Objective To investigate the turnover and factors influencing amniotic membrane transplantation into corneal stroma.Methods Amniotic membrane stained with dichlorotriazinyl aminofluoresclin (DTAF)was implanted into the corneal stroma of 1 5 New Zealand white rabbits.After transplantation,we observed the clinical changes.The tissue samples from grafted area were observed with HE staining and laser confocal microscope to trace amniotic membrane at 4,8,13,1 7 and 22 week after surgery.And the tissue samples at week 22 after surgery from grafted area were also observed by transmission electron microscopy.Results After 4 weeks of operation, amniotic membrane tissue bent with visible multi-layer homogeneous red dye.The structure remained intact;basement membrane and stroma were no dissolved.After 8 weeks, amniotic tissue was a strip structure of homogeneous red dye,and the substance layer was complete.After 13 weeks,amniotic membrane morphology appeared fuzzy,but still took the shape of strips;a small number of corneal cells accumulated around the amniotic membrane.At 1 7 weeks after operation,fragmented amnion was evident.At 22 weeks after operation,small pieces of amnion tissue remained in the corneal stroma.Laser scanning confocal microscopy revealed that amniotic membrane could retain for over 22 weeks in the corneal stroma.After 22 weeks,amnion stroma collagen fibers that were integrated into the corneal stroma were irregular.Corneal stromal ultrastructure surrounding the amniotic membrane did not obviously change.Conclusion Retention time of amniotic membrane transplanted into corneal stroma can be as long as over 22 weeks and amniotic membrane can be merged into the corneal tissue as a substitute, which wins time for corneal transplantation operation.

Objective To observe the application of oxycodone with the efficacy and safety of propofol anesthesia in hysteroscopic surgery. Methods 60 cases of hysteroscopic surgery in our hospital in January 2016 January 2017 were admitted during the period, according to the anesthesia were divided into study group and control group, 30 cases in each. The study group used oxycodone with propofol the control group was treated with fentanyl anesthesia with propofol anesthesia effect and safety of anesthesia. Results Before anesthesia, study group and control group, heart rate and arterial pressure comparison, no statistically significant; after anesthesia, study group and control group, heart rate and arterial pressure contrast, comparison was statistically significant. The study group and the control group of adverse reactions during and after the operation, there was statistical significance. Compared with the control group, the analgesic effect of uterine contractions after 5min, 1 h and 2 h in the contrast group was statistically significant. Conclusion The application of oxycodone analgesia with propofol in the operation is effective in hysteroscopic surgery anesthesia, intraoperative respiratory depression, little effect on the circulatory system, high safety.

Bronchoalveolar lavage ( BAL) has been widely used in pediatric respiratory system disea-ses,and it has been comfirmed the value of the diagnosis and treatment in lung diseases. Collected bronchoalveo-lar lavage fluid ( BALF) is often used in microbiological pathogen identification,cellular and non-cellular com-ponents analyses. And it can be also used as guideline of clinical medication,diagnosis,treatment and pathogene-sis research of the respiratory disorders. This article summarizes the advance of BAL in pediatrics ,such as BAL operating skills,specimen transport and handling,and clinical application in lower respiratory tract infection and interstitial lung disease.

D-D4FC (?-D-2′,3′-didehydro-2′,3′-dideoxy-5-fluorocytidine),a new anti-HIV drug,is on its PhaseⅡ clinical trials in America,France and Germany. Our lab has synthesized D-D4FC successfully using N-deoxyribosyltransferase from Lactobacillus helveticto catalyzing the ribose transfer from D4T (?-D-2′,3′-unsaturated thymidine) to 5-FC (5-fluorocytidine).The yield of D-D4FC reached 25%.We discovered the reaction could also be done by using intact cells.The yield could increase to 50% in 12.5 hours and more convenient to industrial continuous process.In this paper,the conditions including pH,buffer,substrates concentration,cells amount,reaction time and a possible catalytic mechanism were studied and discussed.

Actins ; metabolism ; Angiomyolipoma ; pathology ; Carcinoma, Renal Cell ; pathology ; Diagnosis, Differential ; Epithelioid Cells ; metabolism ; pathology ; Female ; Gastrointestinal Neoplasms ; pathology ; Gastrointestinal Stromal Tumors ; pathology ; Humans ; Kidney Neoplasms ; metabolism ; pathology ; Lung Neoplasms ; pathology ; Lymphangioleiomyomatosis ; pathology ; Male ; Melanoma ; pathology ; Melanoma-Specific Antigens ; metabolism ; Pancreatic Neoplasms ; pathology ; Perivascular Epithelioid Cell Neoplasms ; metabolism ; pathology ; Sarcoma, Clear Cell ; pathology ; Skin Neoplasms ; pathology ; Uterine Neoplasms ; pathology

Humans ; Male ; Prostatic Hyperplasia ; complications ; Quality of Life ; Urinary Bladder, Overactive ; complications

Objective: Under the background of pension combined with medical service, for the elders to reasonably, effectively and fully utilize the medical resources, it is of vital significance to establish good medical con-sumptive behavior and consumption intention, to promote healthy old age and improve elders' health and happiness to help them cultivate a fine habit in their practical life. Methods: Based on the micro-survey data of investigation in Nanjing, this paper systematically analyzed the factors influencing elders' medical consumption behavior by using the theory of planned behavior and structural equation model. Results:Different factors were classified into behavioral at-titudes, subjective norms and perceived behavior control, and significant variables were found out through behavior model as well as though measuring their relative effects. Conclusion: There is no significant casual relationship be-tween the perceived behavior control and medical consumption behavioral intention. The elders' medical consumption behavior intention is affected by various social network relationships. The belief strength, normative belief, behavioral attitudes and subject norms can affect elders' medical consumption behavior.

OBJECTIVE:To develop a method for determining the entrapment efficiency of tetracaine hydrochloride liposome.METHODS:The microcolumn centrifugation was adopted to separate the free tetracaine hydrochloride from liposomes;the content of tetracaine hydrochloride was determined by HPLC and the entrapment efficiency was computed.RESULTS:By microcolumn centrifugation,the liposomes and the free tetracaine hydrochloride were well-separated.The recovery rate of the blank liposomes was 90.4%～100.1%;the absorption rate of free tetracaine hydrochloride was 96.6%～99.2%;and the entrapment efficiencies of tetracaine liposomes stood at about 80%.CONCLUSION:This method established for determining the entrapment efficiency of tetracaine hydrochloride liposome is simple,rapid and reproducible.

Objective To observe the expression of fractalkine (FKN or CX3CL1) in serum and lung tissue in early phases after paraquat (PQ) poisoning in rats,and to analyze the effect of FKN on acute lung injury induced by PQ.Methods A total of 66 SD rats were randomly (random number) divided into two groups,namely PQ group (n =36) and control group (n =30).Through intra-peritoneal route,PQ (22 mg/kg) was administered to PQ group,and an equal volume of normal saline to control group instead.Rats were separately sacrificed at 6 h,12 h,24 h,72 h and 120 h after poisoning.Lung coefficient was determined.The levels of FKN in serum and lung tissue homogenate were detected by ELISA.Lung pathological changes were observed by HE staining.FKN changes were investigated by immunohistochemistry staining.Data was analyzed by SPSS 19.0.Results From 6 h to 120 h after poisoning,parameter determined in PQ group had great changes,compared with the control group.At 6 h,12 h,24 h,72 h and 120 h,lung coefficients in PQ group were 5.03 ±0.07,5.17 ±0.10,5.46 ±0.10,5.68 ±0.15 and 5.83 ±0.11,respectively,which were significantly higher than those (4.49 ± 0.20,4.28 ± 0.13,4.45 ± 0.17,4.31 ±0.19 and 4.31 ±0.16) in control group (P＜0.01).Levels of FKN (pg/mL) in serum were 140.9 ± 15.8,157.9 ± 17.6,188.8 ± 24.8,224.4 ± 18.1 and 229.9 ± 10.0,respectively,significantly higher than those (121.7 ± 12.8,121.6 ± 12.1,118.3 ± 14.0,122.8 ± 12.4 and 120.5 ± 8.8) in control group (6 h P ＜0.05,others P ＜0.01).Levels of FKN (pg/mL) in lung tissue homogenate were 4 222.0 ± 641.1,5 021.0 ± 514.5,5 911.6 ± 478.1,7 092.2 ± 652.9 and 7 639.3 ± 666.6,respectively,significantly higher than those (2 860.2 ± 477.3,3 068.9 ± 446.0,3 168.7 ± 728.5,3 178.0 ±488.2 and 3 147.3 ±426.6) in control group (P ＜0.01).In PQ group,pathological changes manifested themselves in inflammatory cell infiltration,congestion,edema,structural damage,etc.The lung injury aggravated gradually from 6 h to 120 h.In control group,there was no significant change.FKN expressed mainly in bronchial cells,alveolar epithelial cells and pulmonary artery endothelial cells.Where there was higher expression of FKN,there were more inflammatory cells infiltrated.The level of FKN in lung tissue homogenate was positively correlated with lung coefficient (r =0.937).The level of FKN in serum was positively related to that in lung tissue homogenate (r =0.968).Conclusion There is correlation between FKN and acute lung injury induced by PQ in rats.

BACKGROUND:There is a close connection between the occurrence and development of tumor stem cels and ovarian cancer. CD90+ is an important tumor stem cel marker.
OBJECTIVE: To explore the biological characteristics of CD90+ tumor stem cels in ovarian cancer cels.
METHODS:The CD133 and CD90 positive rate of SKOV3 and primary ovarian cancer cels were detected by flow cytometry. The CD90+ and CD90- relative expression in stem cels and epithelium was detected by RT-PCR. Transwel invasion assay was employed to observe the cel invasion ability, clone formation test was done to observe cel proliferation and differentiation capacity, suspension bal test was adopted to observe pluripotent stem cels. The tumor formation time and tumor formation rate were observed by limited tumor dilution in immunodeficient mice.
RESULTS AND CONCLUSION:The positive rates of CD133 and CD90 in SKOV3 were significantly lower than those in primary ovarian cancer cels. The expression of CD133 and OCT4 in CD90+cels of SKOVS was significantly higher than that in CD90-cels of SKOVS. The expression of CD44, CD133, acetaldehyde dehydrogenase-1 and OCT4 in CD90+stem cels of primary ovarian cancer cels was significantly higher than that in CD90-stem cels of primary ovarian cancer cels. There were significant differences in the epithelial-mesenchymal related gene expressions between CD90-and CD90+stem cels of SKOV3 and primary ovarian cancer cels. With the increase of inoculated cels, the tumor formation rate of CD90-and CD90+ cels was increased continuously, but the tumor formation time was decreased. The tumor rate of CD90-cels was lower than that of CD90+cels. The number of transmembrane cels, cel clones and suspended cel bals was significantly higher in the CD90+ stem cels than the CD90-stem cels. These findings indicate that in ovarian cancer cels, CD90+stem cels can highly express stem cel-related genes and epithelial-mesenchymal related genes, which have a higher invasion, proliferation and differentiation ability, as wel as tumorigenic and pluripotent ability.