1.Evaluation of therapeutic studies on chemotherapy and/or radiotherapy-induced oral mucositis published in Chinese journals by means of evidence-based medicine
Na LV ; Juan XIA ; Chunyang LI
Journal of Practical Stomatology 1996;0(02):-
Objective:To evaluate the quality and the effectiveness of therapeutic studies on chemotherapy and/or radiotherapy-induced oral mucositis published in Chinese journals by means of evidence-based medicine. Methods:Relevant papers were searched in Chinese Biological Medical Disc (CBMDisc)database and China National Knowledge Infrastructure(CNKI) database. Papers using randomized controlled trials (RCT) were identified and analyzed according to the international standards of evidence-based medicine. Results:There were totally 98 articles focusing on therapeutic studies of chemotherapy and/or radiotherapy-induced oral mucositis and only 22 of them met RCT criteria. Study design, diagnostic standards, therapeutic effects and adverse effects decribed in those 22 papers were fully evaluated by means of evidence-based medicine. Conclusions:Research quality of the analyzed papers could not meet clinical needs and Meta analysis could not been done due to their low quantity and poor quality. However, as far as the published articles concerned, GM-CSF showed a good short-term treatment effect.
2.Study on abnormal expression of transforming growth factor-β1 in maternal blood and placenta of patients with fetal growth restriction
Na LV ; Guichan WANG ; Shaojun LI
Journal of Chinese Physician 2011;13(7):938-940
Objective To find out the relationship between fetal growth restriction (FGR) and transforming growth factor -β1 (TGF-β1). Methods The levels of TGF-β1 in maternal serum and placental tissue of FGR were detected with using ELISA and immunohistochemistry technique, and it was compared with those of normal term pregnancy. Results The levels of TGF-β1 in maternal serum of FGR group were significantly higher than those of normal term pregnancy (76. 5 ± 33. 4 VS 47.6 ± 24. 2, t' = 4. 65, P <0. 05). As for the intensity of the immunohistochemistry signal, TGF-β1 in syncytiotrophoblastic cells of FGR was markedly higher than that of control group (81.82% vs 5.83%, P <0. 01). Conclusions The levels of TGF-β1 are closely related with FGR.
3.HUMAN TfR GENE EXPRESSION BEFORE AND AFTER IRON SUPPLEMENTATION IN WHITE BLOOD CELL DETECTED BY FLUORESCENCE QUANTITATIVE POLYMERASE CHAIN REACTION
Na LV ; Ling ZHANG ; Ning LIU
Acta Nutrimenta Sinica 2004;0(06):-
Objective:To quantitively detect TfR gene expression of human white blood cell before and after iron supplementation. Methods:Before and after iron supplementation,10 healthy women were phlebotomized separately,and the real-time fluorescence quantitative PCR method was used to analyze the expression difference of TfR gene in human white blood cell. Results:TfR gene expression decreased after iron supplementation in 10 women,more evidently in 7 cases,where the expression level was half less than before iron supplementation. Conclusion:TfR gene expression of human white blood cell could be quantitatively measured by FQ-PCR,and was decreased after iron supplementation in 10 women. It explained that the regulative mechanism of TfR mRNA expression by iron is also suitable to white blood cell.
4.Feasibility of informatization, supermarketization, automatization for medical equipment inventory management
Liang CUI ; Zheng WANG ; Jun LV ; Na WEI
Chinese Medical Equipment Journal 1989;0(03):-
For a large-scale hospital, management of medical equipment is very important for its economic benefit and development. This paper discusses the implementation of informatization, supermarketization and automatization for medical equipment inventory management with computer network technology.
5.Qualitative Identification of 4 Constituents in Qindan Granules by TLC
Juntao LEI ; Shijie LV ; Kuang REN ; Yanxia JIANG ; Na XU
China Pharmacy 1991;0(03):-
OBJECTIVE:To establish the quality standard for Qindan granules. METHODS: The constituents including Radix Scutellariae, Radix Astragali, Cortex Moutan and Fructus Gardeniae in Qindan granules were identified respectively by TLC. RESULTS: The test samples and their respective reference substances were alike in that the same color chromatographic spots were noted at the corresponding places. The color spectra of the samples were clear yet without interference of surrounding impurity. CONCLUSION: The established method has high specificity and can be used for the quality control of Qindan granule.
6.Experimental study of sympathetic nerve on atrioventricular conduction modulation in acute inferior myocardial infarction
Bin DONG ; Maorong CAO ; Furong LV ; Na WEN ; Hang CAI
Clinical Medicine of China 2008;24(9):891-893
Objective To study atrioventricular conduction during right coronary artery infarction and its correlation with sympathetic nerve.Method AIMI model by ligating the fight coronary artery of denervated autonomic nervous cats was used.The A,H and V-wave were measured from the His electrode by template matching.The AA,AH,HV intervals with and without sympathetic nerve stimulation were measured respectivelv.Results The decreased percentage of AH intervals was (7±5)% vs.(14±5)% (without pacing) and (12±2)% vs.(23±7)% (with pacing) respectively in ischemia and normal after sympathetic nerve stimulation,and the decreasing amplitude were 50% and 48% respectively.Conclusion The regulating function of sympathetic nerve on atrioventricular conduction decreased after ischemia related to the right coronary artery.This decrease may be one of the mechanisms that lead to atrioventficular block after acute inferior myocardial infarction.
7.Efficacy of Trimebutine Combined with Mosapride on Functional Dyspepsia
Yingbin HU ; Jueping FENG ; Na PENG ; Fei LV ; Qiuxia GUO
Herald of Medicine 2014;(7):887-890
Objective To evaluate the efficacy and safety of trimebutine combined with mosapride on functional dyspepsia. Methods Patients with functional dyspepsia were randomly divided into three clinical groups. Group A (n=116) received 0.2 g trimebutine after meal,group the drug combination B (n=116) received 5 mg mosapride before meal,and the drug combination group (n=115) received 0. 2 g trimebutine after meal plus 5 mg mosapride before meal. All medications were taken orally three times daily for 4 weeks. Improvement in clinical symptoms and adverse reactions in each group were evaluated at the end of study. Results A total of 339 patients among 347 enrollees completed the treatment and follow-up. The clinical efficacy on postprandial fullness, early satiation, epigastric pain, epigastric burning, upper abdominal bloating and nausea were 88. 4%,76. 9%,72. 9%,61. 8%,86. 7% and 81. 7%,respectively in the drug combination group after 4-week treatment,which were superior to those in group A or B (P<0. 05) except for epigastric burning. The total effective rate of the drug combination group was 78. 8%,significantly higher than the other two groups (P<0. 05). The total incidence of side effects in the drug combination group was 1. 8%,similar to that of group A and B (1. 8% and 0. 9%,respectively, P =0. 776). Conclusion Trimebutine combined with mosapride is safe and effective for improving symptoms in functional dyspepsia.
8.The signal pathway of Lin28 and tumor
Yunlu JIA ; Kezhen LV ; Na LIN ; Wenxian HU
Chinese Journal of Clinical Oncology 2014;(8):530-533
Lin28 is a highly conserved RNA-binding protein that has an important role in human body development, metabolism, and tumorigenesis. Previous studies have found that the activation of Lin28 can suppress the maturity of let-7 miRNA in stem cells and promote the proliferation of stem cells by upregulating cell-cycle related genes, such as cyclins A and B. Other mechanisms underlying the activation of Lin28 can selectively block the processes of pre-let-7 miRNA in embryonic stem cells and ultimately promote tumori-genesis. In addition, Lin28 affects the occurrence, development, and prognosis of cancer by inhibiting the differentiation and maturation of miRNA and by moderating the expression of some cell-cycle related genes. Lin28 has an important function in the tumor's tolerance of chemotherapy and radiotherapy. We review the physiological function of Lin28 in tumors and its function in tumor treatment.
9.Effects of Matrine in Combination with 3 Antitumor Drug on Cell Cycle of KBV200 Cell Line
Hongyan CHEN ; Yan CHEN ; Caifeng LV ; Xiaoyan YU ; Na SHEN ; Qianying ZHANG ; Ling YE
China Pharmacy 2001;0(07):-
OBJECTIVE:To observe the effects of matrine in conjunction with vincristine(VCR),adriamycin(ADM)and diamminedichloroplatinum(DDP),respectively on the cell cycle of the KBV200drug-resistant cell line.METHODS:The cultured KBV drug-resistant cells were divided into control group,antitumor drug groups and the combination therapy groups(marine+antitumor drugs,respectively),FCM assay was used to detect and analyze the expression of cell cycle and apoptosis of each group.RESULTS:As compared with single antitumor drugs groups,no significant change was found in the proportion of cells and cell apoptosis in the combination therapy groups(matrine+VCR or matrine+ADM).But marine+DDD resulted in a reduction of the G 0 ~G 1 stage cell population but an increase of G 2 ~M stage cell population.CONCLUSIONS:It was assumed that matrine-induced reversal of KBV200cells'drug-resistant to VCR and ADM may not relate to cell cycle and cell apoptosis.Matrine can slightly enhance the cytotoxic effect of DDP.
10.Isolation and identification of Toxoplasma gondii strains from cats in Xu-zhou region
Linlin FU ; Chao YAN ; Zhuanzhuan LIU ; Delong KONG ; Liang LV ; Na SHI
Chinese Journal of Schistosomiasis Control 2014;(6):656-657,668
Objective To isolate Toxoplasma gondii T. gondii strains from stray cats and explore their prevalence in Xu?zhou City. Methods The sera of 41 stray cats were collected to detect the antibodies of T. gondii by using a commercial enzyme?linked immunosorbent ELISA kit. The tissues including the heart brain and tongue from these cats were digested by acid pep?sin solution and inoculated to Kunming mice. These suspicious isolates were subsequently identified by a specific PCR method. Results A total of 11 strains were isolated from 41 stray cats which were confirmed by the PCR results. Moreover 17 cats 41.5% were found to be positive with the antibodies of T. gondii. Conclusion A high prevalence of T. gondii infection was found in Xuzhou City which indicates that the stray cats infected with T. gondii would be an important infection source that may infect humans and other animals in this area.