Lentiviral vectors (LV),a special member of retroviral vectors,has become hot in gene therapy research.Studies have shown that LV can make exogenous gene expressed stably in tumor cells.As a tool for transportation,LV plays a significant role in the gene therapy of digestive system,hematologic system and gynecological tumor,which provides a promising new way for neoplasms gene therapy.

By the traditional Chinese medicine obtaining-pulse equipment coupled with pressure living like flexibility transducer and B-ultrasound scarching unit,collecting demic radial artery signal on cunkou,with the key link of four pulse tracings attributes in term of "position","rate","shape" and "force",each detecting indexes were analyzed synchronically with press pulse wave and ultrasound vas dynamic state changing trendgraph.According to the time lock analysis of electrocardiogram marking method and radial artery pulse wave,the theory approach of pulse tracings digitization were undertaken,and the scores of four attributes on pulse tracings were determined as well.

Acupuncture Points ; Acupuncture Therapy ; history ; China ; History, Ancient ; Humans ; Medicine in Literature

Automatic Data Processing ; Humans ; Medicine, Chinese Traditional ; Pulse

Objective To study the significance of anti-antibody of liver antigens in the diagnosis of autoim- mune hepatitis disease.Methods Patients were divided into three groups according the diseases:autoimmune hepatic disease 45 cases including autoimmune hepatitis(AIH)15 cases,primary biliary cirrhosis(PBC)20 cases,and prima- ry sclerotic cholangitis(PSC)10 cases;Various virus hepatitis 50 cases;Liver damage of unknown cause 30 cases.Au- to-antibody of liver antigens SLA/LP,LKM-1,LC-1,and AMA-M2 were identified by indirect immunofluorescence (IIF)assay and immunoblot assay.Results The positive rates of anti-SLA/LP(46.6%)was significantly higher than those of anti-LKM-1(13.3%),anti-LC-1(0%),and anti-AMA-M2(13.3%)in patients with AIR while these four antibodies were negative in patients with virus hepatitis.The positive rates of anti-AMA-M2 in patients of PBC and unknown liver damage were 95.0% and 6.6%,respectively.Conclusion Anti-SLA/LP is a new specific serum marker in diagnosis of AIR.The auto-antibody detection of liver antigens will be helpful to the diagnosis and therapy of autoimmune hepatic disease.

OBJECTIVE: This study was conducted to evaluate the percutaneous absorption of aconitine and mesaconitine in extracts of Radix Aconitum kusnezoffii. METHODS: The extracts of Radix Aconitum kusnezoffii were collected by using Franz diffusion cells after permeation through the skin of rats. Then rate constants of skin permeation of aconitine and mesaconitine were determined by high-performance liquid chromatography (HPLC). RESULTS: Under the condition that the concentrations of azone and propylene glycol were both 4%, the cumulative doses of skin permeation (Q) of mesaconitine and aconitine in the extracts of Radix Aconitum kusnezoffii (600 mg/ml) for 24 hours were 165.819 and 487.747 microg/cm(2) respectively, and their rate constants of skin permeation were 18.391 and 78.805 microg.cm(-2).h(-1) respectively. CONCLUSION: The aconitine and mesaconitine in the extracts of Radix Aconitum kusnezoffii can penetrate well through the skin of rats. Propylene glycol and azone can promote this penetration effects. The formula of skin permeation of mesaconitine and aconitine is in accordance with Higuchi equation and there is a linear relationship between Q and t(1/2).

Objective: To study the method of setting up,practicability and the cardiovascular physiological mechanism of slippery pulse model caused by alcohol.Methods: ①Scanned the heart and radial artery(cunkou) by the Magnetom Avanto 1.5T and record the pressure wave of radial artery by NX-6 Pulse Diagnosis Equipment after the subjects had gotten 100ml of distillate spirit of 61 degree and defined as the slippery pulse.②Record the pressure wave of carotid artery of rabbits defined as the slippery pulse after intragastric administration of distillate spirit of 40 degree 8ml per kilogram by NX-3 Pulse Diagnosis Equipment.Results: ①Slippery pulse model of human subjects: the stroke volume(SV),cardiac output(CO),cardiac index(CI),ejection fraction(EF) and the peak velocity of the blood flow increased;the end-diastole volume(EDV) and end-systole(ESV) decreased;the periph blood vessel distended and the amplitude of the offset of the axis stepped up;the characteristics of the pulse wave of cunkou were consistent with the characteristics of typical slippery pulse wave.②The characteristics of the pulse wave of slippery pulse model of rabbits were coincident with that of human subjects.Conclusion: The method of setting up slippery pulse model with alcohol is consistent with the mechanism of slippery pulse in the theory of traditional Chinese medicine and is practicable.

Accidents, Occupational ; Adult ; Arsenic Poisoning ; Critical Illness ; Fatal Outcome ; Female ; Humans

Acupuncture Points ; Acupuncture Therapy ; Adult ; Aged ; Female ; Humans ; Male ; Middle Aged ; Tennis Elbow ; therapy

Objective To study the effect of percutaneous CT-guided lung biopsy on the patients with peripheral lung focus.Methods Percutaneous CT-guided lung biopsy was performed in 38 patients with peripheral lung focus who were admitted to the Affiliated Hospital,Armed Police Medical College from Feb.2002 to Jan.2005.Results A total of 38 patients were studied,including 30 patients with lung cancer,2 patients with pulmonary tuberculosis,5 patients with benign pulmonary diseases and 1 patient with negative result.The positive rate was 97.36% in the 38 cases with a slight side effect.Conclusion This method is simple,safe and effective,and it is very important in diagnosis of patients with peripheral lung focus.