The impact of the pandemic, caused by SARS-CoV-2, on the management of malignancies has posed several challenges. Gestational trophoblastic diseases, being considered highly curative, are categorized as emergent cases which need immediate intervention with a level I priority for chemotherapy for its malignant spectrum. These guidelines have been drawn up to assist in decision-making and the implementation of such are dependent on the capabilities of each institution combined with their local experience.

Objective The physiological and biochemical changes were studied during the Panax notoginseng′s fruit development.Methods Dynamic changes of fruits size,fresh and dry weight,moisture content and soluble sugar,starch and protein contents were investigated.Results The moisture content was increased first and then dropped to 73.37% at maturity.The fresh and dry weight,starch and protein contents of P.notoginseng′s fruits were increased with the fruit development.The soluble sugar content was raised first,then decreased,and increased slightly at the lateral stage of fruit development.Conclusion The P.notoginseng′s fruits are mature at 80 d after peak anthesis;the decrease of moisture content is companied with the gradual increasing of fruit fresh and dry weight during the fruit development.The storage and utilization of nutrients in P.notoginseng′s fruits are closely related with the fruit development.

Objective To develop and verify a plaque method for detection of infectious titer of tick-borne encephalitis virus(TBEV)strain(PHKT strain for short)adapted to primary hamster kidney(PHK)cells.Methods PHK cells were infected with TBEV,a primary mouse brain adaption strain,and passed consecutively for 12 passages.The titer of PHKT was detected by plaque method(Monolayer BHK-21 cells were infected with PHKT of various passages at different dilution ratios,and the plaque number was calculated by neutral red staining)and challenge titration in mouse brain(Mice were challenged with PHKT of various passages at different dilution ratios through brain cavity,0.03 mL for each,observed continuously for 14 days,and calculated for the median lethal dose(LD50)by Reed-Muench method)respectively,and the correlation between the results of two methods was analyzed.The developed plaque method for the detection of TBEV titer was verified for specificity,repeatability and intermediate precision.Results The plaque titer of PHKT virus was up to8.9 lgPFU/mL;The correlation between the results of plaque method and mouse brain challenge titration method was good(r = 0.92);The specificity of plaque method for detecting infectious titer of PHKT virus was good,and the coefficients of variation(CVs)of repeatability and intermediate precision were both less than 5%.Conclusion A plaque method for detecting infectious titer of PHKT virus was developed,which may be used as an alternative method for challenge titration in mouse brain.

Complement System Proteins ; metabolism ; Hemolytic-Uremic Syndrome ; diagnosis ; therapy ; Humans

Alkyl and Aryl Transferases ; genetics ; Child ; DNA, Mitochondrial ; genetics ; Fibroblasts ; metabolism ; Glomerulosclerosis, Focal Segmental ; diagnosis ; drug therapy ; genetics ; Humans ; Kidney Diseases ; diagnosis ; drug therapy ; genetics ; Mitochondrial Diseases ; diagnosis ; drug therapy ; genetics ; Mutation ; Nephrotic Syndrome ; diagnosis ; drug therapy ; genetics ; Protein Kinases ; genetics ; Ubiquinone ; analogs & derivatives ; biosynthesis ; deficiency ; therapeutic use

Sepsis is systemic inflammatory response syndrome by infection.It always leads to sepsis shock and multiple organ dysfunction syndrome.Sepsis is the most common cause of children death,it is the hotspot and difficulty of the research field on the modem children's critical care medicine.Acute respiratory distress syndrome,is the significant cause of deterioration and death.This paper maked a review on the pathogenesis of acute respiratory distress syndrome induced by sepsis and the treatment function of Ulinastafin.

Objective To detect supernatant of SD rat bone marrow mesenchymal stem cells(BMSC)and liver cells,and explore the role of cytokines,and provide a theoretical basis for the biological artificial liver and clinical treatment of liver failure.Methods BMSCs and primary hepatocytes of SD rat were isolated.BMSCs with its third generation and liver cells by the ratio of 1∶10 were co-cultured.Hepatocytes and BMSC were treated as control group.Observation the cells′survival and morphology and analysis of cytokine after 48 h were made by RayBiotech.Results BMSC-hepatocyte in the co-culture group grow and proliferated rapidly,and hepatocyte could survive up to 14 days;while hepatocyte cultured alone,it grow slowly and survived only 9 days.Expression of BM-SC-liver cells supernatant changed obviously:interleukin 1,interleukin 6,interleukin 10 were higher than those in the control group,above 2 times,which showed significant difference (P<0.01).Conclusion Co-culture of BMSC-liver cell could induce BM-SC secrete paracrine and autocrine:IL-1 and IL-6 and IL-10,which could promote the growth of hepatocyte and extend the liver cell survival time.In-vitro BMSC-liver cells could provide biological artificial liver cell sources,as well as provide theory basis for using cytokine treatment for liver failure.

Objective To develop a fair performance appraisal method for clinical departments,as a reference for grading performance pay.Methods Ridit method with consideration of medical risks is used to evaluate clinical departments'performance.Results Both quality of care and medical risk are graded and overlaid,to arrive at the comprehensive values on which the performance pay is divided into grades Ⅰ,Ⅱ,Ⅲ,and Ⅳ.In this practice,values 7 ~8 is grade Ⅰ,6 grade Ⅱ,4 ~ 5 grade Ⅲ,and 2~3 gradeⅣ.Conclusion A fair and justifiable department performance evaluation system that is based on performance proves vital for the sustainable development of the hospital. Ridit method with consideration of medical risk evaluation advocates the performance appraisal principle of encouraging better performance,high risk and high efficiency.

Myelodysplastic syndromes (MDS) is a highly heterogeneous group of clonal disease,which is derived from hematopoietic.stem cells.Anemia is the main manifestation of most MDS patients and transfusion is required in about 90 ％ patients.Long-term blood transfusions can lead to iron overload (IOL).IOL can damage the iron balance,and excess free iron can deposite in the heart,liver and other vital organs,resulting in structural damage and organ dysfunction,which can deteriorate the prognosis,accelerate the transformation into acute myeloid leukemia,and increase the mortality.Iron chelation can improve the prognosis of patients with IOL,and prolong the overall survival and leukemia-free survival.

Objective Seek countermeasures against the debt risk in universities. Methods Taking the debts of Kunming Chenggong universities construction as an example, analyze the reasons and countermeasures for the debts of universities in Yunnan province. Results Due to the enrollment expansion policy, the higher education in Yunnan province has been transformed from the elite education into mass education. The universities in Kunming have gained unprecedented development opportunities,especially with the completion of the Kunming Chenggong University Town. However, loan funds for the new campus construction mostly came from banks. So,the huge debt stress from banks has been brought with the development of the universities. Conclusion The sustainable development of universities requires the moderate debt. The government, universities and society need to work together to control the scale of debt effectively and avoid the debt crisis.