Objective To study the regulatory effect of TGF ? 1 on the expression of multiple genes in prostatic stromal cells in vitro. Methods The primary culture of prostatic stromal cells (including fibroblasts and smooth muscle cells ) have been established and cultured to 4～6 passages. Then the cells were cultured in the medium with various concentration of TGF ? 1(0.01, 0.10, 1.00 and 10.0 ng/ml)for 48h. By semi quantitative RT PCR method, the androgen receptor(AR), TGF ? 1, bFGF and smoothlin mRNA were measured. Results The expression of AR could be stimulated by low concentration of TGF ? 1 ( P

Acupuncture Points ; Acupuncture Therapy ; Adolescent ; Adult ; Aged ; Chest Pain ; therapy ; Exercise Therapy ; Female ; Humans ; Male ; Middle Aged ; Young Adult

In recent years, with the development of neuroimaging and the advancement in related studies, people have had a deeper understanding of perimesencephalic nonaneurysmal subarachnoid hemorrhage. This article reviews the progress in research on the etiology, onset characteristics, imaging features, diagnosis and prognosis of perimesencephalic nonaneurysmal subarachnoid hemorrhage.

Antineoplastic Agents ; therapeutic use ; BRCA2 Protein ; metabolism ; Breast Neoplasms ; drug therapy ; metabolism ; pathology ; Cell Cycle Proteins ; metabolism ; physiology ; Centrosome ; metabolism ; Drug Screening Assays, Antitumor ; Female ; Humans ; Microtubule-Associated Proteins ; metabolism ; Neoplasm Invasiveness ; Nuclear Proteins ; metabolism ; Phosphorylation ; Prognosis ; Protein-Serine-Threonine Kinases ; metabolism ; physiology ; Proto-Oncogene Proteins ; metabolism ; physiology ; RNA, Small Interfering ; pharmacology ; Tumor Suppressor Protein p53 ; metabolism

Aneuploidy ; Breast Neoplasms ; genetics ; metabolism ; pathology ; Carcinoma in Situ ; genetics ; metabolism ; pathology ; Carcinoma, Ductal, Breast ; genetics ; metabolism ; pathology ; Cell Movement ; DNA Methylation ; DNA, Neoplasm ; genetics ; Female ; Humans ; Membrane Proteins ; metabolism ; Neoplasm Invasiveness

Objective:To study the determination method of scutellarin in BREVISCAPINE INJECTION using HPLC.Methods:The waters ODS C 18 column (5?m,3.9?150mm) was used. The methanol water glacial acetic acid (40∶60∶1) was used as a mobile phase. The detection wavelength of scutellarin was set at 335nm and internal standard baicalin at 280nm. The resolution of scutellarin's and baicalin peaks was at least 5. Number of theoretical plates of column is no less than 1000 to scutellarin. Results:The recovery of additive sample is 99.8%. RSD is no more 2.0%.Conclusion: It is generally in agreement with external standard method.

Objective To explore the current status and influencing factors of oral health knowledge,attitudes and practice (KAP) among the teachers of primary and secondary schools in Gongshu District of Hangzhou City.Methods A total of 652 teachers of primary and secondary schools were selected by using multi-stage stratified cluster random sampling method and completed a self-designed questionnaire,which included demographic characteristics and oral health KAP.The status and influencing factors of oral health KAP were analyzed by single and multiple-factor analysis methods.Results The questionnaire was completed by all the 652 teachers,627 of whom were qualified (96.2％).The total ratios of oral health knowledge awareness,attitudes correctness and behaviors formation were 77.1％,68.7％ and 46.3％,respectively.There was significant difference of knowledge awareness rate by age,gender,marriage status,monthly income,life status and current cigarette smoking (x2 values were 11.48,8.57,8.12,8.46,23.97 and 4.64,respectively; all P＜0.05).There was significant difference in oral health attitudes correctness rate by gender (x2=10.65,P＜0.05) and behaviors formation rate by gender,education level and life status (x2 values were 3.95,10.88 and 10.41,respectively; all P＜0.05).In logistic regression model,impact factors of oral health knowledge awareness were age,gender,life status and physical exercises; the impact factors of oral health attitudes correctness were gender and monthly income; the impact factors of oral health behaviors formation were gender,level of education and physical exercises.Conclusion The oral health awareness and attitudes correctness are acceptible among the teachers of primary and secondary schools in Gongshu District of Hangzhou City,although the formation rate of oral health behaviors is low.Oral health education should be enforced among the teachers of primary and secondary schools.

OBJECTIVETo observe the effect of Chinese drugs of Jianpi Huayu (JPHY, strengthening Pi and dissolving stasis) on healing quality of gastric ulcer and its mechanism.

METHODSThe gastric ulcer model was established by subserous injection of ethanoic acid in rats. Rats were randomly divided into 4 groups, the blank group, the model group, the ranitidine (RT) group and the JPHY group. Quantity of regenerative mucosa of healed gastric ulcer was determined using HE stain, epidermal growth factor (EGF) content in serum and stomach mucosa was detected by RIA and epidermal growth factor receptor (EGFR) protein expression was determined by immunohistochemistry.

RESULTSThickness of regenerated mucosa in the CHM group was higher than that in the model group and the RT group (P<0.05 or P<0.01); EGF content in mucosa in the JPHY group and the RT group was higher than that in the model group (P<0.01) and EGFR protein expression in the JPHY group was higher than that in the model group (P<0.05).

CONCLUSIONJPHY could improve the proliferation of epithelial cells, inhibit gastric acid, improve microcirculation of gastric mucosa through the mediation of EGFR, so as to elevate the healing quality of gastric ulcer, display its anti-ulcer action.

Acetic Acid ; Animals ; Anti-Ulcer Agents ; pharmacology ; Drugs, Chinese Herbal ; pharmacology ; Epidermal Growth Factor ; metabolism ; Gastric Mucosa ; metabolism ; pathology ; physiopathology ; Male ; Random Allocation ; Rats ; Rats, Wistar ; Receptor, Epidermal Growth Factor ; metabolism ; Stomach Ulcer ; chemically induced ; physiopathology

OBJECTIVETo observe the efficacy differences among acupuncture at Neiguan (PC 6) and Zusanli (ST 36), dolantin and scopolamine in treatment of acute renal colic, and to verify the clinical effect of acupuncture at Neiguan (PC 6) and Zusanli (ST 36).

METHODSTwo hundred and forty patients were randomly divided into an acupuncture group, a scopolamine group and a dolantin group, 80 cases in each group. The acupuncture group was treated by acupuncture at unilateral or bilateral Neiguan (PC 6) and Zusanli (ST 36). The scopolamine group was treated by intramuscular injection of scopolamine combined with intravenous drip of scopolamine, and the dolantin group was treated by intramuscular injection of dolantin. The clinical therapeutic effects were observed 30 min after drug administration or acupuncture, and the onset time of effect and adverse reactions were compared among the groups.

RESULTSThe total effective rate of 95.0% (76/80) in the acupuncture group had a similar therapeutic effect to 92.5% (74/80) in dolantin group (P>0.05), which were both superior to 76.3% (61/80) in scopolamine group (both P<0.05). The onset time of (13.24 +/- 2.12) min in the acupuncture group was significant earlier than (23.11 +/- 6.22) min in scopolamine group and (22.17 +/- 3.15) min in dolantin group (both P<0.05), and compared with the other two groups, the adverse reactions in the acupuncture group were significant decreased (both P<0.05).

CONCLUSIONAcupuncture at Neiguan (PC 6) and Zusanli (ST 36) has a good analgesic effect with a low incidence of adverse reactions, which is superior to that of dolantin and scopolamine.

Acupuncture Points ; Acupuncture Therapy ; Adolescent ; Adult ; Aged ; Female ; Humans ; Male ; Middle Aged ; Renal Colic ; therapy ; Treatment Outcome ; Young Adult

Objective To investigate the epiderniology of fungemia and provide evidence for clinical therapy.Methods A retro- spective survey was done with the 31 cases of fungemia in our hospital from August 2004 to November 2005.Results More than 80% of the patients suffered from two and more underlying diseases.Over a half of infections developed following placement of catheters.And 83.9% of the patients had a history of antimicrobial agents use before blood culture.The pathogens of 24 (77.4%) cases were associated with Candida spp.Only 3 strains were C.albicans.The mortality rate of candidemia was 45.8%.Different Candida species had different resistance rates to antifungal agents.Conclusions Fungemia patients often have serious underlying diseases.Most fungemia cases were candidemia caused by non-C.albicans.Some fungal pathogens are re- sistant to fluconazole and itraconazole.