Objective The physiological and biochemical changes were studied during the Panax notoginseng′s fruit development.Methods Dynamic changes of fruits size,fresh and dry weight,moisture content and soluble sugar,starch and protein contents were investigated.Results The moisture content was increased first and then dropped to 73.37% at maturity.The fresh and dry weight,starch and protein contents of P.notoginseng′s fruits were increased with the fruit development.The soluble sugar content was raised first,then decreased,and increased slightly at the lateral stage of fruit development.Conclusion The P.notoginseng′s fruits are mature at 80 d after peak anthesis;the decrease of moisture content is companied with the gradual increasing of fruit fresh and dry weight during the fruit development.The storage and utilization of nutrients in P.notoginseng′s fruits are closely related with the fruit development.

The impact of the pandemic, caused by SARS-CoV-2, on the management of malignancies has posed several challenges. Gestational trophoblastic diseases, being considered highly curative, are categorized as emergent cases which need immediate intervention with a level I priority for chemotherapy for its malignant spectrum. These guidelines have been drawn up to assist in decision-making and the implementation of such are dependent on the capabilities of each institution combined with their local experience.

Objective To develop and verify a plaque method for detection of infectious titer of tick-borne encephalitis virus(TBEV)strain(PHKT strain for short)adapted to primary hamster kidney(PHK)cells.Methods PHK cells were infected with TBEV,a primary mouse brain adaption strain,and passed consecutively for 12 passages.The titer of PHKT was detected by plaque method(Monolayer BHK-21 cells were infected with PHKT of various passages at different dilution ratios,and the plaque number was calculated by neutral red staining)and challenge titration in mouse brain(Mice were challenged with PHKT of various passages at different dilution ratios through brain cavity,0.03 mL for each,observed continuously for 14 days,and calculated for the median lethal dose(LD50)by Reed-Muench method)respectively,and the correlation between the results of two methods was analyzed.The developed plaque method for the detection of TBEV titer was verified for specificity,repeatability and intermediate precision.Results The plaque titer of PHKT virus was up to8.9 lgPFU/mL;The correlation between the results of plaque method and mouse brain challenge titration method was good(r = 0.92);The specificity of plaque method for detecting infectious titer of PHKT virus was good,and the coefficients of variation(CVs)of repeatability and intermediate precision were both less than 5%.Conclusion A plaque method for detecting infectious titer of PHKT virus was developed,which may be used as an alternative method for challenge titration in mouse brain.

Myelodysplastic syndromes (MDS) is a highly heterogeneous group of clonal disease,which is derived from hematopoietic.stem cells.Anemia is the main manifestation of most MDS patients and transfusion is required in about 90 ％ patients.Long-term blood transfusions can lead to iron overload (IOL).IOL can damage the iron balance,and excess free iron can deposite in the heart,liver and other vital organs,resulting in structural damage and organ dysfunction,which can deteriorate the prognosis,accelerate the transformation into acute myeloid leukemia,and increase the mortality.Iron chelation can improve the prognosis of patients with IOL,and prolong the overall survival and leukemia-free survival.

Autophagy is a lysosomal degradation of intracellular material composition,and plays an important role in nerve cell survival and clear senescent cells and misfolded proteins.Autophagy can protect nerve cells,but also can be used as one of the ways about nerve death.Repeated seizures can cause brain damage,but the specific mechanism is not clear.Recent studies have shown that,convulsion can activate apoptosis pathway and start autophagy,which can mediate the adaptation,injury or apoptosis of neurons.Convulsion brain injury is closely related to cell autophagy.Autophagy as a new important signaling pathway can provide a new therapeutic direction of convulsion brain damage.

The internet addiction disorder is an important problem under the internet circumstance.Improving self-regulated ability is a good method to avoid the problem.The article uses the selfregulated circle theory to gain the self-regulating strategy in three phases:forethought,performance or volitional control,self-reflection.These strategies include making goal,planning strategy,self-observation and self-record,attribution.

Thermochemotherapy by drug-loaded magnetic micro/nano-particles is attracting increasing attention because it combines the advantages of chemotherapy and high targeting and selectivity of hyperthermia,especially intra-cellular hyperthermia.Magnetic micro/nano-particles,usually consisting of biodegradable pely-mers matrics co-incorporated with ferromagnetic particles and anti-cancer drugs,will generate heat when exposed to an externally applied alternating magnetic fields due to electromagnetic induction by ferro-particles,in the meanwhile,sensitivity of anti-cancer drug will be greatly enhanced by higher temperature.Tumors will greatly suppressed by the synergetic effect of chemotherapy and targeted hyperthermia.

The development of effective therapy for children with acute lymphoblastic leukemia (ALL) is one of the greatest successes of clinical oncology. However, cure rates for adults with ALL remain relatively low. Adolescents are the special population between children and adults. These patients group will be received protocol from either pediatric or adult oncologists depending on referral pattern. In recent years, researchers from abroad large cooperative groups show pediatric-based protocol has better outcome than adult-based protocol. In this article, the protocol of ALL in children and adults are compared . It seems the first and probably the most important reason is the dose intensity of the nonmyelosuppressive chemotherapeutic agents, especially asparaginase.

Stromal cells are the important part of the bone marrow microenvironment. They provide a suitable environment for the self-renewal, proliferation and differentiation of the hematopoietic stem cells. Stromal cells include dendritic cells, macrophages, fibroblasts, endothelial cells and adipocytes. They secrete cytokines,extracellular matrix and provide direct cellular contaction to regulate hematopoiesis. Dysfunction of the stromal cells will lead to all kinds of hemopathy and immune system diseases directly.

The CD28-B7 are members of immunoglobulin superfamily.When B7 is engated by the CD28 ligand, a costimulatory signal occurs and transfers to T cell and it is important in the activation, proliferation, anti-apoptosis and promoting cytokine secretion of T cell.The CD28-B7 family is associated with tumor and autoimmune disease.