OBJECTIVE:To provide reference for specified labeling of medication information on pregnant women in drug in-structions of anti-diabetic drugs. METHODS:All of drug instructions of anti-diabetic drugs in DXY Drugs Information were collect-ed, the medication information labeling on pregnant women was analyzed. RESULTS:Among 166 drug instructions, 147 (88.55%)had listed the item ofdrug use for pregnant and lactating womenalone,and labeled with the drug information on preg-nant women;14(8.43%)had listed the item too,but labeled with no or limited clinical data,and whether pregnant women could use or not was not suggested;5(3.01%)didn't have the item. About without guidance of drug use,there were 3(1.81%)drug in-structions missed guidance,and 14(8.43%)with no or limited clinical data. About with guidance of drug use,49(29.52%)were labeled with forbidden for pregnant women;40(24.1%)were labeled with can't/not suitable/should not be used for pregnant wom-en;49 (29.52%) were labeled with cautious/not recommend/weighed the pros and cons/benefits greater than the risk/consulted a doctor for pregnant women;and 11(6.63%)were labeled with being safe/being not restricted for pregnant women. In the 166 drug instructions,26,which were from foreign pharmaceutical companies,had listed the item ofdrug use for pregnant and lactating womenalone,as well as labeled the guidance of drug use for pregnant women. CONCLUSIONS:The labeling of domestic drug instructions shows inadequate drug information on pregnant women,non-uniform guidance statements,lag of update and other prob-lems,which should be attached to great importance by drug supervision and management departments,as well as pharmaceutical production enterprises.

Ginsenosides (GS)as the main ingredient of ginseng,include dozens of monomer,such as Rg3,Rh2,Rd,Rh1 ,Rg2,etc.GS and various monomers have different anti-hepatoma mechanisms.The main mechanism includes the following aspects,inducing the apoptosis of liver cancer cells,inhibiting the prolifera-tion,invasion and metastasis,reversing the multidrug resistance of liver cancer cells,increasing therapeutic effects by combining with other treatments,improving the immune function of organism and inhibiting hepatic glu-cose production.Different GS monomers play different roles of anti-hepatoma through their respective channels.

Objective To investigate the levels of cystatin C (CC) with the H&Y scale and duration of Pakinson's disease(PD)patients.Methods The study consists of 432 PD patients and 513 control subjects.PD patients were divided into 3 subgroups according to their H&Y scale.The levels of CC and Scr were measured.The statistics analysis was used to evaluate their associations.Results In PD and control groups, CC concentrations were significantly different (P ＜ 0.05), and Scr concentrations in two study groups were not significantly different(P ＞ 0.05).PD in each subgroup, CC concentrations in the medium group were significantly higher than the early and control groups, and CC in the late group was significantly higher than the other two subgroups.Rank correlation analysis and comparison of CC concentration and duration and H&Y score showed that CC concentration was positively correlated with the duration of PD(r =0.209, P ＜0.01), and that CC concentration and H&Y score were significantly positively correlated (r =0.618, P ＜0.01).Conclusions There was a certain correlation between CC levels and PD, and CC levels of PD patients were significantly higher.There was no significant increased CC in the initial stages of the disease, perhaps CC was not necessary cause of the onset of PD.However, the level of CC with H&Y stage and duration were positively correlated, perhaps CC may be involved in the process of disease progression, because high level of CC may increase PD patient's condition.

Objective To explore the antigen presentation of CT26.WT via intra-peritoneal injection. Methods The intra-peritoneal injection model was made via injecting cell suspensions in mice. The spleen was isolated from BALB/c mice toco-culture with CT26.WT to detect tumor-killed ability. Phenotype identification methods and CCK8 massy were used to measure the ability of antigen presentation and stimulate T lymphocyte proliferation. IHC was used to detect the expression of B7H4 in normal and tumor tissues. Results Along with the extension of intra-peritoneal injection, the surviving number of cells was increased, contrary to the apoptosis. DC cells failed in maturation and impaired in stimulating T lymphocyte proliferation. B7H4 was higher in tumor tissues. Conclusions With the extension of intra-peritoneal injection, the mature DC cells were scared in number, resulting in the impairement of antigen-presentation. Moreover, the higher B7H4 expression in tumor tissues led to the lack of second signals which may stimulate T cells. Consequently, the ability of T cells in killing tumor cells was decreased so that they escape immunosurveillance.

Objective To observe the intervention effect of acupuncture on mammalian target of rapamycin(mTOR)of hypoxic-ischemic encephalopathy(HIE)model in newborn rats.Methods Seven-day-old SD rats were randomly devided into six groups.Sham-surgery group:the bilateral common carotid arteries were separated,without ligation,and the rats were without hypoxia.HIE model group:the bilateral common carotid arteries were separated and ligated,and the rats were in low oxygen.In the sham-acpuncture group,four positions were acupunctured:from Baihui,Dazhui,Quchi and Yongquan by 1 cm after hypoxia ischemia.In acpuncture group,four positions were acupunctured:Baihui,Dazhui,Quchi and Yongquan after hypoxia ischemia,positioned according to the ordinary acupoints for acupuncture in rats.In the antagonists group,the HIE model rats were injected wortmannin 5 μl to the left ventricle.The acupuncture and antagonists group had the same operations of antagonists group and acupuncture group at the same time.All the groups were subdivided into four subgroups in terms of four time periods of 1 d,3 d,7 d and 21 d.The morphological changes of organizational structures of hypoxic ischemia side in brain tissue were observed by pathological methods.The levels of mTOR mRNA expression were detected by RT-PCR.Results In acupuncture group, acupuncture treatment for 21 d showed that acupuncture group had clearly organizational structure,regular arrangements of neurons,normal cell outline,relatively clear nucleolus,mitigated swell neuron and hyperplas-tic glial cells.Compared with that of the HIE model group,acupuncture treatment increased mTOR mRNA dramatically on day 1 ,day 3,day 7 and day 21 (Q =7.099 1 ,9.009 9,7.894 0,9.914 7,P ﹤0.05 respective-ly).The expression of mTOR mRNA in acupuncture and antagonists group was significantly higher than that in sham-surgery group on day 1 ,day 3 day 7 and day 21 (Q =9.868 9,15.843 4,21.007 2,P ﹤0.05 respec-tively).In day 21 ,compared with the sham operation group and the HIE model group,the expression of mTOR mRNA in acupuncture and antagonists group were significantly higher (Q =18.236 0,4.729 7, P ﹤0.05 respectively).Conclusion Electroacupuncture treatment can promote the expression of mTOR at the mRNA level and protect brain of newborn rats with HIE.

Objective To introduce a simple fixing method for tail vein injection in mice.Methods Twenty tumor-bearing male BALB/c nude mice were used in this study.Tail vein injection was performed to these mice by two laboratory technicians A and B, respectively.The injection time and success rate were recorded and analyzed.Results Mouse tail vein injection was successfully completed by the two technicians with the cage lid pressing method.Conclusions Cage lid pressing method is a simple method for tail vein injection in mice, especially provides a more efficient method for those special form of mice.

International Cooperation ; Nephrology ; trends ; Pediatrics ; trends

Objective To construct for the heredity linkage map and to study the functional gene research of Carthamus tinctorius,the factors affecting cDNA amplified fragment length polymorphism(cDNA-AFLP) of C.tinctorius were investigated with developing and optimizing the cDNA-AFLP reaction system.Methods Improved Trizol method was used to extract RNA from compounds in new petals specific to safflower.With the help of M-MLV RTase without RNase activity combined with replacement synthesis method,double-stranded cDNA was synthesized from total RNA;cDNA was digested by restriction enzyme MseI/EcoRI and ligated by two steps.Then the products were provided for pre-amplification and selected amplification of different concentration gradients.After tiny modifications of system concentration,finally PAGE electrophoresis and silver-staining were performed.Results High purity and integrated total RNA for later cDNA synthesis were obtained and high quality cDNA was synthesized with the help of M-MLV RTase without RNase activity combined with replacement synthesis method.The cDNA-AFLP reaction system in C.tinctorius was as follows: 250 ng integrity cDNA was digested thoroughly at 37 ℃ for 6 h,and ligated 12 h at 16 ℃.Furthermore,the sample dilution multiplication was 10 fold for pre-amplification and 150 fold for selected amplification under the proper system concentration.According to the above reaction system,the polymorphous strips with high resolution power in PAGE electrophoresis were clear and stable.Conclusion The cDNA-AFLP reaction system established and optimized in this experiment is suitable for the functional gene analysis of C.tinctorius.

Objective: To study the role of occupational stress in CHD (coronary heart disease) Methods: 39 male attendants on train with CHD were identified through Rose questionnaie and were confirmed after one year Two control groups were collected according to the rate of 1:2 and matched by sex, age, job, year of working separately in railway system (n=78) and hotel service (n=78) All subjects completed OSI (occupational stress indicator) Logistic regression model was used to analyze the data Results: Exceptfor non-occupational stress, the relative risk of train attendant working was estimated as OR=2 18 with 95% confidence interval of 1 15～4 15, and the risk for CHD as a train attendant had dose-effect relation to occupational stress Compared with controls of railway system without CHD, the relative risk of stress was still significant (OR=4 43, 95% CI: 1 60～12 25) But the relative risk (OR=9 75, 95%CI:3 18～29 92) was much higher when comparing with controls of hotel service Conclusion: Occupational stress is one of the important risk factors for CHD in male attendants on train

Adenoma, Pleomorphic ; surgery ; Adult ; Female ; Humans ; Male ; Middle Aged ; Nasopharyngeal Neoplasms ; surgery