Prior to and after surgery,serum levels of AFP and AFP-L3 were detected by chemiluminescence.Through the use of a positive criterion for liver cirrhosis,i.e.,the proportion of AFP-L3 in AFP[AFP-L3(%)]≥10%,and the relationship between decompensated cirrhosis treatment using stem cells transplantation and the malignant phenotype of liver cancer were analyzed.RESULTS AND CONCLUSION:At 2 months after surgery,serum level of AFP showed a transient increase.There was no significant difference in AFP-L3(%)between prior to and after surgery(P>0.05).No significant difference in AFP-L3-positve rate,as well as AFP-L3(%),existed among patients with different serum level of AFP.These findings indicate that clinical symptoms and liver function of decompensated cirrhosis patients recovered to some extent after transplantation of peripheral blood stem cells via portal vein.Results regarding serum level of AFP-L3,a serological marker of liver cancer,did not demonstrate the appearance of malignant biological phenotype.

Humans ; Neck Injuries ; therapy ; Vertebral Artery ; injuries ; Vertebrobasilar Insufficiency