Objective To investigate the health-related demands in main caregivers for patients with Parkinson's disease after surgery. Methods From March to December, 2014, twelve main caregivers were investigated with a semi-structured interview, and analyzed with Co-laizzi's analysis. Results and Conclusion Four aspects of demand were found, including knowledge about the disease, rehabilitation nursing skills, economic support and family support. Medical professionals can provide the targeted knowledge and technical guidance to meet their demands.

ObjectiveTo observe the clinical efficacy of acupuncture-moxibustion in treating tinnitus.MethodNinety-eight patients with tinnitus were divided into a warm-needling group (n=32), an acupuncture group (n=34), and a medication group (n=32), to observe thetherapeuticefficacies.ResultThe total effective rate was 83.3% in the warm-needling group, versus 78.8% in the acupuncture group and 60.0% in the medication group.ConclusionWarm needling and acupuncture plus TDP are both superior to the medication treatment in comparing the therapeutic efficacy.

Objective To verify that natural zeolites can be used in treatment of fluoride in groundwater in the east-southern of Shanxi Province. Methods Determination of the fluoride by lime-paper sampling and fluorine ion-selective electrode analysis(reference to GB/T 7484-1987). In the paper the best activation process and the appropriate application were discussed, and then the activated zeolite should be used in experimental and real application, in which the effect of zeolite on water quality were assured. The used zeolite could be regenerated and reused. Results The best activation process before use was orderly treated by 5.0 mol/L HCl for 5 hours, then 0.3 mol/L KAl(SO4)2 10 hours,and finally 300 ℃ in furnace for 4 hours. 5 g activated zeolite in 100 ml water(solid∶liquid =1∶20). The static analysis insisted that activated zeolite had a significant adsorption for fluorine. The dynamic analysis verified that the fluoride level could reach the standard after 40 min of treatment. Regenerated with 2.0% KAl(SO4)2,the effect of removing fluoride was 45.0%. Conclusion The experiments testified that the processed natural zeolite can used in water treatment of high fluoride groundwater.

Objective To investigate the role of subtype 1 autoantibody against angiotensin Ⅱ receptor in the pathogenesis of preeclampsia by detecting its expression in the peripheral blood of preeclampsia patients,Methods Thirty patients with preeclampsia were assigned to preeclampsia group. Twenty normal pregnant women at the late stage and twenty non-pregnant healthy women as controls were investigated. The level of type 1 antoantibody against angiotensin Ⅱ in the peripheral blood was detected by indirect SA-ELISA assay with the produced ATR-1 as the antigen. Results The level of subtype 1 antoantibody against angiotensin Ⅱ (65 ±4. 7) % in the peripheral blood of preeclampsia patients is significantly higher than that of normal late pregnant (26 ±2. 8)% and non-pregnant women(7.8 ±2. 2)% groups (t1 =24. 97 ;t2 =38.56;P ＜0. 01 for both) ;The angiotensin Ⅱ receptor subtype 1 autoantibody in the group of normal late pregnancy (26 ± 2. 8 )% was significantly higher than that of healthy non-pregnant women group ( 7. 8 ± 2. 2 ) % ( t = 4. 58, P ＜ 0. 05 ).Conclusion Compared with the normal pregnant women and the healthy non-pregant women, the autoantibody against AT1 receptor in sera of preeclamptic patients is elevated ata high frequency. These results suggest that overproduction of AT1-AA may play an important role during the development of preeclamptic patients. AT1-AA is a novel risk factor in pregnant women. Immune mechanisms may be involved in the process of pregnancy.

OBJECTIVE To discuss the practicality of using the nylon nets,which are derived from disposed used medical(suture-band).The nylon nets were attached to the filtering apparatus on original return-wind exit on the lateral wall of surgical operating room.METHODS The velocity of wind,concentration of bacteria and the total(particle) amount of dust were determined by contrasting the number determined before and after passing the nylon nets on the(filtering) apparatus return-wind exit.RESULTS There was no evidence to influence the cleaning and temperature(regulating) effects of air conditioner by binding the nylon nets on them.CONCLUSIONS This method appears to save time as well as energy to work more efficiently than cleaning air conditioners weekly.

Age-related macular in human retinal results in strict loss and enduring decrease in visual acuity. A lot of medical evidence has shown that macular degeneration, associated with Choroidal neovascularization (CNV), leads to irreversible injure in composition and function of retinal tissue. A new analytical and restorative treatment, scotoma-based photocoagulation (SPB), curdles the affected areas so effectively and timely that it can delay or prevent age-related macular degeneration (AMD). A scanning laser ophthalmoscope (SLO) image is involved and the function and character of scotometry are analyzed. Combining anatomical and pathological features, this paper introduces process of acquiring the image and algorithms of analyzing it. Furthermore, integrate image of retinal is extracted from SLO through feature extraction, special registration and superimposition. The treatment can be applied to clinical diagnosis without great revisions and provide direction for pertinent fields.

Objective: To explore the effect of atorvastatin (Atv) on high glucose-induced oxidative stress injury in human umbilical vein endothelial cells (HUVECs) by SIRT1/NADPH oxidase pathway with the possible mechanisms.
Methods: HUVECs were cultured in low glucose medium and then divided into 6 experimental groups:①Normal group,②Osmotic pressure control group,③High glucose (HG) group,④HG+Atv (0.1, 1.0, 10.0)μmol/L group,⑤HG+sirtinol (SIRT1 inhibitor) group,⑥HG+apocynin (NOX4 inhibitor) group, and HUVECs were further cultured for 24 hours. The cell proliferation was examined by CCK-8 kit, ROS level was detected by lfow cytometry method, protein expressions of SIRT1 and NOX4 were measured by Western blot analysis.
Results: ① Compared with Normal group, HG group had decreased HUVECs proliferation, Atv improved the HG inhibited proliferation in a does dependent manner. ② HG group had the higher level of ROS, increased NOX4 protein expression and decreased SIRT1 protein expression. ③ In HG condition, Atv up-regulated SIRT1 expression and down-regulated ROS and NOX4 expressions in a does dependent manner.④In HG condition, sirtinol decreased SIRT1 expression, increased NOX4 expression, and apocynin decreased NOX4 expression, while it had no inlfuence on SIRT1 expression.
Conclusion: Atorvastatin could resist HG-induced oxidative stress injury in HUVECs, which might be related to up-regulated SIRT1 expression, and SIRTI plays the role in NADPH oxidase at upstream.

BACKGROUND:Osteoporosis caused by diabetes melitus as common secondary osteoporosis has been paid more and more attention recently. Zoledronic acid serves as a novel drug for osteoporosis, and its effect on osteoblasts in vivo remains unclear. OBJECTIVE:To investigate the changes of the expression of bone morphogenetic protein 2 andNoggin in the femur of type 1 diabetes melitus rats and the effect of zoledronic acid on them. METHODS:Models of type 1 diabetes melitus were established by intraperitoneal injection of streptozotocin in 130 Wistar rats. 3 days later, rats with blood sugar > 16.7 mmol/L for three consecutive times were considered as successful models, 120 in total. These models were randomly divided into model, prevention and treatment groups. Rats in the prevention and treatment groups were intravenously administered zoledronic acid (0.1 mg/kg) on the day of modeling and 2 weeks after model establishment. An additional 40 rats were injected with citrate buffer solution as control group. RESULTS AND CONCLUSION: Compared with the control group, femur bone mineral density, serum alkaline phosphatase levels, and femur bone morphogenetic protein 2 mRNA expression levels were significantly lower in the model group (P < 0.05), butNoggin mRNA expression significantly increased (P < 0.05). Compared with the model group, bone mineral density and bone morphogenetic protein 2 mRNA expression levels were significantly higher in the prevention and treatment groups (P < 0.05), butNoggin mRNA expression significantly lower (P < 0.05), and serum alkaline phosphatase levels gradualy restored. These results indicated that the bone metabolic disturbance occurs in early stage in rats with type 1 diabetes melitus. Zoledronic acid can promote bone formation, increase bone density, and improve bone metabolism.

BACKGROUND:In previous experiments, TiO2-xNy OBJECTIVE:To study the friction properties of orthodontic brackets coated with TiO-coated bracket has been confirmed to have excelent antibacterial properties and biological safety performance. 2-xNy METHODS: TiO film. 2-xNy film was prepared by radiofrequency magnetron sputtering on MBT bracket (0.022″). The TiO 2-x N y RESULTS AND CONCLUSION: TiO-coated brackets were analyzed by X-ray diffraction and scanning electron microscopy observations. The coefficient of static friction and coefficient of kinetic friction between the wires (0.012″, 0.014″, 0.016″) and orthodontic brackets were calculated. 2-xNy film on the bracket was of anatase structure, which was compact and had good crystalinity. Under dry condition, the coefficient of static friction and kinetic friction of the brackets coated with TiO2-xNy were less than those of ordinary brackets; under wet condition, the coefficients of static friction and kinetic friction of the brackets coated with TiO2-xNy were less than those of ordinary brackets, but the difference was not statisticaly significant. Nano-TiO2-xNy film can reduce the friction between bracket and archwire, which wil offer a novel opportunity to significantly reduce the friction during tooth movement.

Objective:To explore the functions of cytokines and TM in the pathogenesis of acute cerebral infarction.Methods:The levels of IL-1、IL-6 、TNF and TM were detected by ELISA in 55 patients with acute cerebral infarction.Results:The levels of IL-1、IL-6 、TNF and TM were increased significantly in the patients with acute cerebral infarction in comparision with the controls ( P