Sepsis is systemic inflammatory response syndrome by infection.It always leads to sepsis shock and multiple organ dysfunction syndrome.Sepsis is the most common cause of children death,it is the hotspot and difficulty of the research field on the modem children's critical care medicine.Acute respiratory distress syndrome,is the significant cause of deterioration and death.This paper maked a review on the pathogenesis of acute respiratory distress syndrome induced by sepsis and the treatment function of Ulinastafin.

Objective: To investigate the efficacy of the green tortoise exploring point technique for treating piriformis syndrome. Methods: Ninety patients were randomly allocated to a green tortoise exploring point treatment group of 30 cases, an acupuncture control group of 30 cases and a hydro-acupuncture control group of 30 cases for clinical observation. Results: The symptoms improved after treatment in all the three groups. The total efficacy rate was 100.0% in the treatment group, 76.7% in the acupuncture group and 83.3% in the hydro-acupuncture group. There were statistically significant differences between the three groups (P<0.01, P<0.05). Conclusion: The technique of green tortoise exploring point is better than conventional acupuncture and hydro-acupuncture in treating piriformis injury syndrome

Objective To investigate the distribution and antibiotic resistance of clinical pathogen isolated from patients of intra-abdominal infection associated sepsis in surgical intensive care unit (SICU ) during recent 5 years ,then instruct clinical application of antibiotics reasonably .Methods Abdominal drainage of 65 patients of intra-abdominal infection associated sepsis were collected from January 2008 to December 2012 .All the specimens were analyzed for pathogen distribution and drug susceptibility .Results A total of 151 strains of pathogenic bacteria was isolated ,in which Gram-negative bacteria were the most popular pathogen (104 strains ,68 .9% ) ,whereas Gram-positive bacteria and fungi accounted for 19 .2% (29 strains) and 11 .9% (18 strains) ,respectively . The five most common pathogens were Escherichia coli(23 .2% ) ,acinetobacter baumannii(15 .2% ) ,enterococcus faecium(13 .2% ) , pseudomonas aeruginosa(8 .6% ) ,andklebsiella pneumonia(7 .9% ) .The rate of the ESBL-producing strains of escherichia coli and pneumonia pneumonia were 80 .0% and 33 .3% .Both of them showed low resistance to carbapenem antibiotics .The proportion of multidrug resistantstrains and extensive drug resistant strains of acinetobacter baumannii was 56 .5% and 30 .4% ,respectively .Con-clusion The resistance of the pathogen isolated from the patients of intra-abdominal infection associated sepsis in SICU is seriously high .Early and rational using of antibiotics is of great importance to control the production and transmission of drug resistance .

Objective To explore the antigen presentation of CT26.WT via intra-peritoneal injection. Methods The intra-peritoneal injection model was made via injecting cell suspensions in mice. The spleen was isolated from BALB/c mice toco-culture with CT26.WT to detect tumor-killed ability. Phenotype identification methods and CCK8 massy were used to measure the ability of antigen presentation and stimulate T lymphocyte proliferation. IHC was used to detect the expression of B7H4 in normal and tumor tissues. Results Along with the extension of intra-peritoneal injection, the surviving number of cells was increased, contrary to the apoptosis. DC cells failed in maturation and impaired in stimulating T lymphocyte proliferation. B7H4 was higher in tumor tissues. Conclusions With the extension of intra-peritoneal injection, the mature DC cells were scared in number, resulting in the impairement of antigen-presentation. Moreover, the higher B7H4 expression in tumor tissues led to the lack of second signals which may stimulate T cells. Consequently, the ability of T cells in killing tumor cells was decreased so that they escape immunosurveillance.

Age-related macular in human retinal results in strict loss and enduring decrease in visual acuity. A lot of medical evidence has shown that macular degeneration, associated with Choroidal neovascularization (CNV), leads to irreversible injure in composition and function of retinal tissue. A new analytical and restorative treatment, scotoma-based photocoagulation (SPB), curdles the affected areas so effectively and timely that it can delay or prevent age-related macular degeneration (AMD). A scanning laser ophthalmoscope (SLO) image is involved and the function and character of scotometry are analyzed. Combining anatomical and pathological features, this paper introduces process of acquiring the image and algorithms of analyzing it. Furthermore, integrate image of retinal is extracted from SLO through feature extraction, special registration and superimposition. The treatment can be applied to clinical diagnosis without great revisions and provide direction for pertinent fields.

Objective: Under the background of pension combined with medical service, for the elders to reasonably, effectively and fully utilize the medical resources, it is of vital significance to establish good medical con-sumptive behavior and consumption intention, to promote healthy old age and improve elders' health and happiness to help them cultivate a fine habit in their practical life. Methods: Based on the micro-survey data of investigation in Nanjing, this paper systematically analyzed the factors influencing elders' medical consumption behavior by using the theory of planned behavior and structural equation model. Results:Different factors were classified into behavioral at-titudes, subjective norms and perceived behavior control, and significant variables were found out through behavior model as well as though measuring their relative effects. Conclusion: There is no significant casual relationship be-tween the perceived behavior control and medical consumption behavioral intention. The elders' medical consumption behavior intention is affected by various social network relationships. The belief strength, normative belief, behavioral attitudes and subject norms can affect elders' medical consumption behavior.

OBJECTIVE:To develop a method for determining the entrapment efficiency of tetracaine hydrochloride liposome.METHODS:The microcolumn centrifugation was adopted to separate the free tetracaine hydrochloride from liposomes;the content of tetracaine hydrochloride was determined by HPLC and the entrapment efficiency was computed.RESULTS:By microcolumn centrifugation,the liposomes and the free tetracaine hydrochloride were well-separated.The recovery rate of the blank liposomes was 90.4%～100.1%;the absorption rate of free tetracaine hydrochloride was 96.6%～99.2%;and the entrapment efficiencies of tetracaine liposomes stood at about 80%.CONCLUSION:This method established for determining the entrapment efficiency of tetracaine hydrochloride liposome is simple,rapid and reproducible.

Objective To assess the impact of different number of diffusion gradient directions (NDGD) of diffusion tensor imaging (DTI) on dispersion degree of fractional anisotropy (FA) values and signal-to-noise ratio (SNR) for adult brain tissues. Methods Eight healthy volunteers underwent DTI with 7 NDGD respectively, and the associated FA maps were obtained. Four region of interest (ROI) (genu and splenial of corpus callosum, genu and posterior limb of internal capsule) were chosen in white matter of FA maps, FA values and the corresponding SNRs of the ROIs were computed and analyzed with NDGD increasing. Results FA values within all ROIs did not change remarkably with NDGD increasing. Variance of FA value within two ROIs with high gray value (genu and splenial of corpus callosum) fluctuated randomly and SNR increased slightly, while variance of FA value within the ROIs with low gray value (genu and posterior limb of internal capsule) diminished significantly with NDGD increasing. Conclusion In clinical applications of DTI, an optimum NDGD for DTI data acquisition should be selected according to the ROIs in human brain to be inspected.

Child ; Congresses as Topic ; Humans ; Pediatrics ; Sleep

Objective To investigate the effect of N-methyl-N-nitrosourea (MNU) on expressions of interphotoreceptor retinoid-binding protein (IRBP) and recoverin in rat retinal.Methods Thirty female Sprague-Dawley (SD) rats were randomly divided into five groups,including normal control group,and MNU treatment one day,three days,seven days and 10 days groups,each group had six rats.Rats in MNU-treatment groups received a single intraperitoneal injection of 40 mg /kg MNU,while rats in the normal control group received intraperitoneal injection of saline 5 ml/kg.Reverse transcription-polymerase chain reaction (RT-PCR) and immunofluorescence were used to detect the retinal expression of IRBP and recoverin.Results RT-PCR results indicated that retinal IRBP levels decreased after MNU injection compared with normal control (P＜0.01),while recoverin levels increased (one day:P＞0.05; three days:P＜0.05; seven days:P＜0.5; 10 days:P＜0.05).Immunofluorescence assays demonstrated that normally IRBP protein is mainly in the inner and outer segments of photoreceptors.After MNU treatment,IRBP protein was detected in all retinal layers but much faint.Recoverin was expressed in the inner nuclear layer,inner plexiform layer and ganglion cell layer,and its expression was increased after MNU injection.Conclusion MNU suppresses IRBP expression and promotes recoverin expression in rat retina.